The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses
Abstract Ozonated water has become an innovative, environmentally friendly tool for controlling the development of fungal diseases in the vineyard or during grape postharvest conservation. However, little information is currently available on the effects of ozonated water sprayings on the grapevine...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87542-y |
| _version_ | 1818858329277988864 |
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| author | Ana Campayo Stefania Savoi Charles Romieu Alberto José López-Jiménez Kortes Serrano de la Hoz M. Rosario Salinas Laurent Torregrosa Gonzalo L. Alonso |
| author_facet | Ana Campayo Stefania Savoi Charles Romieu Alberto José López-Jiménez Kortes Serrano de la Hoz M. Rosario Salinas Laurent Torregrosa Gonzalo L. Alonso |
| author_sort | Ana Campayo |
| collection | DOAJ |
| description | Abstract Ozonated water has become an innovative, environmentally friendly tool for controlling the development of fungal diseases in the vineyard or during grape postharvest conservation. However, little information is currently available on the effects of ozonated water sprayings on the grapevine physiology and metabolism. Using the microvine model, we studied the transcriptomic response of leaf and fruit organs to this treatment. The response to ozone was observed to be organ and developmental stage-dependent, with a decrease of the number of DEGs (differentially expressed genes) in the fruit from the onset of ripening to later stages. The most highly up-regulated gene families were heat-shock proteins and chaperones. Other up-regulated genes were involved in oxidative stress homeostasis such as those of the ascorbate–glutathione cycle and glutathione S-transferases. In contrast, genes related to cell wall development and secondary metabolites (carotenoids, terpenoids, phenylpropanoids / flavonoids) were generally down-regulated after ozone treatment, mainly in the early stage of fruit ripening. This down-regulation may indicate a possible carbon competition favouring the re-establishment and maintenance of the redox homeostasis rather than the synthesis of secondary metabolites at the beginning of ripening, the most ozone responsive developmental stage. |
| first_indexed | 2024-12-19T08:54:33Z |
| format | Article |
| id | doaj.art-647fdb8064664a7da2cf47253f689ed2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:54:33Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-647fdb8064664a7da2cf47253f689ed22022-12-21T20:28:39ZengNature PortfolioScientific Reports2045-23222021-04-0111111410.1038/s41598-021-87542-yThe application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responsesAna Campayo0Stefania Savoi1Charles Romieu2Alberto José López-Jiménez3Kortes Serrano de la Hoz4M. Rosario Salinas5Laurent Torregrosa6Gonzalo L. Alonso7Cátedra de Química Agrícola, E.T.S.I. Agrónomos y de Montes, Universidad de Castilla-La ManchaAGAP, CIRAD, INRAe, Institut Agro-Montpellier SupAgro, Montpellier UniversityAGAP, CIRAD, INRAe, Institut Agro-Montpellier SupAgro, Montpellier UniversityDepartamento de Ciencia y Tecnología Agroforestal y Genética, Universidad de Castilla-La ManchaBetterRID (Better Research, Innovation and Development, S.L.)Cátedra de Química Agrícola, E.T.S.I. Agrónomos y de Montes, Universidad de Castilla-La ManchaAGAP, CIRAD, INRAe, Institut Agro-Montpellier SupAgro, Montpellier UniversityCátedra de Química Agrícola, E.T.S.I. Agrónomos y de Montes, Universidad de Castilla-La ManchaAbstract Ozonated water has become an innovative, environmentally friendly tool for controlling the development of fungal diseases in the vineyard or during grape postharvest conservation. However, little information is currently available on the effects of ozonated water sprayings on the grapevine physiology and metabolism. Using the microvine model, we studied the transcriptomic response of leaf and fruit organs to this treatment. The response to ozone was observed to be organ and developmental stage-dependent, with a decrease of the number of DEGs (differentially expressed genes) in the fruit from the onset of ripening to later stages. The most highly up-regulated gene families were heat-shock proteins and chaperones. Other up-regulated genes were involved in oxidative stress homeostasis such as those of the ascorbate–glutathione cycle and glutathione S-transferases. In contrast, genes related to cell wall development and secondary metabolites (carotenoids, terpenoids, phenylpropanoids / flavonoids) were generally down-regulated after ozone treatment, mainly in the early stage of fruit ripening. This down-regulation may indicate a possible carbon competition favouring the re-establishment and maintenance of the redox homeostasis rather than the synthesis of secondary metabolites at the beginning of ripening, the most ozone responsive developmental stage.https://doi.org/10.1038/s41598-021-87542-y |
| spellingShingle | Ana Campayo Stefania Savoi Charles Romieu Alberto José López-Jiménez Kortes Serrano de la Hoz M. Rosario Salinas Laurent Torregrosa Gonzalo L. Alonso The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses Scientific Reports |
| title | The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses |
| title_full | The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses |
| title_fullStr | The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses |
| title_full_unstemmed | The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses |
| title_short | The application of ozonated water rearranges the Vitis vinifera L. leaf and berry transcriptomes eliciting defence and antioxidant responses |
| title_sort | application of ozonated water rearranges the vitis vinifera l leaf and berry transcriptomes eliciting defence and antioxidant responses |
| url | https://doi.org/10.1038/s41598-021-87542-y |
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