Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects
Insect metabolites play vital roles in regulating the physiology, behavior, and numerous adaptations of insects, which has contributed to them becoming the largest class of Animalia. However, systematic metabolomics within the insects is still unclear. The present study performed a widely targeted m...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/13/6/735 |
| _version_ | 1797593513745973248 |
|---|---|
| author | Zhaoxin Li Yunlong Cheng Jinxin Chen Weijun Xu Wentao Ma Sheng Li Erxia Du |
| author_facet | Zhaoxin Li Yunlong Cheng Jinxin Chen Weijun Xu Wentao Ma Sheng Li Erxia Du |
| author_sort | Zhaoxin Li |
| collection | DOAJ |
| description | Insect metabolites play vital roles in regulating the physiology, behavior, and numerous adaptations of insects, which has contributed to them becoming the largest class of Animalia. However, systematic metabolomics within the insects is still unclear. The present study performed a widely targeted metabolomics analysis based on the HPLC-MS/MS technology to construct a novel integrated metabolic database presenting comprehensive multimetabolite profiles from nine insect species across three metamorphosis types. A total of 1442 metabolites were identified, including amino acids and their metabolites, organic acids and their derivatives, fatty acids (FAs), glycerophospholipids (GPs), nucleotides and their metabolites, and benzene and its substituted derivatives. Among them, 622 metabolites were used to generate a 0 and 1 matrix based on their presence or absence, and these metabolites were enriched in arachidonic acid metabolism, tyrosine metabolism, phenylalanine metabolism, and insect hormone biosynthesis pathways. Our study revealed that there is a high coincidence between the evolutionary relationships of the species and the hierarchical cluster based on the types of metabolites, while the quantities of the metabolites show a high diversity among species. The metabolome of the nine representative insects provides an important platform for implementing the analysis of insect systemic metabolites and biological events at the metabolic level. |
| first_indexed | 2024-03-11T02:10:22Z |
| format | Article |
| id | doaj.art-6462f457561a411ab0abdcb1d1d0f9fb |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T02:10:22Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6462f457561a411ab0abdcb1d1d0f9fb2023-11-18T11:34:58ZengMDPI AGMetabolites2218-19892023-06-0113673510.3390/metabo13060735Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in InsectsZhaoxin Li0Yunlong Cheng1Jinxin Chen2Weijun Xu3Wentao Ma4Sheng Li5Erxia Du6Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaInsect metabolites play vital roles in regulating the physiology, behavior, and numerous adaptations of insects, which has contributed to them becoming the largest class of Animalia. However, systematic metabolomics within the insects is still unclear. The present study performed a widely targeted metabolomics analysis based on the HPLC-MS/MS technology to construct a novel integrated metabolic database presenting comprehensive multimetabolite profiles from nine insect species across three metamorphosis types. A total of 1442 metabolites were identified, including amino acids and their metabolites, organic acids and their derivatives, fatty acids (FAs), glycerophospholipids (GPs), nucleotides and their metabolites, and benzene and its substituted derivatives. Among them, 622 metabolites were used to generate a 0 and 1 matrix based on their presence or absence, and these metabolites were enriched in arachidonic acid metabolism, tyrosine metabolism, phenylalanine metabolism, and insect hormone biosynthesis pathways. Our study revealed that there is a high coincidence between the evolutionary relationships of the species and the hierarchical cluster based on the types of metabolites, while the quantities of the metabolites show a high diversity among species. The metabolome of the nine representative insects provides an important platform for implementing the analysis of insect systemic metabolites and biological events at the metabolic level.https://www.mdpi.com/2218-1989/13/6/735comparative metabolomicsmetabolitesLC–MS/MSinsects |
| spellingShingle | Zhaoxin Li Yunlong Cheng Jinxin Chen Weijun Xu Wentao Ma Sheng Li Erxia Du Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects Metabolites comparative metabolomics metabolites LC–MS/MS insects |
| title | Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects |
| title_full | Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects |
| title_fullStr | Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects |
| title_full_unstemmed | Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects |
| title_short | Widely Targeted HPLC-MS/MS Metabolomics Analysis Reveals Natural Metabolic Insights in Insects |
| title_sort | widely targeted hplc ms ms metabolomics analysis reveals natural metabolic insights in insects |
| topic | comparative metabolomics metabolites LC–MS/MS insects |
| url | https://www.mdpi.com/2218-1989/13/6/735 |
| work_keys_str_mv | AT zhaoxinli widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT yunlongcheng widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT jinxinchen widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT weijunxu widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT wentaoma widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT shengli widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects AT erxiadu widelytargetedhplcmsmsmetabolomicsanalysisrevealsnaturalmetabolicinsightsininsects |