Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Pharmaceutics |
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| Online Access: | http://www.mdpi.com/1999-4923/11/1/25 |
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| author | Olga A. Krasheninina Evgeny K. Apartsin Elena Fuentes Aleksandra Szulc Maksim Ionov Alya G. Venyaminova Dzmitry Shcharbin F. Javier de la Mata Maria Bryszewska Rafael Gόmez |
| author_facet | Olga A. Krasheninina Evgeny K. Apartsin Elena Fuentes Aleksandra Szulc Maksim Ionov Alya G. Venyaminova Dzmitry Shcharbin F. Javier de la Mata Maria Bryszewska Rafael Gόmez |
| author_sort | Olga A. Krasheninina |
| collection | DOAJ |
| description | This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells. |
| first_indexed | 2024-04-11T22:10:01Z |
| format | Article |
| id | doaj.art-6479b0ad8ff34feebcaed11a609cd614 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T22:10:01Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-6479b0ad8ff34feebcaed11a609cd6142022-12-22T04:00:36ZengMDPI AGPharmaceutics1999-49232019-01-011112510.3390/pharmaceutics11010025pharmaceutics11010025Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer CellsOlga A. Krasheninina0Evgeny K. Apartsin1Elena Fuentes2Aleksandra Szulc3Maksim Ionov4Alya G. Venyaminova5Dzmitry Shcharbin6F. Javier de la Mata7Maria Bryszewska8Rafael Gόmez9Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Biophysics and Cell Engineering of NASB, 220072 Minsk, BelarusDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainDepartment of General Biophysics, University of Lodz, 90-236 Lodz, PolandDepartamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, SpainThis paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.http://www.mdpi.com/1999-4923/11/1/25carbosilanedendrimersanticancer siRNAnucleic acidsdendriplexescomplexationtransfectioncytotoxicitybiophysical assays |
| spellingShingle | Olga A. Krasheninina Evgeny K. Apartsin Elena Fuentes Aleksandra Szulc Maksim Ionov Alya G. Venyaminova Dzmitry Shcharbin F. Javier de la Mata Maria Bryszewska Rafael Gόmez Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells Pharmaceutics carbosilane dendrimers anticancer siRNA nucleic acids dendriplexes complexation transfection cytotoxicity biophysical assays |
| title | Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells |
| title_full | Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells |
| title_fullStr | Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells |
| title_full_unstemmed | Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells |
| title_short | Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells |
| title_sort | complexes of pro apoptotic sirnas and carbosilane dendrimers formation and effect on cancer cells |
| topic | carbosilane dendrimers anticancer siRNA nucleic acids dendriplexes complexation transfection cytotoxicity biophysical assays |
| url | http://www.mdpi.com/1999-4923/11/1/25 |
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