Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues
The importance of the ovarian extracellular environment and tissue rigidity on follicle survival and development has gained attention in recent years. Our laboratory has anecdotally observed differences in the rigidity of domestic cat and dog ovarian cortical tissues, which have been postulated to u...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/11/1285 |
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| author | Jennifer B. Nagashima Shoshana Zenilman April Raab Helim Aranda-Espinoza Nucharin Songsasen |
| author_facet | Jennifer B. Nagashima Shoshana Zenilman April Raab Helim Aranda-Espinoza Nucharin Songsasen |
| author_sort | Jennifer B. Nagashima |
| collection | DOAJ |
| description | The importance of the ovarian extracellular environment and tissue rigidity on follicle survival and development has gained attention in recent years. Our laboratory has anecdotally observed differences in the rigidity of domestic cat and dog ovarian cortical tissues, which have been postulated to underlie the differences in in vitro culture responses between the species, wherein cat ovarian tissues display higher survival in extended incubation. Here, the tensile strengths of cat and dog ovarian cortical tissues were compared via micropipette aspiration. The underlying collagen patterns, including fiber length, thickness, alignment, curvature, branch points and end points, and overall tissue lacunary and high-density matrix (HDM) were quantified via picrosirius red staining and TWOMBLI analysis. Finally, we explored the potential of MMP (−1 and −9) and TIMP1 supplementation in modulating tissue rigidity, collagen structure, and follicle activation in vitro. No differences in stiffness were observed between cat or dog cortical tissues, or pre- versus post-pubertal status. Cat ovarian collagen was characterized by an increased number of branch points, thinner fibers, and lower HDM compared with dog ovarian collagen, and cat tissues exposed to MMP9 in vitro displayed a reduced Young’s modulus. Yet, MMP exposure had a minor impact on follicle development in vitro in either species. This study contributes to our growing understanding of the interactions among the physical properties of the ovarian microenvironment, collagen patterns, and follicle development in vitro. |
| first_indexed | 2024-03-09T17:00:35Z |
| format | Article |
| id | doaj.art-645d6b2489f6458591c3d3369584dee6 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:00:35Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-645d6b2489f6458591c3d3369584dee62023-11-24T14:29:49ZengMDPI AGBioengineering2306-53542023-11-011011128510.3390/bioengineering10111285Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical TissuesJennifer B. Nagashima0Shoshana Zenilman1April Raab2Helim Aranda-Espinoza3Nucharin Songsasen4Center for Species Survival, Smithsonian National Zoo and Conservation Biology Institute, 1500 Remount Rd., Front Royal, VA 22630, USACollege of Veterinary Medicine, Cornell University, 144 East Ave, Ithaca, NY 14850, USACollege of Veterinary Medicine, Michigan State University, 784 Wilson Rd., East Lansing, MI 48824, USAFischell Department of Bioengineering, University of Maryland, 3108 A. James Clark Hall, College Park, MD 20742, USACenter for Species Survival, Smithsonian National Zoo and Conservation Biology Institute, 1500 Remount Rd., Front Royal, VA 22630, USAThe importance of the ovarian extracellular environment and tissue rigidity on follicle survival and development has gained attention in recent years. Our laboratory has anecdotally observed differences in the rigidity of domestic cat and dog ovarian cortical tissues, which have been postulated to underlie the differences in in vitro culture responses between the species, wherein cat ovarian tissues display higher survival in extended incubation. Here, the tensile strengths of cat and dog ovarian cortical tissues were compared via micropipette aspiration. The underlying collagen patterns, including fiber length, thickness, alignment, curvature, branch points and end points, and overall tissue lacunary and high-density matrix (HDM) were quantified via picrosirius red staining and TWOMBLI analysis. Finally, we explored the potential of MMP (−1 and −9) and TIMP1 supplementation in modulating tissue rigidity, collagen structure, and follicle activation in vitro. No differences in stiffness were observed between cat or dog cortical tissues, or pre- versus post-pubertal status. Cat ovarian collagen was characterized by an increased number of branch points, thinner fibers, and lower HDM compared with dog ovarian collagen, and cat tissues exposed to MMP9 in vitro displayed a reduced Young’s modulus. Yet, MMP exposure had a minor impact on follicle development in vitro in either species. This study contributes to our growing understanding of the interactions among the physical properties of the ovarian microenvironment, collagen patterns, and follicle development in vitro.https://www.mdpi.com/2306-5354/10/11/1285ovarydomestic catdomestic dogrigiditycollagenmatrix metalloproteinase |
| spellingShingle | Jennifer B. Nagashima Shoshana Zenilman April Raab Helim Aranda-Espinoza Nucharin Songsasen Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues Bioengineering ovary domestic cat domestic dog rigidity collagen matrix metalloproteinase |
| title | Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues |
| title_full | Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues |
| title_fullStr | Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues |
| title_full_unstemmed | Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues |
| title_short | Comparative Tensile Properties and Collagen Patterns in Domestic Cat (<i>Felis catus</i>) and Dog (<i>Canis lupus familiaris</i>) Ovarian Cortical Tissues |
| title_sort | comparative tensile properties and collagen patterns in domestic cat i felis catus i and dog i canis lupus familiaris i ovarian cortical tissues |
| topic | ovary domestic cat domestic dog rigidity collagen matrix metalloproteinase |
| url | https://www.mdpi.com/2306-5354/10/11/1285 |
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