Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF)
Both essential and non-essential elements have been associated with female reproductive function in epidemiologic investigations, including among IVF populations. To date, most investigators have used blood or urine to assess biomarkers of exposure, with few employing ovarian follicular fluid (FF)....
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651320315700 |
| _version_ | 1819276681426239488 |
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| author | Celeste D. Butts Michael S. Bloom Alexandra McGough Nikolaus Lenhart Rebecca Wong Evelyn Mok-Lin Patrick J. Parsons Aubrey L. Galusha Recai M. Yucel Beth J. Feingold Richard W. Browne Victor Y. Fujimoto |
| author_facet | Celeste D. Butts Michael S. Bloom Alexandra McGough Nikolaus Lenhart Rebecca Wong Evelyn Mok-Lin Patrick J. Parsons Aubrey L. Galusha Recai M. Yucel Beth J. Feingold Richard W. Browne Victor Y. Fujimoto |
| author_sort | Celeste D. Butts |
| collection | DOAJ |
| description | Both essential and non-essential elements have been associated with female reproductive function in epidemiologic investigations, including among IVF populations. To date, most investigators have used blood or urine to assess biomarkers of exposure, with few employing ovarian follicular fluid (FF). FF may offer a more direct “snapshot” of the oocyte microenvironment than blood or urine, however previous studies report follicle-to-follicle variability in FF constituents that may contribute to exposure misclassification. Our objectives were to investigate sources of trace element variability, to estimate FF biomarker reliability among women undergoing IVF (n = 34), and to determine the minimum number of follicles required to estimate subject-specific mean concentrations. We measured As, Hg, Cd, Pb, Cu, Mn, Se, and Zn in FF samples using inductively coupled plasma tandem mass spectrometry. Inter-subject (between-women) variability contributed most of the variability in FF element concentrations, with ovarian, follicular, and analytical as smaller sources of variability. The proportion of variability attributable to sources between-follicles differed by age, body mass index (BMI), race, and cigarette smoking for Cu, Se, and Zn, by BMI and cigarette smoking for As, by primary infertility diagnosis for Hg, Cu, Se, and Zn, and by ovarian stimulation protocol for Mn and Se. Four to five individual follicles were sufficient to estimate subject-specific mean Cu, Se, and Zn concentrations, while >14 were necessary for As, Hg, Cd, Pb, and Mn. Overall, our results suggest that FF is a suitable source of biomarkers of As and Hg exposure in ovarian follicles. Although limited in size, our study offers the most comprehensive exploration of biological variation in FF trace elements to date and may provide guidance for future studies of ovarian trace element exposures. |
| first_indexed | 2024-12-23T23:44:05Z |
| format | Article |
| id | doaj.art-6484ca26f3ec49c79c46e94d820138ae |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-23T23:44:05Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-6484ca26f3ec49c79c46e94d820138ae2022-12-21T17:25:34ZengElsevierEcotoxicology and Environmental Safety0147-65132021-02-01209111733Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF)Celeste D. Butts0Michael S. Bloom1Alexandra McGough2Nikolaus Lenhart3Rebecca Wong4Evelyn Mok-Lin5Patrick J. Parsons6Aubrey L. Galusha7Recai M. Yucel8Beth J. Feingold9Richard W. Browne10Victor Y. Fujimoto11Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY 12144, United StatesDepartment of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY 12144, United States; Department of Epidemiology & Biostatistics, University at Albany, State University of New York, Rensselaer, NY 12144, United States; Department of Global and Community Health, George Mason University, Fairfax, VA 22030 United States; Correspondence to: Department of Global and Community Health, George Mason University, 4400 University Drive, MS 5B7, Fairfax, VA 22030, United States.Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94158, United StatesDepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94158, United StatesDepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94158, United StatesDepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94158, United StatesDepartment of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY 12144, United States; Laboratory of Inorganic & Nuclear Chemistry, Wadsworth Center, New York State Department of Health, Albany, NY 12201, United StatesDepartment of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY 12144, United States; Laboratory of Inorganic & Nuclear Chemistry, Wadsworth Center, New York State Department of Health, Albany, NY 12201, United StatesDepartment of Epidemiology and Biostatistics, Temple University, Philadelphia, PA 19122, United StatesDepartment of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY 12144, United StatesDepartment of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260, United StatesDepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94158, United StatesBoth essential and non-essential elements have been associated with female reproductive function in epidemiologic investigations, including among IVF populations. To date, most investigators have used blood or urine to assess biomarkers of exposure, with few employing ovarian follicular fluid (FF). FF may offer a more direct “snapshot” of the oocyte microenvironment than blood or urine, however previous studies report follicle-to-follicle variability in FF constituents that may contribute to exposure misclassification. Our objectives were to investigate sources of trace element variability, to estimate FF biomarker reliability among women undergoing IVF (n = 34), and to determine the minimum number of follicles required to estimate subject-specific mean concentrations. We measured As, Hg, Cd, Pb, Cu, Mn, Se, and Zn in FF samples using inductively coupled plasma tandem mass spectrometry. Inter-subject (between-women) variability contributed most of the variability in FF element concentrations, with ovarian, follicular, and analytical as smaller sources of variability. The proportion of variability attributable to sources between-follicles differed by age, body mass index (BMI), race, and cigarette smoking for Cu, Se, and Zn, by BMI and cigarette smoking for As, by primary infertility diagnosis for Hg, Cu, Se, and Zn, and by ovarian stimulation protocol for Mn and Se. Four to five individual follicles were sufficient to estimate subject-specific mean Cu, Se, and Zn concentrations, while >14 were necessary for As, Hg, Cd, Pb, and Mn. Overall, our results suggest that FF is a suitable source of biomarkers of As and Hg exposure in ovarian follicles. Although limited in size, our study offers the most comprehensive exploration of biological variation in FF trace elements to date and may provide guidance for future studies of ovarian trace element exposures.http://www.sciencedirect.com/science/article/pii/S0147651320315700Biologic variabilityBiomarkersFollicular fluidIn vitro fertilizationReliabilityTrace elements |
| spellingShingle | Celeste D. Butts Michael S. Bloom Alexandra McGough Nikolaus Lenhart Rebecca Wong Evelyn Mok-Lin Patrick J. Parsons Aubrey L. Galusha Recai M. Yucel Beth J. Feingold Richard W. Browne Victor Y. Fujimoto Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) Ecotoxicology and Environmental Safety Biologic variability Biomarkers Follicular fluid In vitro fertilization Reliability Trace elements |
| title | Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) |
| title_full | Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) |
| title_fullStr | Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) |
| title_full_unstemmed | Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) |
| title_short | Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF) |
| title_sort | variability of essential and non essential trace elements in the follicular fluid of women undergoing in vitro fertilization ivf |
| topic | Biologic variability Biomarkers Follicular fluid In vitro fertilization Reliability Trace elements |
| url | http://www.sciencedirect.com/science/article/pii/S0147651320315700 |
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