Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma
Aim: The aim of this study was to observe the possible change of microRNAs (miRNAs) in serum extracellular vesicles (EVs) from hepatocellular carcinoma (HCC) patients.Methods: The serum EVs were purified from 17 healthy donors, 16 chronic hepatitis B (CHB) patients and 24 HCC patients. The sequenced...
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.00930/full |
| _version_ | 1818059663137046528 |
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| author | Chunwen Pu Chunwen Pu Hui Huang Zhidong Wang Wei Zou Yuecai Lv Zhiyuan Zhou Qiqi Zhang Liang Qiao Fei Wu Shujuan Shao |
| author_facet | Chunwen Pu Chunwen Pu Hui Huang Zhidong Wang Wei Zou Yuecai Lv Zhiyuan Zhou Qiqi Zhang Liang Qiao Fei Wu Shujuan Shao |
| author_sort | Chunwen Pu |
| collection | DOAJ |
| description | Aim: The aim of this study was to observe the possible change of microRNAs (miRNAs) in serum extracellular vesicles (EVs) from hepatocellular carcinoma (HCC) patients.Methods: The serum EVs were purified from 17 healthy donors, 16 chronic hepatitis B (CHB) patients and 24 HCC patients. The sequenced microRNAs in the purified EVs were analyzed to obtain highly differentially expressed genes (DEGs). Finally, the expression pattern of DEGs was validated using qRT-PCR.Results: We found that the expression of hsa-miR-21-5p and hsa-miR-144-3p were significantly higher in EVs and liver cancer tissues compared with serum and the distal liver tissues in HCC patients. The ratio of hsa-miR-144-3p/hsa-miR-21-5p was significantly decreased in the patients with CHB but significantly increased in patients with HCC developed from CHB (P < 0.05). Hsa-144-3p/hsa-miR-21-5p exhibited greater performance than alpha-fetoprotein (AUC 0.780, 95% CI 0.601–0.960, versus AUC 0.626, 95% CI 0.410–0.843) in ROC curve analysis.Conclusion: Extracellular vesicle-associated hsa-miR-21-5p and hsa-miR-144-3p are markedly elevated in serum of patients with HCC. The potential role of these microRNAs in the pathogenesis of HCC is worth of further study. |
| first_indexed | 2024-12-10T13:20:06Z |
| format | Article |
| id | doaj.art-64882a7daba74b1cb0e924a59c872eaf |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T13:20:06Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-64882a7daba74b1cb0e924a59c872eaf2022-12-22T01:47:22ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-07-01910.3389/fphys.2018.00930353127Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular CarcinomaChunwen Pu0Chunwen Pu1Hui Huang2Zhidong Wang3Wei Zou4Yuecai Lv5Zhiyuan Zhou6Qiqi Zhang7Liang Qiao8Fei Wu9Shujuan Shao10Department of Biobank, The Affiliated Sixth People’s Hospital of Dalian Medical University, Dalian, ChinaThe Affiliated Zhongshan Hospital of Dalian University, Dalian, ChinaDepartment of Biobank, The Affiliated Sixth People’s Hospital of Dalian Medical University, Dalian, ChinaDepartment of Biobank, The Affiliated Sixth People’s Hospital of Dalian Medical University, Dalian, ChinaCollege of Life Science, Liaoning Normal University, Dalian, ChinaKey Laboratory of Proteomics, Dalian Medical University, Dalian, ChinaCollege of Life Science, Liaoning Normal University, Dalian, ChinaDepartment of Biobank, The Affiliated Sixth People’s Hospital of Dalian Medical University, Dalian, ChinaThe University of Sydney at Westmead Hospital, Westmead, NSW, AustraliaThe Affiliated Zhongshan Hospital of Dalian University, Dalian, ChinaKey Laboratory of Proteomics, Dalian Medical University, Dalian, ChinaAim: The aim of this study was to observe the possible change of microRNAs (miRNAs) in serum extracellular vesicles (EVs) from hepatocellular carcinoma (HCC) patients.Methods: The serum EVs were purified from 17 healthy donors, 16 chronic hepatitis B (CHB) patients and 24 HCC patients. The sequenced microRNAs in the purified EVs were analyzed to obtain highly differentially expressed genes (DEGs). Finally, the expression pattern of DEGs was validated using qRT-PCR.Results: We found that the expression of hsa-miR-21-5p and hsa-miR-144-3p were significantly higher in EVs and liver cancer tissues compared with serum and the distal liver tissues in HCC patients. The ratio of hsa-miR-144-3p/hsa-miR-21-5p was significantly decreased in the patients with CHB but significantly increased in patients with HCC developed from CHB (P < 0.05). Hsa-144-3p/hsa-miR-21-5p exhibited greater performance than alpha-fetoprotein (AUC 0.780, 95% CI 0.601–0.960, versus AUC 0.626, 95% CI 0.410–0.843) in ROC curve analysis.Conclusion: Extracellular vesicle-associated hsa-miR-21-5p and hsa-miR-144-3p are markedly elevated in serum of patients with HCC. The potential role of these microRNAs in the pathogenesis of HCC is worth of further study.https://www.frontiersin.org/article/10.3389/fphys.2018.00930/fullmicroRNAextracellular vesiclesequencing analysisqRT-PCRhepatocellular carcinoma |
| spellingShingle | Chunwen Pu Chunwen Pu Hui Huang Zhidong Wang Wei Zou Yuecai Lv Zhiyuan Zhou Qiqi Zhang Liang Qiao Fei Wu Shujuan Shao Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma Frontiers in Physiology microRNA extracellular vesicle sequencing analysis qRT-PCR hepatocellular carcinoma |
| title | Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma |
| title_full | Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma |
| title_fullStr | Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma |
| title_full_unstemmed | Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma |
| title_short | Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma |
| title_sort | extracellular vesicle associated mir 21 and mir 144 are markedly elevated in serum of patients with hepatocellular carcinoma |
| topic | microRNA extracellular vesicle sequencing analysis qRT-PCR hepatocellular carcinoma |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.00930/full |
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