A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis
Hemoglobin is a biomarker of interest for the diagnosis and prognosis of various diseases such as anemia, sickle cell disease, and thalassemia. In this paper, we present a disposable device that has the potential of being used in a setting for accurately quantifying hemoglobin levels in whole blood...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/1/394 |
| _version_ | 1797431146465722368 |
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| author | Zhuolun Meng Muhammad Tayyab Zhongtian Lin Hassan Raji Mehdi Javanmard |
| author_facet | Zhuolun Meng Muhammad Tayyab Zhongtian Lin Hassan Raji Mehdi Javanmard |
| author_sort | Zhuolun Meng |
| collection | DOAJ |
| description | Hemoglobin is a biomarker of interest for the diagnosis and prognosis of various diseases such as anemia, sickle cell disease, and thalassemia. In this paper, we present a disposable device that has the potential of being used in a setting for accurately quantifying hemoglobin levels in whole blood based on colorimetric analysis using a smartphone camera. Our biosensor employs a disposable microfluidic chip which is made using medical-grade tapes and filter paper on a glass slide in conjunction with a custom-made PolyDimethylSiloaxane (PDMS) micropump for enhancing capillary flow. Once the blood flows through the device, the glass slide is imaged using a smartphone equipped with a custom 3D printed attachment. The attachment has a Light Emitting Diode (LED) that functions as an independent light source to reduce the noise caused by background illumination and external light sources. We then use the RGB values obtained from the image to quantify the hemoglobin levels. We demonstrated the capability of our device for quantifying hemoglobin in Bovine Hemoglobin Powder, Frozen Beef Blood, and human blood. We present a logarithmic model that specifies the relationship between the Red channel of the RGB values and Hemoglobin concentration. |
| first_indexed | 2024-03-09T09:40:42Z |
| format | Article |
| id | doaj.art-64527da19aa04d05a8fc124b6cb286ae |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T09:40:42Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-64527da19aa04d05a8fc124b6cb286ae2023-12-02T00:56:27ZengMDPI AGSensors1424-82202022-12-0123139410.3390/s23010394A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric AnalysisZhuolun Meng0Muhammad Tayyab1Zhongtian Lin2Hassan Raji3Mehdi Javanmard4Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USADepartment of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USADepartment of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USADepartment of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USADepartment of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854, USAHemoglobin is a biomarker of interest for the diagnosis and prognosis of various diseases such as anemia, sickle cell disease, and thalassemia. In this paper, we present a disposable device that has the potential of being used in a setting for accurately quantifying hemoglobin levels in whole blood based on colorimetric analysis using a smartphone camera. Our biosensor employs a disposable microfluidic chip which is made using medical-grade tapes and filter paper on a glass slide in conjunction with a custom-made PolyDimethylSiloaxane (PDMS) micropump for enhancing capillary flow. Once the blood flows through the device, the glass slide is imaged using a smartphone equipped with a custom 3D printed attachment. The attachment has a Light Emitting Diode (LED) that functions as an independent light source to reduce the noise caused by background illumination and external light sources. We then use the RGB values obtained from the image to quantify the hemoglobin levels. We demonstrated the capability of our device for quantifying hemoglobin in Bovine Hemoglobin Powder, Frozen Beef Blood, and human blood. We present a logarithmic model that specifies the relationship between the Red channel of the RGB values and Hemoglobin concentration.https://www.mdpi.com/1424-8220/23/1/394biosensorhemoglobinlab-on-a-chipmedical tapemicropumprapid test |
| spellingShingle | Zhuolun Meng Muhammad Tayyab Zhongtian Lin Hassan Raji Mehdi Javanmard A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis Sensors biosensor hemoglobin lab-on-a-chip medical tape micropump rapid test |
| title | A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis |
| title_full | A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis |
| title_fullStr | A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis |
| title_full_unstemmed | A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis |
| title_short | A Smartphone-Based Disposable Hemoglobin Sensor Based on Colorimetric Analysis |
| title_sort | smartphone based disposable hemoglobin sensor based on colorimetric analysis |
| topic | biosensor hemoglobin lab-on-a-chip medical tape micropump rapid test |
| url | https://www.mdpi.com/1424-8220/23/1/394 |
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