Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts
The mechanisms of ovarian endometrioid cyst formation, or cystic ovarian endometriosis, still remain to be elucidated. To address this issue, we analyzed the involvement of mast cell (MC) tryptase and carboxypeptidase A3 (CPA3) in the development of endometriomas. It was found that the formation of...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6498 |
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| author | Dmitri Atiakshin Olga Patsap Andrey Kostin Lyudmila Mikhalyova Igor Buchwalow Markus Tiemann |
| author_facet | Dmitri Atiakshin Olga Patsap Andrey Kostin Lyudmila Mikhalyova Igor Buchwalow Markus Tiemann |
| author_sort | Dmitri Atiakshin |
| collection | DOAJ |
| description | The mechanisms of ovarian endometrioid cyst formation, or cystic ovarian endometriosis, still remain to be elucidated. To address this issue, we analyzed the involvement of mast cell (MC) tryptase and carboxypeptidase A3 (CPA3) in the development of endometriomas. It was found that the formation of endometrioid cysts was accompanied by an increased MC population in the ovarian medulla, as well as by an MC appearance in the cortical substance. The formation of MC subpopulations was associated with endometrioma wall structures. An active, targeted secretion of tryptase and CPA3 to the epithelium of endometrioid cysts, immunocompetent cells, and the cells of the cytogenic ovarian stroma was detected. The identification of specific proteases in the cell nuclei of the ovarian local tissue microenvironment suggests new mechanisms for the regulatory effects of MCs. The cytoplasmic outgrowths of MCs propagate in the structures of the stroma over a considerable distance; they offer new potentials for MC effects on the structures of the ovarian-specific tissue microenvironment under pathological conditions. Our findings indicate the potential roles of MC tryptase and CPA3 in the development of ovarian endometriomas and infer new perspectives on their uses as pharmacological targets in personalized medicine. |
| first_indexed | 2024-03-11T05:34:51Z |
| format | Article |
| id | doaj.art-646503bc8234463a85376f0befefe326 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:34:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-646503bc8234463a85376f0befefe3262023-11-17T16:51:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247649810.3390/ijms24076498Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid CystsDmitri Atiakshin0Olga Patsap1Andrey Kostin2Lyudmila Mikhalyova3Igor Buchwalow4Markus Tiemann5Research and Educational Resource Centre for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples’ Friendship University of Russia, 117198 Moscow, RussiaResearch and Educational Resource Centre for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples’ Friendship University of Russia, 117198 Moscow, RussiaResearch and Educational Resource Centre for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples’ Friendship University of Russia, 117198 Moscow, RussiaAvtsyn Research Institute of Human Morphology, 117418 Moscow, RussiaResearch and Educational Resource Centre for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples’ Friendship University of Russia, 117198 Moscow, RussiaInstitute for Hematopathology, 22547 Hamburg, GermanyThe mechanisms of ovarian endometrioid cyst formation, or cystic ovarian endometriosis, still remain to be elucidated. To address this issue, we analyzed the involvement of mast cell (MC) tryptase and carboxypeptidase A3 (CPA3) in the development of endometriomas. It was found that the formation of endometrioid cysts was accompanied by an increased MC population in the ovarian medulla, as well as by an MC appearance in the cortical substance. The formation of MC subpopulations was associated with endometrioma wall structures. An active, targeted secretion of tryptase and CPA3 to the epithelium of endometrioid cysts, immunocompetent cells, and the cells of the cytogenic ovarian stroma was detected. The identification of specific proteases in the cell nuclei of the ovarian local tissue microenvironment suggests new mechanisms for the regulatory effects of MCs. The cytoplasmic outgrowths of MCs propagate in the structures of the stroma over a considerable distance; they offer new potentials for MC effects on the structures of the ovarian-specific tissue microenvironment under pathological conditions. Our findings indicate the potential roles of MC tryptase and CPA3 in the development of ovarian endometriomas and infer new perspectives on their uses as pharmacological targets in personalized medicine.https://www.mdpi.com/1422-0067/24/7/6498tryptasecarboxypeptidase A3mast cellsovarian endometriomaspecific tissue microenvironment |
| spellingShingle | Dmitri Atiakshin Olga Patsap Andrey Kostin Lyudmila Mikhalyova Igor Buchwalow Markus Tiemann Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts International Journal of Molecular Sciences tryptase carboxypeptidase A3 mast cells ovarian endometrioma specific tissue microenvironment |
| title | Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts |
| title_full | Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts |
| title_fullStr | Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts |
| title_full_unstemmed | Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts |
| title_short | Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts |
| title_sort | mast cell tryptase and carboxypeptidase a3 in the formation of ovarian endometrioid cysts |
| topic | tryptase carboxypeptidase A3 mast cells ovarian endometrioma specific tissue microenvironment |
| url | https://www.mdpi.com/1422-0067/24/7/6498 |
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