Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein
The myelin sheath—a multi-double-bilayer membrane wrapped around axons—is an essential part of the nervous system which enables rapid signal conduction. Damage of this complex membrane system results in demyelinating diseases such as multiple sclerosis (MS). The process in which myelin is generated...
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.631277/full |
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| author | Benjamin Krugmann Benjamin Krugmann Alexandros Koutsioubas Luman Haris Luman Haris Samantha Micciulla Didier Lairez Aurel Radulescu Stephan Förster Andreas M. Stadler Andreas M. Stadler |
| author_facet | Benjamin Krugmann Benjamin Krugmann Alexandros Koutsioubas Luman Haris Luman Haris Samantha Micciulla Didier Lairez Aurel Radulescu Stephan Förster Andreas M. Stadler Andreas M. Stadler |
| author_sort | Benjamin Krugmann |
| collection | DOAJ |
| description | The myelin sheath—a multi-double-bilayer membrane wrapped around axons—is an essential part of the nervous system which enables rapid signal conduction. Damage of this complex membrane system results in demyelinating diseases such as multiple sclerosis (MS). The process in which myelin is generated in vivo is called myelination. In our study, we investigated the adhesion process of large unilamellar vesicles with a supported membrane bilayer that was coated with myelin basic protein (MBP) using time-resolved neutron reflectometry. Our aim was to mimic and to study the myelination process of membrane systems having either a lipid-composition resembling that of native myelin or that of the standard animal model for experimental autoimmune encephalomyelitis (EAE) which represents MS-like conditions. We were able to measure the kinetics of the partial formation of a double bilayer in those systems and to characterize the scattering length density profiles of the initial and final states of the membrane. The kinetics could be modeled using a random sequential adsorption simulation. By using a free energy minimization method, we were able to calculate the shape of the adhered vesicles and to determine the adhesion energy per MBP. For the native membrane the resulting adhesion energy per MBP is larger than that of the EAE modified membrane type. Our observations might help in understanding myelination and especially remyelination—a process in which damaged myelin is repaired—which is a promising candidate for treatment of the still mostly incurable demyelinating diseases such as MS. |
| first_indexed | 2024-12-16T23:56:48Z |
| format | Article |
| id | doaj.art-64591459a24c45af8db1c0d46cece7d5 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-16T23:56:48Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-64591459a24c45af8db1c0d46cece7d52022-12-21T22:11:12ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-05-01910.3389/fchem.2021.631277631277Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic ProteinBenjamin Krugmann0Benjamin Krugmann1Alexandros Koutsioubas2Luman Haris3Luman Haris4Samantha Micciulla5Didier Lairez6Aurel Radulescu7Stephan Förster8Andreas M. Stadler9Andreas M. Stadler10Jülich Centre for Neutron Science at MLZ, Forschungszentrum Jülich GmbH, Garching, GermanyInstitute of Physical Chemistry, RWTH Aachen University, Aachen, GermanyJülich Centre for Neutron Science at MLZ, Forschungszentrum Jülich GmbH, Garching, GermanyInstitute of Physical Chemistry, RWTH Aachen University, Aachen, GermanyJülich Centre for Neutron Science (JCNS-1) and Institute for Biological Information Processing (IBI-8), Forschungszentrum Jülich GmbH, Jülich, GermanyInstitut Laue-Langevin, Grenoble, FranceLaboratoire des Solides Irradiés, École Polytechnique, CEA, CNRS, Institut Polytechnique de Paris, Palaiseau, FranceJülich Centre for Neutron Science at MLZ, Forschungszentrum Jülich GmbH, Garching, GermanyJülich Centre for Neutron Science (JCNS-1) and Institute for Biological Information Processing (IBI-8), Forschungszentrum Jülich GmbH, Jülich, GermanyInstitute of Physical Chemistry, RWTH Aachen University, Aachen, GermanyJülich Centre for Neutron Science (JCNS-1) and Institute for Biological Information Processing (IBI-8), Forschungszentrum Jülich GmbH, Jülich, GermanyThe myelin sheath—a multi-double-bilayer membrane wrapped around axons—is an essential part of the nervous system which enables rapid signal conduction. Damage of this complex membrane system results in demyelinating diseases such as multiple sclerosis (MS). The process in which myelin is generated in vivo is called myelination. In our study, we investigated the adhesion process of large unilamellar vesicles with a supported membrane bilayer that was coated with myelin basic protein (MBP) using time-resolved neutron reflectometry. Our aim was to mimic and to study the myelination process of membrane systems having either a lipid-composition resembling that of native myelin or that of the standard animal model for experimental autoimmune encephalomyelitis (EAE) which represents MS-like conditions. We were able to measure the kinetics of the partial formation of a double bilayer in those systems and to characterize the scattering length density profiles of the initial and final states of the membrane. The kinetics could be modeled using a random sequential adsorption simulation. By using a free energy minimization method, we were able to calculate the shape of the adhered vesicles and to determine the adhesion energy per MBP. For the native membrane the resulting adhesion energy per MBP is larger than that of the EAE modified membrane type. Our observations might help in understanding myelination and especially remyelination—a process in which damaged myelin is repaired—which is a promising candidate for treatment of the still mostly incurable demyelinating diseases such as MS.https://www.frontiersin.org/articles/10.3389/fchem.2021.631277/fullneutron reflectometryadhesion energylipid membranesmyelin basic proteinvesicle fusionrandom sequential adsorption |
| spellingShingle | Benjamin Krugmann Benjamin Krugmann Alexandros Koutsioubas Luman Haris Luman Haris Samantha Micciulla Didier Lairez Aurel Radulescu Stephan Förster Andreas M. Stadler Andreas M. Stadler Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein Frontiers in Chemistry neutron reflectometry adhesion energy lipid membranes myelin basic protein vesicle fusion random sequential adsorption |
| title | Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein |
| title_full | Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein |
| title_fullStr | Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein |
| title_full_unstemmed | Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein |
| title_short | Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein |
| title_sort | adhesion process of biomimetic myelin membranes triggered by myelin basic protein |
| topic | neutron reflectometry adhesion energy lipid membranes myelin basic protein vesicle fusion random sequential adsorption |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.631277/full |
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