The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats
Background: Fatty diet increases apoptosis and cardiovascular disease. Therefore, this study aimed to evaluate the effect of 4-week aerobic exercise along with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats. Materials and Methods: This is an exp...
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Kashan University of Medical Sciences and Health Services
2022-01-01
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| Series: | Fiyz̤ |
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| Online Access: | http://feyz.kaums.ac.ir/article-1-4631-en.html |
| _version_ | 1811175265623080960 |
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| author | Mohammad Kazemipour Hasan Mateen Homaie Parvin Farzanegi |
| author_facet | Mohammad Kazemipour Hasan Mateen Homaie Parvin Farzanegi |
| author_sort | Mohammad Kazemipour |
| collection | DOAJ |
| description | Background: Fatty diet increases apoptosis and cardiovascular disease. Therefore, this study aimed to evaluate the effect of 4-week aerobic exercise along with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats.
Materials and Methods: This is an experimental study. 12-week-old female rats were randomly divided into 5 groups of 6; Positive control (healthy), negative control (obesity), exercise + obesity, extract + obesity, exercise and extract + obesity (interaction of exercise and extract). Training was done for 4 weeks with moderate intensity of 50-60% Vo2max. Pistachio skin extract was used at a dose of 60 mg per kilogram. qPCR method was used to check gene expression.
Results: The results showed that the expression of bax and caspase 3 was higher in the obese group than in the healthy group (P=0.001). The extract groups, the exercise group and the exercise + extract group had more bax and caspase 3 expression compared to the obese group (P=0.001). The expression of Bcl-2 in the obese group and the exercise group was lower than the healthy group (P=0.001). Also, the expression of Bcl-2 was higher in the exercise + extract group than in the obese group (P=0.001). There was a significant difference between the exercise group and the exercise+extract group with the extract group.
Conclusion: High-fat diet increase bax and caspase 3 and decreased Bcl-2 in rats. However, exercise and consumption of green pistachio skin extract together decrease bax and caspase 3 and increased Bcl-2. |
| first_indexed | 2024-04-10T19:33:00Z |
| format | Article |
| id | doaj.art-6450fb92bf13471693693a49af6d4ad6 |
| institution | Directory Open Access Journal |
| issn | 1029-7855 2008-9821 |
| language | fas |
| last_indexed | 2024-04-10T19:33:00Z |
| publishDate | 2022-01-01 |
| publisher | Kashan University of Medical Sciences and Health Services |
| record_format | Article |
| series | Fiyz̤ |
| spelling | doaj.art-6450fb92bf13471693693a49af6d4ad62023-01-30T09:38:26ZfasKashan University of Medical Sciences and Health ServicesFiyz̤1029-78552008-98212022-01-01265521529The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed ratsMohammad Kazemipour Hasan Mateen Homaie 0Parvin FarzanegiDepartment of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, I.R. Iran.Background: Fatty diet increases apoptosis and cardiovascular disease. Therefore, this study aimed to evaluate the effect of 4-week aerobic exercise along with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats. Materials and Methods: This is an experimental study. 12-week-old female rats were randomly divided into 5 groups of 6; Positive control (healthy), negative control (obesity), exercise + obesity, extract + obesity, exercise and extract + obesity (interaction of exercise and extract). Training was done for 4 weeks with moderate intensity of 50-60% Vo2max. Pistachio skin extract was used at a dose of 60 mg per kilogram. qPCR method was used to check gene expression. Results: The results showed that the expression of bax and caspase 3 was higher in the obese group than in the healthy group (P=0.001). The extract groups, the exercise group and the exercise + extract group had more bax and caspase 3 expression compared to the obese group (P=0.001). The expression of Bcl-2 in the obese group and the exercise group was lower than the healthy group (P=0.001). Also, the expression of Bcl-2 was higher in the exercise + extract group than in the obese group (P=0.001). There was a significant difference between the exercise group and the exercise+extract group with the extract group. Conclusion: High-fat diet increase bax and caspase 3 and decreased Bcl-2 in rats. However, exercise and consumption of green pistachio skin extract together decrease bax and caspase 3 and increased Bcl-2.http://feyz.kaums.ac.ir/article-1-4631-en.htmlhigh fat dietaerobic exercisepistachio skinapoptosis |
| spellingShingle | Mohammad Kazemipour Hasan Mateen Homaie Parvin Farzanegi The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats Fiyz̤ high fat diet aerobic exercise pistachio skin apoptosis |
| title | The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats |
| title_full | The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats |
| title_fullStr | The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats |
| title_full_unstemmed | The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats |
| title_short | The effect of aerobic exercise with pistachio skin extract on the expression of Bax, caspase-3 and Bcl-2 in heart tissue of fat-fed rats |
| title_sort | effect of aerobic exercise with pistachio skin extract on the expression of bax caspase 3 and bcl 2 in heart tissue of fat fed rats |
| topic | high fat diet aerobic exercise pistachio skin apoptosis |
| url | http://feyz.kaums.ac.ir/article-1-4631-en.html |
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