Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping
Drought seriously curtails growth, physiology and productivity in rapeseed (Brassica napus). Although drought tolerance is a complex trait, efficient phenotyping and genotyping has led to the identification of novel marker-trait associations underlying drought tolerance. A diverse panel of 228 Brass...
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| Language: | English |
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Elsevier
2020-07-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123220301016 |
| _version_ | 1831727286499409920 |
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| author | Hira Khanzada Ghulam Mustafa Wassan Haohua He Annaliese S. Mason Ayaz Ali Keerio Saba Khanzada Muhammad Faheem Abdul Malik Solangi Qinghong Zhou Donghui Fu Yingjin Huang Adnan Rasheed |
| author_facet | Hira Khanzada Ghulam Mustafa Wassan Haohua He Annaliese S. Mason Ayaz Ali Keerio Saba Khanzada Muhammad Faheem Abdul Malik Solangi Qinghong Zhou Donghui Fu Yingjin Huang Adnan Rasheed |
| author_sort | Hira Khanzada |
| collection | DOAJ |
| description | Drought seriously curtails growth, physiology and productivity in rapeseed (Brassica napus). Although drought tolerance is a complex trait, efficient phenotyping and genotyping has led to the identification of novel marker-trait associations underlying drought tolerance. A diverse panel of 228 Brassica accessions was phenotyped under normal (without stress) and water-stress conditions, simulated by polyethylene glycol (PEG-6000) (15% PEG stress) at the seedling stage; stress tolerance index (STI) and stress susceptibility index (SSI) values were acquired. Genome-wide association studies (GWAS) using 201 817 high quality SNPs identified 314 marker-trait associations strongly linked with drought indices and distributed across all nineteen chromosomes in both the A and C genomes. None of these quantitative trait loci (QTL) had been previously identified by other studies. We identified 85 genes underlying these QTL (most within 100 kb of associated SNPs) which were orthologous to Arabidopsis genes known to be associated with drought tolerance. Our study provides a novel resource for breeding drought-tolerant Brassica crops. |
| first_indexed | 2024-12-21T06:26:36Z |
| format | Article |
| id | doaj.art-64850a2984854f6fb12346af36799234 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-12-21T06:26:36Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-64850a2984854f6fb12346af367992342022-12-21T19:13:06ZengElsevierJournal of Advanced Research2090-12322020-07-0124447461Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mappingHira Khanzada0Ghulam Mustafa Wassan1Haohua He2Annaliese S. Mason3Ayaz Ali Keerio4Saba Khanzada5Muhammad Faheem6Abdul Malik Solangi7Qinghong Zhou8Donghui Fu9Yingjin Huang10Adnan Rasheed11Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaDepartment of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, GermanyDepartment of Plant Breeding and Genetics, Faculty of Crop Production, Sindh Agriculture University Tando Jam, Sindh, PakistanKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Land Resources and Environment, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors at: College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors at: College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding authors at: College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, ChinaDrought seriously curtails growth, physiology and productivity in rapeseed (Brassica napus). Although drought tolerance is a complex trait, efficient phenotyping and genotyping has led to the identification of novel marker-trait associations underlying drought tolerance. A diverse panel of 228 Brassica accessions was phenotyped under normal (without stress) and water-stress conditions, simulated by polyethylene glycol (PEG-6000) (15% PEG stress) at the seedling stage; stress tolerance index (STI) and stress susceptibility index (SSI) values were acquired. Genome-wide association studies (GWAS) using 201 817 high quality SNPs identified 314 marker-trait associations strongly linked with drought indices and distributed across all nineteen chromosomes in both the A and C genomes. None of these quantitative trait loci (QTL) had been previously identified by other studies. We identified 85 genes underlying these QTL (most within 100 kb of associated SNPs) which were orthologous to Arabidopsis genes known to be associated with drought tolerance. Our study provides a novel resource for breeding drought-tolerant Brassica crops.http://www.sciencedirect.com/science/article/pii/S2090123220301016Brassica napusDrought toleranceGenome-wide association studies (GWAS)Specific length amplified fragments (SLAFs)Candidate genes |
| spellingShingle | Hira Khanzada Ghulam Mustafa Wassan Haohua He Annaliese S. Mason Ayaz Ali Keerio Saba Khanzada Muhammad Faheem Abdul Malik Solangi Qinghong Zhou Donghui Fu Yingjin Huang Adnan Rasheed Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping Journal of Advanced Research Brassica napus Drought tolerance Genome-wide association studies (GWAS) Specific length amplified fragments (SLAFs) Candidate genes |
| title | Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping |
| title_full | Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping |
| title_fullStr | Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping |
| title_full_unstemmed | Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping |
| title_short | Differentially evolved drought stress indices determine the genetic variation of Brassica napus at seedling traits by genome-wide association mapping |
| title_sort | differentially evolved drought stress indices determine the genetic variation of brassica napus at seedling traits by genome wide association mapping |
| topic | Brassica napus Drought tolerance Genome-wide association studies (GWAS) Specific length amplified fragments (SLAFs) Candidate genes |
| url | http://www.sciencedirect.com/science/article/pii/S2090123220301016 |
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