Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin
Introduction The aim of this prospective trial was to evaluate the ovarian reserve with anti-Müllerian hormone (AMH), which is the best predictor of ovarian reserve, and perform histological analysis after exposure to cisplatin with a GnRH agonist or antagonist. Material and methods Twenty-four Wis...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2019-08-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Evaluation-of-protective-effects-of-GnRH-agonist-or-antagonist-on-ovarian-reserve,111449,0,2.html |
| _version_ | 1797435381435596800 |
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| author | Mustafa Tas Gokalp Oner Pasa Ulug Adem Yavuz Bulent Ozcelik |
| author_facet | Mustafa Tas Gokalp Oner Pasa Ulug Adem Yavuz Bulent Ozcelik |
| author_sort | Mustafa Tas |
| collection | DOAJ |
| description | Introduction
The aim of this prospective trial was to evaluate the ovarian reserve with anti-Müllerian hormone (AMH), which is the best predictor of ovarian reserve, and perform histological analysis after exposure to cisplatin with a GnRH agonist or antagonist.
Material and methods
Twenty-four Wistar albino rats were randomly divided into three groups, each consisting of eight rats. In the GnRH agonist group (group 1), rats received a single dose of 50 mg/m2 cisplatin with 1 mg/kg triptorelin. In the GnRH antagonist group (group 2), rats received a single dose of 50 mg/m2 cisplatin with 1 mg/kg cetrorelix. In the control group (group 3), rats received 50 mg/m2 cisplatin. Ovarian reserve was assessed by AMH and histology.
Results
Primary follicle counts were higher in group 2 (4.50 ±1.47 vs. 3.50 ±1.70 vs. 3.00 ±3.54) and secondary follicle counts were higher in group 1 (2.96 ±1.11 vs. 1.74 ±1.03 vs. 1.37 ±3.11). Numbers of tertiary follicles were higher both in groups 1 and 2 than the control group (1.36 ±0.83 vs. 0.84 ±0.99 vs. 0.50 ±0.75). The total follicle count of the study groups were significantly higher compared with the control group (14.32 ±5.96 vs. 12.48 ±4.12 vs. 10.63 ±6.80). AMH was significantly higher in groups 1 and 2 compared with the control group (18.56 ±25.33 vs. 16.48 ±24.66 vs. 9.37 ±26.54).
Conclusions
This is the first prospective randomized controlled study showing the protective effects of GnRH agonist and antagonist on ovarian reserve after cisplatin exposure in an animal model. |
| first_indexed | 2024-03-09T10:47:23Z |
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| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:47:23Z |
| publishDate | 2019-08-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-64592e7a946e4bc5b941768d97b6a92e2023-12-01T09:52:59ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512019-08-0119244845110.5114/aoms.2019.87540111449Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatinMustafa Tas0Gokalp Oner1Pasa Ulug2Adem Yavuz3Bulent Ozcelik4Department of Obstetrics and Gynecology, Acıbadem Mehmet Ali Aydınlar University, Acıbadem Kayseri Hospital, Kayseri, TurkeyDepartment of Obstetrics and Gynecology, Magnet Hospital, Kayseri, TurkeyDepartment of Obstetrics and Gynecology, Ömer Halisdemir University, Niğde, TurkeyDepartment of Obstetrics and Gynecology, Erzincan University, Erzincan, TurkeyDepartment of Obstetrics and Gynecology, Erciyes University, Kayseri, TurkeyIntroduction The aim of this prospective trial was to evaluate the ovarian reserve with anti-Müllerian hormone (AMH), which is the best predictor of ovarian reserve, and perform histological analysis after exposure to cisplatin with a GnRH agonist or antagonist. Material and methods Twenty-four Wistar albino rats were randomly divided into three groups, each consisting of eight rats. In the GnRH agonist group (group 1), rats received a single dose of 50 mg/m2 cisplatin with 1 mg/kg triptorelin. In the GnRH antagonist group (group 2), rats received a single dose of 50 mg/m2 cisplatin with 1 mg/kg cetrorelix. In the control group (group 3), rats received 50 mg/m2 cisplatin. Ovarian reserve was assessed by AMH and histology. Results Primary follicle counts were higher in group 2 (4.50 ±1.47 vs. 3.50 ±1.70 vs. 3.00 ±3.54) and secondary follicle counts were higher in group 1 (2.96 ±1.11 vs. 1.74 ±1.03 vs. 1.37 ±3.11). Numbers of tertiary follicles were higher both in groups 1 and 2 than the control group (1.36 ±0.83 vs. 0.84 ±0.99 vs. 0.50 ±0.75). The total follicle count of the study groups were significantly higher compared with the control group (14.32 ±5.96 vs. 12.48 ±4.12 vs. 10.63 ±6.80). AMH was significantly higher in groups 1 and 2 compared with the control group (18.56 ±25.33 vs. 16.48 ±24.66 vs. 9.37 ±26.54). Conclusions This is the first prospective randomized controlled study showing the protective effects of GnRH agonist and antagonist on ovarian reserve after cisplatin exposure in an animal model.https://www.archivesofmedicalscience.com/Evaluation-of-protective-effects-of-GnRH-agonist-or-antagonist-on-ovarian-reserve,111449,0,2.htmlovarian reservecisplatinanti-müllerian hormone |
| spellingShingle | Mustafa Tas Gokalp Oner Pasa Ulug Adem Yavuz Bulent Ozcelik Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin Archives of Medical Science ovarian reserve cisplatin anti-müllerian hormone |
| title | Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin |
| title_full | Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin |
| title_fullStr | Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin |
| title_full_unstemmed | Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin |
| title_short | Evaluation of protective effects of GnRH agonist or antagonist on ovarian reserve with anti-Müllerian hormone and histological analysis in a rat model using cisplatin |
| title_sort | evaluation of protective effects of gnrh agonist or antagonist on ovarian reserve with anti mullerian hormone and histological analysis in a rat model using cisplatin |
| topic | ovarian reserve cisplatin anti-müllerian hormone |
| url | https://www.archivesofmedicalscience.com/Evaluation-of-protective-effects-of-GnRH-agonist-or-antagonist-on-ovarian-reserve,111449,0,2.html |
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