Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production
<i>Bacillus subtilis</i> (Bs) is a bacterium that benefits plants and is used in the production of bio-fungicides. The cultivation of Bs is a crucial step in bio-control preparation production, as it greatly impacts the quality and price of the final product. In a series of shake flask e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/4/390 |
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| author | Oskars Grigs Elina Didrihsone Emils Bolmanis |
| author_facet | Oskars Grigs Elina Didrihsone Emils Bolmanis |
| author_sort | Oskars Grigs |
| collection | DOAJ |
| description | <i>Bacillus subtilis</i> (Bs) is a bacterium that benefits plants and is used in the production of bio-fungicides. The cultivation of Bs is a crucial step in bio-control preparation production, as it greatly impacts the quality and price of the final product. In a series of shake flask experiments, we investigated the economically feasible broth composition for spore production of <i>Bacillus subtilis</i> MSCL 897, a Latvian soil isolate. Our study investigated the impact of utilizing legume-based flours (such as broad bean, grey pea, and soybean) as the primary nitrogen source, along with sugar-beet molasses, sucrose, or glucose as the carbon source, and yeast extract, peptone, and corn-steep liquor as growth factor additives. Additionally, we examined the effect of using (NH<sub>4</sub>)<sub>2</sub>HPO<sub>4</sub> or urea as supplementary nitrogen sources, as well as previously established media formulations, on spore yield. Our results showed that a culture medium composed of broad bean flour (10 g/L) and molasses (10 g/L) led to spore productivity of 1.35 ± 0.47 × 10<sup>8</sup> CFU/mL at 48 h. By enriching the culture medium base constituents with a minor (0.5–1.0 g/L) yeast extract or corn-steep liquor additive, a notable increase in spore productivity was observed, with values of 2.00 ± 0.28 × 10<sup>8</sup> and 2.34 ± 0.18 × 10<sup>8</sup> CFU/mL at 48 h, respectively, and sporulation efficiency > 80–90%. As a result, we achieved a high spore yield of the <i>Bacillus subtilis</i> MSCL 897 strain, demonstrating the competitiveness of our approach, which relied on a low-cost medium made mainly from locally available and renewable raw materials. |
| first_indexed | 2024-03-11T05:02:04Z |
| format | Article |
| id | doaj.art-6451ba528fa14dbe877688b435a940a3 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T05:02:04Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-6451ba528fa14dbe877688b435a940a32023-11-17T19:11:14ZengMDPI AGFermentation2311-56372023-04-019439010.3390/fermentation9040390Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore ProductionOskars Grigs0Elina Didrihsone1Emils Bolmanis2Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaLatvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaLatvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, Latvia<i>Bacillus subtilis</i> (Bs) is a bacterium that benefits plants and is used in the production of bio-fungicides. The cultivation of Bs is a crucial step in bio-control preparation production, as it greatly impacts the quality and price of the final product. In a series of shake flask experiments, we investigated the economically feasible broth composition for spore production of <i>Bacillus subtilis</i> MSCL 897, a Latvian soil isolate. Our study investigated the impact of utilizing legume-based flours (such as broad bean, grey pea, and soybean) as the primary nitrogen source, along with sugar-beet molasses, sucrose, or glucose as the carbon source, and yeast extract, peptone, and corn-steep liquor as growth factor additives. Additionally, we examined the effect of using (NH<sub>4</sub>)<sub>2</sub>HPO<sub>4</sub> or urea as supplementary nitrogen sources, as well as previously established media formulations, on spore yield. Our results showed that a culture medium composed of broad bean flour (10 g/L) and molasses (10 g/L) led to spore productivity of 1.35 ± 0.47 × 10<sup>8</sup> CFU/mL at 48 h. By enriching the culture medium base constituents with a minor (0.5–1.0 g/L) yeast extract or corn-steep liquor additive, a notable increase in spore productivity was observed, with values of 2.00 ± 0.28 × 10<sup>8</sup> and 2.34 ± 0.18 × 10<sup>8</sup> CFU/mL at 48 h, respectively, and sporulation efficiency > 80–90%. As a result, we achieved a high spore yield of the <i>Bacillus subtilis</i> MSCL 897 strain, demonstrating the competitiveness of our approach, which relied on a low-cost medium made mainly from locally available and renewable raw materials.https://www.mdpi.com/2311-5637/9/4/390<i>Bacillus subtilis</i>low-cost medium/brothendosporesbio-fungicide |
| spellingShingle | Oskars Grigs Elina Didrihsone Emils Bolmanis Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production Fermentation <i>Bacillus subtilis</i> low-cost medium/broth endospores bio-fungicide |
| title | Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production |
| title_full | Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production |
| title_fullStr | Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production |
| title_full_unstemmed | Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production |
| title_short | Investigation of a Broad-Bean Based Low-Cost Medium Formulation for <i>Bacillus subtilis</i> MSCL 897 Spore Production |
| title_sort | investigation of a broad bean based low cost medium formulation for i bacillus subtilis i mscl 897 spore production |
| topic | <i>Bacillus subtilis</i> low-cost medium/broth endospores bio-fungicide |
| url | https://www.mdpi.com/2311-5637/9/4/390 |
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