Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria
Background: Iron-deficiency anemia (IDA) or iron deficiency (ID) is by far the most common form of disorder affecting the cognitive development, physical growth and school performance of children in developing countries including Nigeria. Objectives: In the present study, we aimed to examine whether...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019358748 |
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| author | Sani Sharif Usman Musa Dahiru Bashir Abdullahi Shariff Bilal Abdullahi Usman Muhammad Maigari Abdullahi Ibrahim Uba |
| author_facet | Sani Sharif Usman Musa Dahiru Bashir Abdullahi Shariff Bilal Abdullahi Usman Muhammad Maigari Abdullahi Ibrahim Uba |
| author_sort | Sani Sharif Usman |
| collection | DOAJ |
| description | Background: Iron-deficiency anemia (IDA) or iron deficiency (ID) is by far the most common form of disorder affecting the cognitive development, physical growth and school performance of children in developing countries including Nigeria. Objectives: In the present study, we aimed to examine whether IDA or ID, or both are associated with oxidative stress or otherwise by assessing the perturbations in oxidative stress markers including malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD). Methods: Here, a total of eighty-one IDA, ID, and healthy control subjects of twenty-seven replicates each, were recruited and investigated. Human serum MDA, CAT and SOD levels were quantitatively analyzed using Enzyme-Linked Immunosorbant Assay. Results: Mean serum MDA levels of IDA (5.10 ± 2.35 mmol/L) and ID (4.05 ± 1.35 mmol/L) groups were found to perturb significantly (p < 0.05), being higher than those of control (3.30 ± 0.95 mmol/L) subjects. Similarly, mean serum MDA levels of IDA (5.10 ± 2.35 mmol/L) group was found to be significantly (p < 0.05) higher when compared with ID (4.05 ± 1.35 mmol/L) subjects. Conversely, mean serum CAT and SOD activities of IDA (8.35 ± 2.21 ng/mL and 340.70 ± 153.65 ng/mL) group were found to differ significantly (p < 0.05), and those of ID (9.40 ± 1.47 ng/mL and 435.00 ± 144.75 ng/mL) subjects were found to perturb slightly (p > 0.05), being lower than those of control (10.40 ± 4.31 ng/mL and 482.12 ± 258.37 ng/mL) subjects. Conclusions: Taken together, the results of the present study showed that lipid peroxidation was dramatically increased in both IDA and ID subjects in hydroperoxide-superoxide-dependent manner; in contrast, enzymatic antioxidant capacity was drastically decreased in both IDA and ID groups as evidenced by biochemical markers. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T16:13:26Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-645546dcc92e46c0b07adc0c26015c6d2022-12-22T01:42:02ZengElsevierHeliyon2405-84402019-08-0158e02214Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, NigeriaSani Sharif Usman0Musa Dahiru1Bashir Abdullahi2Shariff Bilal Abdullahi3Usman Muhammad Maigari4Abdullahi Ibrahim Uba5Department of Biological Sciences, Faculty of Science, Federal University of Kashere, P.M.B. 0182, Gombe, Nigeria; Corresponding author.Department of Biological Sciences, Faculty of Science, Federal University of Kashere, P.M.B. 0182, Gombe, NigeriaDepartment of Educational Foundations, Faculty of Education, Federal University of Kashere, Gombe, NigeriaHealth Services Unit, Federal University of Kashere, Gombe, NigeriaChemical Pathology Department, Aminu Kano Teaching Hospital, Kano, NigeriaDepartment of Bioinformatics and Genetics, Graduate School of Science and Engineering, Kadir Has University, Istanbul, TurkeyBackground: Iron-deficiency anemia (IDA) or iron deficiency (ID) is by far the most common form of disorder affecting the cognitive development, physical growth and school performance of children in developing countries including Nigeria. Objectives: In the present study, we aimed to examine whether IDA or ID, or both are associated with oxidative stress or otherwise by assessing the perturbations in oxidative stress markers including malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD). Methods: Here, a total of eighty-one IDA, ID, and healthy control subjects of twenty-seven replicates each, were recruited and investigated. Human serum MDA, CAT and SOD levels were quantitatively analyzed using Enzyme-Linked Immunosorbant Assay. Results: Mean serum MDA levels of IDA (5.10 ± 2.35 mmol/L) and ID (4.05 ± 1.35 mmol/L) groups were found to perturb significantly (p < 0.05), being higher than those of control (3.30 ± 0.95 mmol/L) subjects. Similarly, mean serum MDA levels of IDA (5.10 ± 2.35 mmol/L) group was found to be significantly (p < 0.05) higher when compared with ID (4.05 ± 1.35 mmol/L) subjects. Conversely, mean serum CAT and SOD activities of IDA (8.35 ± 2.21 ng/mL and 340.70 ± 153.65 ng/mL) group were found to differ significantly (p < 0.05), and those of ID (9.40 ± 1.47 ng/mL and 435.00 ± 144.75 ng/mL) subjects were found to perturb slightly (p > 0.05), being lower than those of control (10.40 ± 4.31 ng/mL and 482.12 ± 258.37 ng/mL) subjects. Conclusions: Taken together, the results of the present study showed that lipid peroxidation was dramatically increased in both IDA and ID subjects in hydroperoxide-superoxide-dependent manner; in contrast, enzymatic antioxidant capacity was drastically decreased in both IDA and ID groups as evidenced by biochemical markers.http://www.sciencedirect.com/science/article/pii/S2405844019358748NutritionBiochemistryLipid peroxidationOxidative stressIron-deficiency anemiaBiochemical markers |
| spellingShingle | Sani Sharif Usman Musa Dahiru Bashir Abdullahi Shariff Bilal Abdullahi Usman Muhammad Maigari Abdullahi Ibrahim Uba Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria Heliyon Nutrition Biochemistry Lipid peroxidation Oxidative stress Iron-deficiency anemia Biochemical markers |
| title | Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria |
| title_full | Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria |
| title_fullStr | Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria |
| title_full_unstemmed | Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria |
| title_short | Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria |
| title_sort | status of malondialdehyde catalase and superoxide dismutase levels activities in schoolchildren with iron deficiency and iron deficiency anemia of kashere and its environs in gombe state nigeria |
| topic | Nutrition Biochemistry Lipid peroxidation Oxidative stress Iron-deficiency anemia Biochemical markers |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019358748 |
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