Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)
Abstract Background Water chickweed (Myosoton aquaticum (L.)) is a dicot broadleaf weed that is widespread in winter fields in China, and has evolved serious resistance to acetolactate synthase (ALS) inhibiting herbicides. Results We identified a M. aquaticum population exhibiting moderate (6.15-fol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1451-x |
| _version_ | 1811289286917488640 |
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| author | Weitang Liu Shuang Bai Ning Zhao Sisi Jia Wei Li Lele Zhang Jinxin Wang |
| author_facet | Weitang Liu Shuang Bai Ning Zhao Sisi Jia Wei Li Lele Zhang Jinxin Wang |
| author_sort | Weitang Liu |
| collection | DOAJ |
| description | Abstract Background Water chickweed (Myosoton aquaticum (L.)) is a dicot broadleaf weed that is widespread in winter fields in China, and has evolved serious resistance to acetolactate synthase (ALS) inhibiting herbicides. Results We identified a M. aquaticum population exhibiting moderate (6.15-fold) resistance to tribenuron-methyl (TM). Target-site ALS gene sequencing revealed no known resistance mutations in these plants, and the in vitro ALS activity assays showed no differences in enzyme sensitivity between susceptible and resistant populations; however, resistance was reversed by pretreatment with the cytochrome P450 (CYP) monooxygenase inhibitor malathion. An RNA sequencing transcriptome analysis was performed to identify candidate genes involved in metabolic resistance, and the unigenes obtained by de novo transcriptome assembly were annotated across seven databases. In total, 34 differentially expressed genes selected by digital gene expression analysis were validated by quantitative real-time (qRT)-PCR. Ten consistently overexpressed contigs, including four for CYP, four for ATP-binding cassette (ABC) transporter, and two for peroxidase were further validated by qRT-PCR using additional plants from resistant and susceptible populations. Three CYP genes (with homology to CYP734A1, CYP76C1, and CYP86B1) and one ABC transporter gene (with homology to ABCC10) were highly expressed in all resistant plants. Conclusion The mechanism of TM resistance in M. aquaticum is controlled by NTSR rather than TSR. Four genes, CYP734A1, CYP76C1, CYP86B1, and ABCC10 could play essential role in metabolic resistance to TM and justify further functional studies. To our knowledge, this is the first large-scale transcriptome analysis of genes associated with NTSR in M. aquaticum using the Illumina platform. Our data provide resource for M. aquaticum biology, and will facilitate the study of herbicide resistance mechanism at the molecular level in this species as well as in other weeds. |
| first_indexed | 2024-04-13T03:52:16Z |
| format | Article |
| id | doaj.art-6451778ec925471ba06db5229e82fd7b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T03:52:16Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-6451778ec925471ba06db5229e82fd7b2022-12-22T03:03:45ZengBMCBMC Plant Biology1471-22292018-10-0118111410.1186/s12870-018-1451-xNon-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)Weitang Liu0Shuang Bai1Ning Zhao2Sisi Jia3Wei Li4Lele Zhang5Jinxin Wang6Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityKey Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityKey Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityTaian CustomsKey Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityKey Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityKey Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural UniversityAbstract Background Water chickweed (Myosoton aquaticum (L.)) is a dicot broadleaf weed that is widespread in winter fields in China, and has evolved serious resistance to acetolactate synthase (ALS) inhibiting herbicides. Results We identified a M. aquaticum population exhibiting moderate (6.15-fold) resistance to tribenuron-methyl (TM). Target-site ALS gene sequencing revealed no known resistance mutations in these plants, and the in vitro ALS activity assays showed no differences in enzyme sensitivity between susceptible and resistant populations; however, resistance was reversed by pretreatment with the cytochrome P450 (CYP) monooxygenase inhibitor malathion. An RNA sequencing transcriptome analysis was performed to identify candidate genes involved in metabolic resistance, and the unigenes obtained by de novo transcriptome assembly were annotated across seven databases. In total, 34 differentially expressed genes selected by digital gene expression analysis were validated by quantitative real-time (qRT)-PCR. Ten consistently overexpressed contigs, including four for CYP, four for ATP-binding cassette (ABC) transporter, and two for peroxidase were further validated by qRT-PCR using additional plants from resistant and susceptible populations. Three CYP genes (with homology to CYP734A1, CYP76C1, and CYP86B1) and one ABC transporter gene (with homology to ABCC10) were highly expressed in all resistant plants. Conclusion The mechanism of TM resistance in M. aquaticum is controlled by NTSR rather than TSR. Four genes, CYP734A1, CYP76C1, CYP86B1, and ABCC10 could play essential role in metabolic resistance to TM and justify further functional studies. To our knowledge, this is the first large-scale transcriptome analysis of genes associated with NTSR in M. aquaticum using the Illumina platform. Our data provide resource for M. aquaticum biology, and will facilitate the study of herbicide resistance mechanism at the molecular level in this species as well as in other weeds.http://link.springer.com/article/10.1186/s12870-018-1451-xAcetolactate synthaseNon-target site-based resistanceMetabolic resistanceRNA-SeqTribenuron-methylMyosoton aquaticum (L.) |
| spellingShingle | Weitang Liu Shuang Bai Ning Zhao Sisi Jia Wei Li Lele Zhang Jinxin Wang Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) BMC Plant Biology Acetolactate synthase Non-target site-based resistance Metabolic resistance RNA-Seq Tribenuron-methyl Myosoton aquaticum (L.) |
| title | Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) |
| title_full | Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) |
| title_fullStr | Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) |
| title_full_unstemmed | Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) |
| title_short | Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.) |
| title_sort | non target site based resistance to tribenuron methyl and essential involved genes in myosoton aquaticum l |
| topic | Acetolactate synthase Non-target site-based resistance Metabolic resistance RNA-Seq Tribenuron-methyl Myosoton aquaticum (L.) |
| url | http://link.springer.com/article/10.1186/s12870-018-1451-x |
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