Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant
In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CD...
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/1/133 |
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| author | A-Ling Chen Xiao-Yan Wang Qing Zhang Ning Bao Shou-Nian Ding |
| author_facet | A-Ling Chen Xiao-Yan Wang Qing Zhang Ning Bao Shou-Nian Ding |
| author_sort | A-Ling Chen |
| collection | DOAJ |
| description | In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO<sub>2</sub> NPs) to form a co-reactant of Ru(bpy)<sub>3</sub><sup>2+</sup>. Subsequently, a sandwich immunosensor for detecting human chorionic gonadotropin (HCG) was constructed based on CDs@dSiO<sub>2</sub> NPs as co-reactants, the nanoprobe loaded with the secondary antibody, and Ru(bpy)<sub>3</sub><sup>2+</sup> as a luminophore. In addition, compared to directly as a signal probe, the luminophore Ru (bpy)<sub>3</sub><sup>2+</sup> as a part of the electrolyte solution is simpler in this work. The immunosensor has an extremely low limit of detection of 0.00019 mIU/mL. This work describes the synthesis of low-toxic, efficient, and environmentally friendly CDs, which have become ideal co-reactants of Ru(bpy)<sub>3</sub><sup>2+</sup>, and proposes an ECL immunosensor with excellent stability and selectivity, which has great potential in clinical applications. |
| first_indexed | 2024-03-09T13:24:20Z |
| format | Article |
| id | doaj.art-645505a436604d9bbe041a85f4074275 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:24:20Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-645505a436604d9bbe041a85f40742752023-11-30T21:25:59ZengMDPI AGBiosensors2079-63742023-01-0113113310.3390/bios13010133Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-ReactantA-Ling Chen0Xiao-Yan Wang1Qing Zhang2Ning Bao3Shou-Nian Ding4Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, ChinaJiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, ChinaKey Laboratory of Consumer Product Quality Safety Inspection and Risk Assessment for State Market Regulation, Chinese Academy of Inspection and Quarantine, Beijing 100176, ChinaSchool of Public Health, Nantong University, Nantong 226019, ChinaJiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, ChinaIn general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO<sub>2</sub> NPs) to form a co-reactant of Ru(bpy)<sub>3</sub><sup>2+</sup>. Subsequently, a sandwich immunosensor for detecting human chorionic gonadotropin (HCG) was constructed based on CDs@dSiO<sub>2</sub> NPs as co-reactants, the nanoprobe loaded with the secondary antibody, and Ru(bpy)<sub>3</sub><sup>2+</sup> as a luminophore. In addition, compared to directly as a signal probe, the luminophore Ru (bpy)<sub>3</sub><sup>2+</sup> as a part of the electrolyte solution is simpler in this work. The immunosensor has an extremely low limit of detection of 0.00019 mIU/mL. This work describes the synthesis of low-toxic, efficient, and environmentally friendly CDs, which have become ideal co-reactants of Ru(bpy)<sub>3</sub><sup>2+</sup>, and proposes an ECL immunosensor with excellent stability and selectivity, which has great potential in clinical applications.https://www.mdpi.com/2079-6374/13/1/133carbon dotsRu(bpy)<sub>3</sub><sup>2+</sup>electrochemiluminescenceimmunosensor |
| spellingShingle | A-Ling Chen Xiao-Yan Wang Qing Zhang Ning Bao Shou-Nian Ding Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant Biosensors carbon dots Ru(bpy)<sub>3</sub><sup>2+</sup> electrochemiluminescence immunosensor |
| title | Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant |
| title_full | Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant |
| title_fullStr | Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant |
| title_full_unstemmed | Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant |
| title_short | Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO<sub>2</sub> Nanoparticles as Nanoprobe and Co-Reactant |
| title_sort | sandwich type electrochemiluminescence immunosensor based on cds dsio sub 2 sub nanoparticles as nanoprobe and co reactant |
| topic | carbon dots Ru(bpy)<sub>3</sub><sup>2+</sup> electrochemiluminescence immunosensor |
| url | https://www.mdpi.com/2079-6374/13/1/133 |
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