In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions
An efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021002061 |
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| author | Vineet Soni Kiran Keswani Upma Bhatt Deepak Kumar Hanwant Singh |
| author_facet | Vineet Soni Kiran Keswani Upma Bhatt Deepak Kumar Hanwant Singh |
| author_sort | Vineet Soni |
| collection | DOAJ |
| description | An efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll a fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 ± 0.07) and shoot length (4.7 ± 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l−1 BAP and 1.0 mg l−1 TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l−1 IAA. In vitro plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when in vitro plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed in vitro protocol will be useful for genetic improvement and multiplication of D. unguis-cati. The results of this study also show that photosynthetic screening of in vitro developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions. |
| first_indexed | 2024-12-17T08:35:56Z |
| format | Article |
| id | doaj.art-648296f31356481aa1c3e2ba3a3343b4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-17T08:35:56Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-648296f31356481aa1c3e2ba3a3343b42022-12-21T21:56:28ZengElsevierHeliyon2405-84402021-02-0172e06101In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditionsVineet Soni0Kiran Keswani1Upma Bhatt2Deepak Kumar3Hanwant Singh4Plant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India; Corresponding author.Plant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India; Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune 411045, Maharashtra, IndiaPlant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, IndiaPlant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, IndiaPlant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, IndiaAn efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll a fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 ± 0.07) and shoot length (4.7 ± 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l−1 BAP and 1.0 mg l−1 TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l−1 IAA. In vitro plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when in vitro plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed in vitro protocol will be useful for genetic improvement and multiplication of D. unguis-cati. The results of this study also show that photosynthetic screening of in vitro developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions.http://www.sciencedirect.com/science/article/pii/S2405844021002061In vitro propagationChlorophyll a fluorescenceJIP testFv/FmPerformance index |
| spellingShingle | Vineet Soni Kiran Keswani Upma Bhatt Deepak Kumar Hanwant Singh In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions Heliyon In vitro propagation Chlorophyll a fluorescence JIP test Fv/Fm Performance index |
| title | In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions |
| title_full | In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions |
| title_fullStr | In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions |
| title_full_unstemmed | In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions |
| title_short | In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions |
| title_sort | in vitro propagation and analysis of mixotrophic potential to improve survival rate of dolichandra unguis cati under ex vitro conditions |
| topic | In vitro propagation Chlorophyll a fluorescence JIP test Fv/Fm Performance index |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021002061 |
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