Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering
Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/9/1046 |
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| author | Darja Božič Simona Sitar Ita Junkar Roman Štukelj Manca Pajnič Ema Žagar Veronika Kralj-Iglič Ksenija Kogej |
| author_facet | Darja Božič Simona Sitar Ita Junkar Roman Štukelj Manca Pajnič Ema Žagar Veronika Kralj-Iglič Ksenija Kogej |
| author_sort | Darja Božič |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be <i>ρ </i>~ 1 (0.94−1.1 in exosome standards and 0.7−1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity. |
| first_indexed | 2024-03-12T19:33:28Z |
| format | Article |
| id | doaj.art-6471b960c91d45b2bb42d5ca7e5c9772 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:33:28Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-6471b960c91d45b2bb42d5ca7e5c97722023-08-02T04:22:08ZengMDPI AGCells2073-44092019-09-0189104610.3390/cells8091046cells8091046Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light ScatteringDarja Božič0Simona Sitar1Ita Junkar2Roman Štukelj3Manca Pajnič4Ema Žagar5Veronika Kralj-Iglič6Ksenija Kogej7Department of Chemistry and Biochemistry, University of Ljubljana, Faculty of Chemistry and Chemical Technology, SI-1000 Ljubljana, SloveniaDepartment of Polymer Chemistry and Technology, National Institute of Chemistry, SI-1001 Ljubljana, SloveniaDepartment of Surface Engineering and Optoelectronics, Josef Stefan Institute, SI-1000 Ljubljana, SloveniaBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, SloveniaBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, SloveniaDepartment of Polymer Chemistry and Technology, National Institute of Chemistry, SI-1001 Ljubljana, SloveniaBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, SloveniaDepartment of Chemistry and Biochemistry, University of Ljubljana, Faculty of Chemistry and Chemical Technology, SI-1000 Ljubljana, SloveniaExtracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be <i>ρ </i>~ 1 (0.94−1.1 in exosome standards and 0.7−1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.https://www.mdpi.com/2073-4409/8/9/1046extracellular vesiclesexosomesblood plasmadynamic light scatteringstatic light scatteringshape characterizationviscosity of blood plasma |
| spellingShingle | Darja Božič Simona Sitar Ita Junkar Roman Štukelj Manca Pajnič Ema Žagar Veronika Kralj-Iglič Ksenija Kogej Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering Cells extracellular vesicles exosomes blood plasma dynamic light scattering static light scattering shape characterization viscosity of blood plasma |
| title | Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering |
| title_full | Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering |
| title_fullStr | Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering |
| title_full_unstemmed | Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering |
| title_short | Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering |
| title_sort | viscosity of plasma as a key factor in assessment of extracellular vesicles by light scattering |
| topic | extracellular vesicles exosomes blood plasma dynamic light scattering static light scattering shape characterization viscosity of blood plasma |
| url | https://www.mdpi.com/2073-4409/8/9/1046 |
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