The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy
Background: Diabetic neuropathy (DN) is a prevalent complication of diabetes mellitus characterized by pain and inflammation. Long non-coding RNAs (lncRNAs) have been associated with DN. This study aimed to investigate transcript levels of Metastasis-associated lung adenocarcinoma transcript 1 (MALA...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022004662 |
| _version_ | 1818327800112742400 |
|---|---|
| author | Donya Ashjari Negin Karamali Misagh Rajabinejad Seyedeh Sara Hassani Leila Afshar Hezarkhani Daryoush Afshari Ali Gorgin Karaji Farhad Salari Alireza Rezaiemanesh |
| author_facet | Donya Ashjari Negin Karamali Misagh Rajabinejad Seyedeh Sara Hassani Leila Afshar Hezarkhani Daryoush Afshari Ali Gorgin Karaji Farhad Salari Alireza Rezaiemanesh |
| author_sort | Donya Ashjari |
| collection | DOAJ |
| description | Background: Diabetic neuropathy (DN) is a prevalent complication of diabetes mellitus characterized by pain and inflammation. Long non-coding RNAs (lncRNAs) have been associated with DN. This study aimed to investigate transcript levels of Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), microRNA (miR)-1-3p, and C-X-C motif chemokine receptor 4 (CXCR4) in the DN patients and type 2 diabetes mellitus (T2DM) cases without neuropathy. Methods: Here, 20 cases with DN and 20 T2DM subjects without neuropathy (as the control group) were included. Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of all participants. The expression levels of targets were evaluated by Real-time-PCR. Results: Results showed that MALAT1 (Fold change = 2.47, P = 0.03) and CXCR4 (Fold change = 1.65, P = 0.023) were significantly upregulated, while miR-1-3p was downregulated (Fold change = 0.9, P = 0.028) in whole blood samples from DN patients compared to the control group. A significant correlation was found between transcript levels of MALAT1 and CXCR4 (rho = 0.84; P < 0.0001). Conclusions: This study suggests a possible involvement of the MALAT1/miR-1-3p/CXCR4 axis in the pathogenesis of DN. |
| first_indexed | 2024-12-13T12:22:01Z |
| format | Article |
| id | doaj.art-645f9e89f15047aaae855fe9adc0bf34 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-13T12:22:01Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-645f9e89f15047aaae855fe9adc0bf342022-12-21T23:46:33ZengElsevierHeliyon2405-84402022-03-0183e09178The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathyDonya Ashjari0Negin Karamali1Misagh Rajabinejad2Seyedeh Sara Hassani3Leila Afshar Hezarkhani4Daryoush Afshari5Ali Gorgin Karaji6Farhad Salari7Alireza Rezaiemanesh8Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranStudent Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Neurology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Neurology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author.Background: Diabetic neuropathy (DN) is a prevalent complication of diabetes mellitus characterized by pain and inflammation. Long non-coding RNAs (lncRNAs) have been associated with DN. This study aimed to investigate transcript levels of Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), microRNA (miR)-1-3p, and C-X-C motif chemokine receptor 4 (CXCR4) in the DN patients and type 2 diabetes mellitus (T2DM) cases without neuropathy. Methods: Here, 20 cases with DN and 20 T2DM subjects without neuropathy (as the control group) were included. Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of all participants. The expression levels of targets were evaluated by Real-time-PCR. Results: Results showed that MALAT1 (Fold change = 2.47, P = 0.03) and CXCR4 (Fold change = 1.65, P = 0.023) were significantly upregulated, while miR-1-3p was downregulated (Fold change = 0.9, P = 0.028) in whole blood samples from DN patients compared to the control group. A significant correlation was found between transcript levels of MALAT1 and CXCR4 (rho = 0.84; P < 0.0001). Conclusions: This study suggests a possible involvement of the MALAT1/miR-1-3p/CXCR4 axis in the pathogenesis of DN.http://www.sciencedirect.com/science/article/pii/S2405844022004662Diabetic neuropathyMALAT1miR-1-3pCXCR4 |
| spellingShingle | Donya Ashjari Negin Karamali Misagh Rajabinejad Seyedeh Sara Hassani Leila Afshar Hezarkhani Daryoush Afshari Ali Gorgin Karaji Farhad Salari Alireza Rezaiemanesh The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy Heliyon Diabetic neuropathy MALAT1 miR-1-3p CXCR4 |
| title | The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy |
| title_full | The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy |
| title_fullStr | The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy |
| title_full_unstemmed | The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy |
| title_short | The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy |
| title_sort | axis of long non coding rna malat1 mir 1 3p cxcr4 is dysregulated in patients with diabetic neuropathy |
| topic | Diabetic neuropathy MALAT1 miR-1-3p CXCR4 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022004662 |
| work_keys_str_mv | AT donyaashjari theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT neginkaramali theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT misaghrajabinejad theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT seyedehsarahassani theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT leilaafsharhezarkhani theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT daryoushafshari theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT aligorginkaraji theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT farhadsalari theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT alirezarezaiemanesh theaxisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT donyaashjari axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT neginkaramali axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT misaghrajabinejad axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT seyedehsarahassani axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT leilaafsharhezarkhani axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT daryoushafshari axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT aligorginkaraji axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT farhadsalari axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy AT alirezarezaiemanesh axisoflongnoncodingrnamalat1mir13pcxcr4isdysregulatedinpatientswithdiabeticneuropathy |