Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production

Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production

In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous indigoidine production was examined using three distinct omic datasets. Transcriptomic data indicated that CRISPR/dCpf1-interference (CRISPRi) mediated multiplex repression caused global gene expression changes, implyi...

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Bibliographic Details
Main Authors:	Jeffrey J. Czajka, Deepanwita Banerjee, Thomas Eng, Javier Menasalvas, Chunsheng Yan, Nathalie Munoz Munoz, Brenton C. Poirier, Young-Mo Kim, Scott E. Baker, Yinjie J. Tang, Aindrila Mukhopadhyay
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Series:	Metabolic Engineering Communications
Subjects:	Growth coupling 13C-MFA Pseudomonas putida KT2440 Multiplexed CRISPR interference Indigoidine Cpf1/Cas12a
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214030122000153

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