Development and Notch signaling requirements of the zebrafish choroid plexus.
The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of e...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-09-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2528000?pdf=render |
| _version_ | 1819122738251431936 |
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| author | Brent R Bill Darius Balciunas Joshua A McCarra Eric D Young Toua Xiong Ashley M Spahn Marta Garcia-Lecea Vladimir Korzh Stephen C Ekker Lisa A Schimmenti |
| author_facet | Brent R Bill Darius Balciunas Joshua A McCarra Eric D Young Toua Xiong Ashley M Spahn Marta Garcia-Lecea Vladimir Korzh Stephen C Ekker Lisa A Schimmenti |
| author_sort | Brent R Bill |
| collection | DOAJ |
| description | The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms.Here we show that the transposon-mediated enhancer trap zebrafish line Et(Mn16) expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the definitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA, and deltaD.This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculature integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain. |
| first_indexed | 2024-12-22T06:57:13Z |
| format | Article |
| id | doaj.art-6485632654c84514a005e0a52e44ba48 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T06:57:13Z |
| publishDate | 2008-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6485632654c84514a005e0a52e44ba482022-12-21T18:34:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-09-0139e311410.1371/journal.pone.0003114Development and Notch signaling requirements of the zebrafish choroid plexus.Brent R BillDarius BalciunasJoshua A McCarraEric D YoungToua XiongAshley M SpahnMarta Garcia-LeceaVladimir KorzhStephen C EkkerLisa A SchimmentiThe choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms.Here we show that the transposon-mediated enhancer trap zebrafish line Et(Mn16) expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the definitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA, and deltaD.This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculature integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain.http://europepmc.org/articles/PMC2528000?pdf=render |
| spellingShingle | Brent R Bill Darius Balciunas Joshua A McCarra Eric D Young Toua Xiong Ashley M Spahn Marta Garcia-Lecea Vladimir Korzh Stephen C Ekker Lisa A Schimmenti Development and Notch signaling requirements of the zebrafish choroid plexus. PLoS ONE |
| title | Development and Notch signaling requirements of the zebrafish choroid plexus. |
| title_full | Development and Notch signaling requirements of the zebrafish choroid plexus. |
| title_fullStr | Development and Notch signaling requirements of the zebrafish choroid plexus. |
| title_full_unstemmed | Development and Notch signaling requirements of the zebrafish choroid plexus. |
| title_short | Development and Notch signaling requirements of the zebrafish choroid plexus. |
| title_sort | development and notch signaling requirements of the zebrafish choroid plexus |
| url | http://europepmc.org/articles/PMC2528000?pdf=render |
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