Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data

Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data

Abstract Background Quantitative real-time reverse transcription-polymerase chain reaction has been widely used in gene expression analysis, however, to have reliable and accurate results, reference genes are necessary to normalize gene expression under different experimental conditions. Several rel...

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Bibliographic Details
Main Authors: Wenxian Liang, Xiaoxing Zou, Rebeca Carballar-Lejarazú, Lingjiao Wu, Weihong Sun, Xueyuan Yuan, Songqing Wu, Pengfei Li, Hui Ding, Lin Ni, Wei Huang, Shuangquan Zou
Format: Article
Language:English
Published: BMC 2018-06-01
Series:Plant Methods
Subjects:
Euscaphis konishii Hayata
Reference gene
qRT-PCR
Transcriptome
Gene expression
Normalization
Online Access:http://link.springer.com/article/10.1186/s13007-018-0311-x

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