Interpretative optimization of the isocratic ion chromatographic separation of anions
Interpretive retention modeling was utilized to optimize the isocratic ion chromatographic (IC) separation of the nine anions (formate, fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate, oxalate). The carbonate-bicarbonate eluent was used and separation was done on a Dionex...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Serbian Chemical Society
2016-01-01
|
| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2016/0352-51391600022T.pdf |
| Summary: | Interpretive retention modeling was utilized to optimize the isocratic ion
chromatographic (IC) separation of the nine anions (formate, fluoride,
chloride, nitrite, bromide, nitrate, phosphate, sulfate, oxalate). The
carbonate-bicarbonate eluent was used and separation was done on a Dionex
AS14 ion-exchange column. The influence of combined effects of two mobile
phase factors, the total eluent concentration (2 - 6 mM) and the
carbonate/bicaronate ratio from 1:9 to 9:1 (which corespondent to pH range
9.35 - 11.27), on the IC separation was studied. The multiple species
analyte/eluent model that takes into account ion-exchange equilibria of the
eluent and sample anions was used. In order to estimate the parameters in the
model, a non-linear fitting of the retention data, obtained at two-factor
three-level experimental design, was applied. To find the optimal conditions
in the experimental design, the normalized resolution product as a
chromatographic objective function was employed. This criterion includes both
the individual peak resolution and the total analysis time. A good agreement
between experimental and simulated chromatograms was obtained. [Projekat
Ministarstva nauke Republike Srbije, br. III43009] |
|---|---|
| ISSN: | 0352-5139 1820-7421 |