Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1562 |
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| author | Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou |
| author_facet | Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou |
| author_sort | Vasiliki Zoi |
| collection | DOAJ |
| description | Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation, and the combined effect was compared to the antiproliferative effect of each agent when given individually. Cell viability and proliferation were evaluated with the trypan blue exclusion assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic effects of the combination treatment were analyzed with CompuSyn software. To examine how the co-treatment affected different phases of cell-cycle progression, a cell-cycle analysis via flow cytometry was performed. Treatment with curcumin and radiation significantly reduced cell viability in both U87 and T98 cell lines. The combination treatment arrested both cell lines at the G2/M phase to a higher extent than radiation or curcumin treatment alone. The synergistic effect of curcumin when combined with temozolomide resulted in increased tumor cell death. Our results demonstrate for the first time that low doses of curcumin and irradiation exhibit a strong synergistic anti-proliferative effect on glioblastoma cells in vitro. Therefore, this combination may represent an innovative and promising strategy for the treatment of glioblastoma, and further studies are needed to fully understand the molecular mechanism underlying this effect. |
| first_indexed | 2024-03-10T05:41:16Z |
| format | Article |
| id | doaj.art-647864cbaf384089830a4e5ade7c45c9 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:16Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-647864cbaf384089830a4e5ade7c45c92023-11-22T22:30:04ZengMDPI AGBiomedicines2227-90592021-10-01911156210.3390/biomedicines9111562Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In VitroVasiliki Zoi0Vasiliki Galani1Evrysthenis Vartholomatos2Natalia Zacharopoulou3Eftichia Tsoumeleka4Georgios Gkizas5Georgios Bozios6Pericles Tsekeris7Ieremias Chousidis8Ioannis Leonardos9Andreas G. Tzakos10Athanasios P. Kyritsis11George A. Alexiou12Neurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceDepartment of Anatomy Histology-Embryology, School of Medicine, University of Ioannina, 45110 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceDepartment of Medical Physics, University of Ioannina, 45110 Ioannina, GreeceDepartment of Radiation Oncology, University of Ioannina, 45110 Ioannina, GreeceZoology Laboratory, Department of Biological Application and Technology, University of Ioannina, 45110 Ioannina, GreeceZoology Laboratory, Department of Biological Application and Technology, University of Ioannina, 45110 Ioannina, GreeceDepartment of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceNeurosurgical Institute, University of Ioannina, 45500 Ioannina, GreeceCurcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation, and the combined effect was compared to the antiproliferative effect of each agent when given individually. Cell viability and proliferation were evaluated with the trypan blue exclusion assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic effects of the combination treatment were analyzed with CompuSyn software. To examine how the co-treatment affected different phases of cell-cycle progression, a cell-cycle analysis via flow cytometry was performed. Treatment with curcumin and radiation significantly reduced cell viability in both U87 and T98 cell lines. The combination treatment arrested both cell lines at the G2/M phase to a higher extent than radiation or curcumin treatment alone. The synergistic effect of curcumin when combined with temozolomide resulted in increased tumor cell death. Our results demonstrate for the first time that low doses of curcumin and irradiation exhibit a strong synergistic anti-proliferative effect on glioblastoma cells in vitro. Therefore, this combination may represent an innovative and promising strategy for the treatment of glioblastoma, and further studies are needed to fully understand the molecular mechanism underlying this effect.https://www.mdpi.com/2227-9059/9/11/1562curcuminglioblastoma cellsradiation |
| spellingShingle | Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro Biomedicines curcumin glioblastoma cells radiation |
| title | Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro |
| title_full | Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro |
| title_fullStr | Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro |
| title_full_unstemmed | Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro |
| title_short | Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro |
| title_sort | curcumin and radiotherapy exert synergistic anti glioma effect in vitro |
| topic | curcumin glioblastoma cells radiation |
| url | https://www.mdpi.com/2227-9059/9/11/1562 |
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