A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences
Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously ide...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018364879 |
| _version_ | 1818280519504232448 |
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| author | Gloria Valentine Nakato Emmanuel Wicker Teresa A. Coutinho George Mahuku David J. Studholme |
| author_facet | Gloria Valentine Nakato Emmanuel Wicker Teresa A. Coutinho George Mahuku David J. Studholme |
| author_sort | Gloria Valentine Nakato |
| collection | DOAJ |
| description | Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains. |
| first_indexed | 2024-12-12T23:50:31Z |
| format | Article |
| id | doaj.art-6469f6c9703945618d548d213afa6c4d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-12T23:50:31Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-6469f6c9703945618d548d213afa6c4d2022-12-22T00:06:43ZengElsevierHeliyon2405-84402018-12-01412e01080A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequencesGloria Valentine Nakato0Emmanuel Wicker1Teresa A. Coutinho2George Mahuku3David J. Studholme4Pathology, International Institute of Tropical Agriculture, P.O. Box 7878, Kampala, Uganda; Department of Microbiology and Plant Pathology, Centre for Microbial Ecology and Genomics (CMEG), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X28, Pretoria 0028, South AfricaUMR IPME, Univ Montpellier, CIRAD, IRD, Montpellier, France; CIRAD, UMR “Interactions Plantes-Microorganismes-Environnement”(IPME), 911, Avenue Agropolis, BP 64501, F-34394 Montpellier Cedex 5, France; Corresponding author.Department of Microbiology and Plant Pathology, Centre for Microbial Ecology and Genomics (CMEG), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X28, Pretoria 0028, South AfricaPathology, International Institute of Tropical Agriculture, P.O. Box 7878, Kampala, Uganda; International Institute of Tropical Agriculture (IITA), P.O. Box, 34443, Dar es Salaam, TanzaniaBiosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, United Kingdom; Corresponding author.Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains.http://www.sciencedirect.com/science/article/pii/S2405844018364879BioinformaticsMicrobiologyMolecular biologyPlant biology |
| spellingShingle | Gloria Valentine Nakato Emmanuel Wicker Teresa A. Coutinho George Mahuku David J. Studholme A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences Heliyon Bioinformatics Microbiology Molecular biology Plant biology |
| title | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences |
| title_full | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences |
| title_fullStr | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences |
| title_full_unstemmed | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences |
| title_short | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences |
| title_sort | highly specific tool for identification of xanthomonas vasicola pv musacearum based on five xvm specific coding sequences |
| topic | Bioinformatics Microbiology Molecular biology Plant biology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018364879 |
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