Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes
One important pharmacological function of hyaluronic acid (HA) in chondrocytes is reduction of cellular superoxide generation and accumulation. Here we demonstrated a relationship between HA supplementation and accumulation of Nuclear factor‐erythroid‐2‐related factor 2 (Nrf2), which is a master tra...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.fob.2015.05.007 |
| _version_ | 1828274636822937600 |
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| author | Yuta Onodera Takeshi Teramura Toshiyuki Takehara Kanji Fukuda |
| author_facet | Yuta Onodera Takeshi Teramura Toshiyuki Takehara Kanji Fukuda |
| author_sort | Yuta Onodera |
| collection | DOAJ |
| description | One important pharmacological function of hyaluronic acid (HA) in chondrocytes is reduction of cellular superoxide generation and accumulation. Here we demonstrated a relationship between HA supplementation and accumulation of Nuclear factor‐erythroid‐2‐related factor 2 (Nrf2), which is a master transcription factor in cellular redox reactions, in cultured chondrocytes derived from bovine joint cartilage. In HA‐treated chondrocytes, expression of Nrf2 and its downstream genes was upregulated. In HA‐treated chondrocytes, Akt was phosphorylated, and inhibition of Akt activity or suppression of HA receptors CD44 and/or RHAMM with siRNAs prevented HA‐mediated Nrf2 accumulation. Furthermore, Nrf2 siRNA inhibited the HA effect on antioxidant enzymes. These results show that HA might contribute to ROS reduction through Nrf2 regulation by activating Akt. Our study suggests a new mechanism for extracellular matrix (ECM)‐mediated redox systems in chondrocytes. |
| first_indexed | 2024-04-13T06:37:30Z |
| format | Article |
| id | doaj.art-6481c50207494948b790650f2534062e |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-13T06:37:30Z |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-6481c50207494948b790650f2534062e2022-12-22T02:57:50ZengWileyFEBS Open Bio2211-54632015-01-015147648410.1016/j.fob.2015.05.007Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytesYuta Onodera0Takeshi Teramura1Toshiyuki Takehara2Kanji Fukuda3Institute of Advanced Clinical Medicine, Kindai University Faculty of Medicine, Osakasayama, Osaka, JapanInstitute of Advanced Clinical Medicine, Kindai University Faculty of Medicine, Osakasayama, Osaka, JapanInstitute of Advanced Clinical Medicine, Kindai University Faculty of Medicine, Osakasayama, Osaka, JapanInstitute of Advanced Clinical Medicine, Kindai University Faculty of Medicine, Osakasayama, Osaka, JapanOne important pharmacological function of hyaluronic acid (HA) in chondrocytes is reduction of cellular superoxide generation and accumulation. Here we demonstrated a relationship between HA supplementation and accumulation of Nuclear factor‐erythroid‐2‐related factor 2 (Nrf2), which is a master transcription factor in cellular redox reactions, in cultured chondrocytes derived from bovine joint cartilage. In HA‐treated chondrocytes, expression of Nrf2 and its downstream genes was upregulated. In HA‐treated chondrocytes, Akt was phosphorylated, and inhibition of Akt activity or suppression of HA receptors CD44 and/or RHAMM with siRNAs prevented HA‐mediated Nrf2 accumulation. Furthermore, Nrf2 siRNA inhibited the HA effect on antioxidant enzymes. These results show that HA might contribute to ROS reduction through Nrf2 regulation by activating Akt. Our study suggests a new mechanism for extracellular matrix (ECM)‐mediated redox systems in chondrocytes.https://doi.org/10.1016/j.fob.2015.05.007Hyaluronic acidReactive oxygen species (ROS)Nuclear factor-erythroid-2-related factor 2 (Nrf2)Chondrocytes |
| spellingShingle | Yuta Onodera Takeshi Teramura Toshiyuki Takehara Kanji Fukuda Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes FEBS Open Bio Hyaluronic acid Reactive oxygen species (ROS) Nuclear factor-erythroid-2-related factor 2 (Nrf2) Chondrocytes |
| title | Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes |
| title_full | Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes |
| title_fullStr | Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes |
| title_full_unstemmed | Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes |
| title_short | Hyaluronic acid regulates a key redox control factor Nrf2 via phosphorylation of Akt in bovine articular chondrocytes |
| title_sort | hyaluronic acid regulates a key redox control factor nrf2 via phosphorylation of akt in bovine articular chondrocytes |
| topic | Hyaluronic acid Reactive oxygen species (ROS) Nuclear factor-erythroid-2-related factor 2 (Nrf2) Chondrocytes |
| url | https://doi.org/10.1016/j.fob.2015.05.007 |
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