Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35
Abstract GYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-74027-7 |
| _version_ | 1818355469047037952 |
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| author | Caroline Dausset Stéphanie Bornes Sylvie Miquel Nathalie Kondjoyan Magaly Angenieux Laurence Nakusi Philippe Veisseire Elina Alaterre Luis G. Bermúdez-Humarán Philippe Langella Erwan Engel Christiane Forestier Adrien Nivoliez |
| author_facet | Caroline Dausset Stéphanie Bornes Sylvie Miquel Nathalie Kondjoyan Magaly Angenieux Laurence Nakusi Philippe Veisseire Elina Alaterre Luis G. Bermúdez-Humarán Philippe Langella Erwan Engel Christiane Forestier Adrien Nivoliez |
| author_sort | Caroline Dausset |
| collection | DOAJ |
| description | Abstract GYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida activity of L. rhamnosus Lcr35, with a complete loss of viability of the yeast after 48 h of coincubation. Sodium thiosulfate (STS), one excipient of the product, was used as a potentiator of the anti-Candida spp. activity of Lactobacilli. This contact-independent phenomenon induced fungal cell disturbances, as observed by electron microscopy observations. Nonverbal sensory experiments showed clear odor dissimilarities between cocultures of L. rhamnosus Lcr35 and C. albicans in the presence and absence of STS, suggesting an impact of odor-active metabolites. A volatolomic approach allowed the identification of six odor-active compounds, including one sulfur compound that was identified as S-methyl thioacetate (MTA). MTA was associated with the antifungal effect of Lcr35, and its functional link was established in vitro. We show for the first time that the LBP GYNOPHILUS, which is a highly active product in the reduction of vulvovaginal candidiasis, requires the presence of a sulfur compound to fully achieve its antifungal effect. |
| first_indexed | 2024-12-13T19:41:48Z |
| format | Article |
| id | doaj.art-64762751c0964b09911a1fa99fa13b36 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T19:41:48Z |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-64762751c0964b09911a1fa99fa13b362022-12-21T23:33:40ZengNature PortfolioScientific Reports2045-23222020-10-0110111210.1038/s41598-020-74027-7Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35Caroline Dausset0Stéphanie Bornes1Sylvie Miquel2Nathalie Kondjoyan3Magaly Angenieux4Laurence Nakusi5Philippe Veisseire6Elina Alaterre7Luis G. Bermúdez-Humarán8Philippe Langella9Erwan Engel10Christiane Forestier11Adrien Nivoliez12Research and Development Department, BIOSEUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRFUniversité Clermont Auvergne, CNRS, Laboratoire Microorganismes : Génome et EnvironnementINRAE, UR370 QuaPA, Microcontaminants, Aroma & Separation Science Group (MASS)INRAE, UR370 QuaPA, Microcontaminants, Aroma & Separation Science Group (MASS)Université Clermont Auvergne, CNRS, Laboratoire Microorganismes : Génome et EnvironnementUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRFResearch and Development Department, BIOSEMicalis Institute, INRAE, AgroParisTech, Université Paris-SaclayMicalis Institute, INRAE, AgroParisTech, Université Paris-SaclayINRAE, UR370 QuaPA, Microcontaminants, Aroma & Separation Science Group (MASS)Université Clermont Auvergne, CNRS, Laboratoire Microorganismes : Génome et EnvironnementResearch and Development Department, BIOSEAbstract GYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida activity of L. rhamnosus Lcr35, with a complete loss of viability of the yeast after 48 h of coincubation. Sodium thiosulfate (STS), one excipient of the product, was used as a potentiator of the anti-Candida spp. activity of Lactobacilli. This contact-independent phenomenon induced fungal cell disturbances, as observed by electron microscopy observations. Nonverbal sensory experiments showed clear odor dissimilarities between cocultures of L. rhamnosus Lcr35 and C. albicans in the presence and absence of STS, suggesting an impact of odor-active metabolites. A volatolomic approach allowed the identification of six odor-active compounds, including one sulfur compound that was identified as S-methyl thioacetate (MTA). MTA was associated with the antifungal effect of Lcr35, and its functional link was established in vitro. We show for the first time that the LBP GYNOPHILUS, which is a highly active product in the reduction of vulvovaginal candidiasis, requires the presence of a sulfur compound to fully achieve its antifungal effect.https://doi.org/10.1038/s41598-020-74027-7 |
| spellingShingle | Caroline Dausset Stéphanie Bornes Sylvie Miquel Nathalie Kondjoyan Magaly Angenieux Laurence Nakusi Philippe Veisseire Elina Alaterre Luis G. Bermúdez-Humarán Philippe Langella Erwan Engel Christiane Forestier Adrien Nivoliez Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 Scientific Reports |
| title | Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 |
| title_full | Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 |
| title_fullStr | Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 |
| title_full_unstemmed | Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 |
| title_short | Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35 |
| title_sort | identification of sulfur components enhancing the anti candida effect of lactobacillus rhamnosus lcr35 |
| url | https://doi.org/10.1038/s41598-020-74027-7 |
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