Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders
Objective: To determine the concordance among clinical, histopathological and immunofluorescence as diagnostic methods for intraepidermal immunobullous disorders. Method: The prospective cross-sectional study was conducted at the Institute of Skin Diseases, Karachi, from December 2020 to December...
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| Format: | Article |
| Language: | English |
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Pakistan Medical Association
2024-02-01
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| Series: | Journal of the Pakistan Medical Association |
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| Online Access: | https://ojs.jpma.org.pk/index.php/public_html/article/view/9509 |
| _version_ | 1797296140277776384 |
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| author | Erum Ashraf Faryal Shareef |
| author_facet | Erum Ashraf Faryal Shareef |
| author_sort | Erum Ashraf |
| collection | DOAJ |
| description |
Objective: To determine the concordance among clinical, histopathological and immunofluorescence as diagnostic methods for intraepidermal immunobullous disorders.
Method: The prospective cross-sectional study was conducted at the Institute of Skin Diseases, Karachi, from December 2020 to December 2022, and comprised adult patients of either gender presenting with complaints of bullae, vesicles, pustules and crusts on the skin or mucous membrane. Diagnostic findings of each patient as obtained by clinical assessment, microscopy and direct immunofluorescence were compared. Data was analysed using SPSS 19.
Results: Of the 81 patients, 41(50.6%) were males and 40(49.4%) were females. The overall median age was 35 years (interquartile range: 23 years), with 66(75%) patients aged 19-55 years. The predominant body site involved was the trunk 49(60.5%), followed by mucosa 26(32.1%). Clinical diagnosis detected 80(98.7%) cases, compared to 76(93.8%) by microscopy and 81(100%) by direct immunofluorescence.
Conclusion: Direct immunofluorescence was found to be the gold standard for a confirmatory diagnosis of intraepidermal immunobullous disorders, especially when clinical and histopathology findings were inconclusive.
Key Words: Intraepidermal immunobullous disorders, Pemphigus vulgaris, Histopathology, Direct immunofluorescence.
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| first_indexed | 2024-03-07T21:59:04Z |
| format | Article |
| id | doaj.art-645f171c920045fa93c4b9abdb7e8481 |
| institution | Directory Open Access Journal |
| issn | 0030-9982 |
| language | English |
| last_indexed | 2024-03-07T21:59:04Z |
| publishDate | 2024-02-01 |
| publisher | Pakistan Medical Association |
| record_format | Article |
| series | Journal of the Pakistan Medical Association |
| spelling | doaj.art-645f171c920045fa93c4b9abdb7e84812024-02-24T05:33:36ZengPakistan Medical AssociationJournal of the Pakistan Medical Association0030-99822024-02-0174410.47391/JPMA.9509Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disordersErum Ashraf0Faryal Shareef1Department of Dermatology, Institute of Skin Diseases, Karachi, PakistanDepartment of Dermatology, Institute of Skin Diseases, Karachi, Pakistan Objective: To determine the concordance among clinical, histopathological and immunofluorescence as diagnostic methods for intraepidermal immunobullous disorders. Method: The prospective cross-sectional study was conducted at the Institute of Skin Diseases, Karachi, from December 2020 to December 2022, and comprised adult patients of either gender presenting with complaints of bullae, vesicles, pustules and crusts on the skin or mucous membrane. Diagnostic findings of each patient as obtained by clinical assessment, microscopy and direct immunofluorescence were compared. Data was analysed using SPSS 19. Results: Of the 81 patients, 41(50.6%) were males and 40(49.4%) were females. The overall median age was 35 years (interquartile range: 23 years), with 66(75%) patients aged 19-55 years. The predominant body site involved was the trunk 49(60.5%), followed by mucosa 26(32.1%). Clinical diagnosis detected 80(98.7%) cases, compared to 76(93.8%) by microscopy and 81(100%) by direct immunofluorescence. Conclusion: Direct immunofluorescence was found to be the gold standard for a confirmatory diagnosis of intraepidermal immunobullous disorders, especially when clinical and histopathology findings were inconclusive. Key Words: Intraepidermal immunobullous disorders, Pemphigus vulgaris, Histopathology, Direct immunofluorescence. https://ojs.jpma.org.pk/index.php/public_html/article/view/9509Intraepidermal immunobullous disordersPemphigus vulgarisHistopathologyDirect immunofluorescence |
| spellingShingle | Erum Ashraf Faryal Shareef Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders Journal of the Pakistan Medical Association Intraepidermal immunobullous disorders Pemphigus vulgaris Histopathology Direct immunofluorescence |
| title | Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| title_full | Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| title_fullStr | Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| title_full_unstemmed | Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| title_short | Concordance of clinical, histopathologic and direct Immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| title_sort | concordance of clinical histopathologic and direct immunofluorescence findings in patients with intraepidermal immunobullous disorders |
| topic | Intraepidermal immunobullous disorders Pemphigus vulgaris Histopathology Direct immunofluorescence |
| url | https://ojs.jpma.org.pk/index.php/public_html/article/view/9509 |
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