The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats
Background: Bone-related osteokines are crucial for bone function and metabolic response to physical activity. The present study aimed to shed light on the effect of different intensities of continuous and interval endurance training on the serum levels of some osteokines that are associated with wi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences and Health Services
2021-05-01
|
| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/article-1-426-en.html |
| _version_ | 1797973919140937728 |
|---|---|
| author | Mahnaz Shahbazi Nasser Behpoor Mohammad Faramarzi Ebrahim Banitalebi |
| author_facet | Mahnaz Shahbazi Nasser Behpoor Mohammad Faramarzi Ebrahim Banitalebi |
| author_sort | Mahnaz Shahbazi |
| collection | DOAJ |
| description | Background: Bone-related osteokines are crucial for bone function and metabolic response to physical activity. The present study aimed to shed light on the effect of different intensities of continuous and interval endurance training on the serum levels of some osteokines that are associated with wingless-related integration site (WNT) signaling pathway and Receptor Activator of Nuclear Factor (NF)-κB Ligand (RANKL) in old male Wistar rats.
Materials and Methods: A total of 24 old male Wistar rats (mean age: 23 months, mean weight: 437.93 g) were randomly assigned to three groups: interval endurance-training group (n=8), continuous endurance-training group (n=8), and control group (n=8). The continuous and interval training interventions comprised 8 weeks of treadmill exercise, 5 days a week. The continuous endurance-training group started to exercise at 60% of velocity at maximal oxygen uptake (vVO2max) for 16 min during the first week. It continued with 70% of vVO2max for 45 min from the fourth week onward. In contrast, the interval endurance-training group switched on exercise with 40%-80% of vVO2max from the first week and persisted with 30%-110% of vVO2max from the fourth week onward. As the exercise bout was completed, the enzyme-linked immunosorbent assay was applied to measure the study dependent variables. Statistical analysis was further performed using 1-way analysis of variance, considering the significance level of P≤0.05.
Results: The study results demonstrated a significant difference in the levels of Oteoprotegerin (OPG) (P=0.036) and RANKL (P=0.001) in the experimental (namely, interval and continuous training) groups compared with the controls following the exercise bout. However, the level of sclerostin was not significantly changed (P=0.549).
Conclusion: High-intensity endurance training in the RANKL/OPG and WNT pathways decreased sclerostin and RANKL levels, but this decrease was significant at the RANKL level. It was effective with regard to the intensities of different types of endurance exercise. |
| first_indexed | 2024-04-11T04:11:49Z |
| format | Article |
| id | doaj.art-6455e8ebc80a492ab4ffc4662b0f609d |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-04-11T04:11:49Z |
| publishDate | 2021-05-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-6455e8ebc80a492ab4ffc4662b0f609d2023-01-01T05:41:46ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2021-05-0192145154The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar RatsMahnaz Shahbazi0Nasser Behpoor1Mohammad Faramarzi2Ebrahim Banitalebi3 Department of Sport Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran. Department of Sport Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran. Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran. Department of Sport Sciences, Faculty of Sport Sciences, Shahrekord University, Shahrekord, Iran. Background: Bone-related osteokines are crucial for bone function and metabolic response to physical activity. The present study aimed to shed light on the effect of different intensities of continuous and interval endurance training on the serum levels of some osteokines that are associated with wingless-related integration site (WNT) signaling pathway and Receptor Activator of Nuclear Factor (NF)-κB Ligand (RANKL) in old male Wistar rats. Materials and Methods: A total of 24 old male Wistar rats (mean age: 23 months, mean weight: 437.93 g) were randomly assigned to three groups: interval endurance-training group (n=8), continuous endurance-training group (n=8), and control group (n=8). The continuous and interval training interventions comprised 8 weeks of treadmill exercise, 5 days a week. The continuous endurance-training group started to exercise at 60% of velocity at maximal oxygen uptake (vVO2max) for 16 min during the first week. It continued with 70% of vVO2max for 45 min from the fourth week onward. In contrast, the interval endurance-training group switched on exercise with 40%-80% of vVO2max from the first week and persisted with 30%-110% of vVO2max from the fourth week onward. As the exercise bout was completed, the enzyme-linked immunosorbent assay was applied to measure the study dependent variables. Statistical analysis was further performed using 1-way analysis of variance, considering the significance level of P≤0.05. Results: The study results demonstrated a significant difference in the levels of Oteoprotegerin (OPG) (P=0.036) and RANKL (P=0.001) in the experimental (namely, interval and continuous training) groups compared with the controls following the exercise bout. However, the level of sclerostin was not significantly changed (P=0.549). Conclusion: High-intensity endurance training in the RANKL/OPG and WNT pathways decreased sclerostin and RANKL levels, but this decrease was significant at the RANKL level. It was effective with regard to the intensities of different types of endurance exercise.http://rmm.mazums.ac.ir/article-1-426-en.htmlagingendurance trainingboneratwistar |
| spellingShingle | Mahnaz Shahbazi Nasser Behpoor Mohammad Faramarzi Ebrahim Banitalebi The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats Research in Molecular Medicine aging endurance training bone rat wistar |
| title | The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats |
| title_full | The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats |
| title_fullStr | The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats |
| title_full_unstemmed | The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats |
| title_short | The Effect of High- and Moderate-intensity Endurance Training on Some Anabolic/Catabolic Osteokines in Old Male Wistar Rats |
| title_sort | effect of high and moderate intensity endurance training on some anabolic catabolic osteokines in old male wistar rats |
| topic | aging endurance training bone rat wistar |
| url | http://rmm.mazums.ac.ir/article-1-426-en.html |
| work_keys_str_mv | AT mahnazshahbazi theeffectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT nasserbehpoor theeffectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT mohammadfaramarzi theeffectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT ebrahimbanitalebi theeffectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT mahnazshahbazi effectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT nasserbehpoor effectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT mohammadfaramarzi effectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats AT ebrahimbanitalebi effectofhighandmoderateintensityendurancetrainingonsomeanaboliccatabolicosteokinesinoldmalewistarrats |