Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials
The antibacterial activity, and the effect of the application of additional topical fluoride on the bacterial activity, biofilm formation, and surface roughness of new-generation hybrid/fluoride-releasing materials were investigated. Two hundred and forty specimens were prepared in split Teflon mold...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/5/2434 |
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| author | Sevil Gurgan Uzay Koc Vural Cansu Atalay Herve Tassery Ivana Miletic Suna Sibel Gurpinar |
| author_facet | Sevil Gurgan Uzay Koc Vural Cansu Atalay Herve Tassery Ivana Miletic Suna Sibel Gurpinar |
| author_sort | Sevil Gurgan |
| collection | DOAJ |
| description | The antibacterial activity, and the effect of the application of additional topical fluoride on the bacterial activity, biofilm formation, and surface roughness of new-generation hybrid/fluoride-releasing materials were investigated. Two hundred and forty specimens were prepared in split Teflon molds (8 × 2 mm) from a resin composite (as negative control: G-aenial A’Chord/GC), Equia Forte HT Fil(GC), Equia Forte HT Fil+Equia Forte Coat, Riva Self-Cure (SDI), Riva Self-Cure+Equia Forte Coat, Zirconomer (Shofu), Beautifil II (Shofu), and Riva Silver (Shofu). Penicillin G,1U was used as positive control. The antibacterial activity was evaluated by the agar diffusion test immediately after the materials set using <i>Streptococcus mutans</i> (<i>S. mutans</i>) and <i>Lactobacillus casei</i> (<i>L. casei)</i>, and repeated after application of 0.20% <i>w/w</i> (900 ppm) topical fluoride. The biofilm formation of <i>S. mutans</i> on each material was quantified by crystal violet staining. Surface roughness of the specimens was measured by a profilometer. The data were analyzed by Kruskal–Wallis, Dunn’s, one-way ANOVA, and Tukey’s HSD tests (<i>p</i> < 0.05). None of the tested restorative materials showed antibacterial activity and no inhibition zones were observed after treatment of the restoratives with additional topical fluoride. There were significant differences among the groups in terms of biofilm formation (<i>p</i> < 0.005). Equia Forte HT Fil with and without coating showed the lowest, while Riva self-cure without coating and Zirconomer showed the highest biofilm accumulation. None of the new-generation hybrid/fluoride-releasing materials demonstrated antibacterial activity and additional topical fluoride application did not make any change. Biofilm formation of the tested materials differed. All tested materials showed different surface roughness values (<i>p</i> < 0.005). Characteristics and compositions of the materials seemed to be more effective than the surface roughness. |
| first_indexed | 2024-03-09T20:48:36Z |
| format | Article |
| id | doaj.art-64539f169b9548bc9bc0c8651f857add |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:48:36Z |
| publishDate | 2022-02-01 |
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| series | Applied Sciences |
| spelling | doaj.art-64539f169b9548bc9bc0c8651f857add2023-11-23T22:40:43ZengMDPI AGApplied Sciences2076-34172022-02-01125243410.3390/app12052434Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative MaterialsSevil Gurgan0Uzay Koc Vural1Cansu Atalay2Herve Tassery3Ivana Miletic4Suna Sibel Gurpinar5Department of Restorative Dentistry, School of Dentistry, Hacettepe University, 06100 Ankara, TurkeyDepartment of Restorative Dentistry, School of Dentistry, Hacettepe University, 06100 Ankara, TurkeyDepartment of Restorative Dentistry, School of Dentistry, Hacettepe University, 06100 Ankara, TurkeyEcole de Médecine Dentaire de Marseille, Université d’Aix-Marseille, 13385 Marseille, FranceDepartments of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, 10000 Zagreb, CroatiaDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Ankara University, 06560 Ankara, TurkeyThe antibacterial activity, and the effect of the application of additional topical fluoride on the bacterial activity, biofilm formation, and surface roughness of new-generation hybrid/fluoride-releasing materials were investigated. Two hundred and forty specimens were prepared in split Teflon molds (8 × 2 mm) from a resin composite (as negative control: G-aenial A’Chord/GC), Equia Forte HT Fil(GC), Equia Forte HT Fil+Equia Forte Coat, Riva Self-Cure (SDI), Riva Self-Cure+Equia Forte Coat, Zirconomer (Shofu), Beautifil II (Shofu), and Riva Silver (Shofu). Penicillin G,1U was used as positive control. The antibacterial activity was evaluated by the agar diffusion test immediately after the materials set using <i>Streptococcus mutans</i> (<i>S. mutans</i>) and <i>Lactobacillus casei</i> (<i>L. casei)</i>, and repeated after application of 0.20% <i>w/w</i> (900 ppm) topical fluoride. The biofilm formation of <i>S. mutans</i> on each material was quantified by crystal violet staining. Surface roughness of the specimens was measured by a profilometer. The data were analyzed by Kruskal–Wallis, Dunn’s, one-way ANOVA, and Tukey’s HSD tests (<i>p</i> < 0.05). None of the tested restorative materials showed antibacterial activity and no inhibition zones were observed after treatment of the restoratives with additional topical fluoride. There were significant differences among the groups in terms of biofilm formation (<i>p</i> < 0.005). Equia Forte HT Fil with and without coating showed the lowest, while Riva self-cure without coating and Zirconomer showed the highest biofilm accumulation. None of the new-generation hybrid/fluoride-releasing materials demonstrated antibacterial activity and additional topical fluoride application did not make any change. Biofilm formation of the tested materials differed. All tested materials showed different surface roughness values (<i>p</i> < 0.005). Characteristics and compositions of the materials seemed to be more effective than the surface roughness.https://www.mdpi.com/2076-3417/12/5/2434fluoride-releasing materialsantibacterial activitybiofilm formationsurface roughness<i>S. mutans</i><i>L. casei</i> |
| spellingShingle | Sevil Gurgan Uzay Koc Vural Cansu Atalay Herve Tassery Ivana Miletic Suna Sibel Gurpinar Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials Applied Sciences fluoride-releasing materials antibacterial activity biofilm formation surface roughness <i>S. mutans</i> <i>L. casei</i> |
| title | Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials |
| title_full | Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials |
| title_fullStr | Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials |
| title_full_unstemmed | Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials |
| title_short | Antibacterial Activity and Biofilm Inhibition of New-Generation Hybrid/Fluoride-Releasing Restorative Materials |
| title_sort | antibacterial activity and biofilm inhibition of new generation hybrid fluoride releasing restorative materials |
| topic | fluoride-releasing materials antibacterial activity biofilm formation surface roughness <i>S. mutans</i> <i>L. casei</i> |
| url | https://www.mdpi.com/2076-3417/12/5/2434 |
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