Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR
Ulcerative colitis is a form of inflammatory bowel disease characterized by chronic inflammation of the colon and rectum. The abnormal lesions in the digestive system caused by ulcerative colitis and intermittent colitis are of major clinical importance. MDM2 is a phospho-protein that functions as a...
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| Format: | Article |
| Language: | English |
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Korea Genome Organization
2020-03-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gi-2020-18-1-e9.pdf |
| _version_ | 1818275424480788480 |
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| author | Mahsa Sadat Hashemi Doulabi Reza Goleyjani Moghaddam Ali Salehzadeh |
| author_facet | Mahsa Sadat Hashemi Doulabi Reza Goleyjani Moghaddam Ali Salehzadeh |
| author_sort | Mahsa Sadat Hashemi Doulabi |
| collection | DOAJ |
| description | Ulcerative colitis is a form of inflammatory bowel disease characterized by chronic inflammation of the colon and rectum. The abnormal lesions in the digestive system caused by ulcerative colitis and intermittent colitis are of major clinical importance. MDM2 is a phospho-protein that functions as a ubiquitin ligase for p53. Recently, a T>G substitution in the promoter of the MDM2 gene (rs309) has been identified. In this case-control study, 174 ulcerative colitis biopsy samples and 82 control samples were collected from colonoscopy centers, hospitals, and clinics in Mazandaran and Gilan Provinces in Iran from October 2014 to May 2015. This MDM2 polymorphism was investigated in DNA samples (extracted from biopsy samples) by amplification-refractory mutation system polymerase chain reaction. The mean age of patients with ulcerative colitis was 46.5 years (range, 28 to 69 years) and that of control individuals was 45.3 years (range, 26 to 71 years). Seventy-eight patients (44.82%) were men and 96 (55.18%) were women. The distribution of the TT, TG, and GG genotypes was 17.93%, 27.59%, and 34.48%, respectively, in the ulcerative colitis patients and 31.70%, 24.40%, and 43.90%, respectively, in the control individuals (odds ratio of GG for ulcerative colitis, 7.142; 95% confidence interval, 2.400 to 9.542; p = 0.001). It was found that a single-nucleotide polymorphism at rs309 in the MDM2 gene was associated with ulcerative colitis. A direct relationship was found between age and ulcerative colitis, while no relationship was found with sex. This finding is of note because the occurrence of intestinal inflammation and subsequent ulcers can precede the development of cancer. |
| first_indexed | 2024-12-12T22:29:32Z |
| format | Article |
| id | doaj.art-64781c691cdd4185ab4dc1aa56c91e40 |
| institution | Directory Open Access Journal |
| issn | 2234-0742 |
| language | English |
| last_indexed | 2024-12-12T22:29:32Z |
| publishDate | 2020-03-01 |
| publisher | Korea Genome Organization |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj.art-64781c691cdd4185ab4dc1aa56c91e402022-12-22T00:09:38ZengKorea Genome OrganizationGenomics & Informatics2234-07422020-03-0118110.5808/GI.2020.18.1.e9601Associations between an gene polymorphism and ulcerative colitis by ARMS-PCRMahsa Sadat Hashemi Doulabi0Reza Goleyjani Moghaddam1Ali Salehzadeh2 Young Researchers and Elite Club, Tonekabon Branch, Islamic Azad University, Tonekabon 46841-61167, Iran Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon 46841-61167, Iran Department of Biology, Rasht Branch, Islamic Azad University, Rasht 41476-54919, IranUlcerative colitis is a form of inflammatory bowel disease characterized by chronic inflammation of the colon and rectum. The abnormal lesions in the digestive system caused by ulcerative colitis and intermittent colitis are of major clinical importance. MDM2 is a phospho-protein that functions as a ubiquitin ligase for p53. Recently, a T>G substitution in the promoter of the MDM2 gene (rs309) has been identified. In this case-control study, 174 ulcerative colitis biopsy samples and 82 control samples were collected from colonoscopy centers, hospitals, and clinics in Mazandaran and Gilan Provinces in Iran from October 2014 to May 2015. This MDM2 polymorphism was investigated in DNA samples (extracted from biopsy samples) by amplification-refractory mutation system polymerase chain reaction. The mean age of patients with ulcerative colitis was 46.5 years (range, 28 to 69 years) and that of control individuals was 45.3 years (range, 26 to 71 years). Seventy-eight patients (44.82%) were men and 96 (55.18%) were women. The distribution of the TT, TG, and GG genotypes was 17.93%, 27.59%, and 34.48%, respectively, in the ulcerative colitis patients and 31.70%, 24.40%, and 43.90%, respectively, in the control individuals (odds ratio of GG for ulcerative colitis, 7.142; 95% confidence interval, 2.400 to 9.542; p = 0.001). It was found that a single-nucleotide polymorphism at rs309 in the MDM2 gene was associated with ulcerative colitis. A direct relationship was found between age and ulcerative colitis, while no relationship was found with sex. This finding is of note because the occurrence of intestinal inflammation and subsequent ulcers can precede the development of cancer.http://genominfo.org/upload/pdf/gi-2020-18-1-e9.pdfinflammatory bowel diseasemalignantpolymorphismulcerative colitis |
| spellingShingle | Mahsa Sadat Hashemi Doulabi Reza Goleyjani Moghaddam Ali Salehzadeh Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR Genomics & Informatics inflammatory bowel disease malignant polymorphism ulcerative colitis |
| title | Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR |
| title_full | Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR |
| title_fullStr | Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR |
| title_full_unstemmed | Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR |
| title_short | Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR |
| title_sort | associations between an gene polymorphism and ulcerative colitis by arms pcr |
| topic | inflammatory bowel disease malignant polymorphism ulcerative colitis |
| url | http://genominfo.org/upload/pdf/gi-2020-18-1-e9.pdf |
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