Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor
The kisspeptin receptor, crucial for hypothalamic control of puberty and reproduction, is also present in the pituitary gland. Its role in the pituitary gland is not defined. Kisspeptin signaling via the Kiss1r could potentially regulate reproductive function at the level of pituitary gonadotrope. U...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.868593/full |
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| author | Yaping Ma Yaping Ma Olubusayo Awe Sally Radovick Xiaofeng Yang Sara Divall Andrew Wolfe Sheng Wu Sheng Wu Sheng Wu |
| author_facet | Yaping Ma Yaping Ma Olubusayo Awe Sally Radovick Xiaofeng Yang Sara Divall Andrew Wolfe Sheng Wu Sheng Wu Sheng Wu |
| author_sort | Yaping Ma |
| collection | DOAJ |
| description | The kisspeptin receptor, crucial for hypothalamic control of puberty and reproduction, is also present in the pituitary gland. Its role in the pituitary gland is not defined. Kisspeptin signaling via the Kiss1r could potentially regulate reproductive function at the level of pituitary gonadotrope. Using Cre/Lox technology, we deleted the Kiss1r gene in pituitary gonadotropes (PKiRKO). PKiRKO males have normal genital development (anogenital distance WT: 19.1 ± 0.4 vs. PKiRKO: 18.5 ± 0.4 mm), puberty onset, testes cell structure on gross histology, normal testes size, and fertility. PKiRKO males showed significantly decreased serum FSH levels compared to WT males (5.6 ± 1.9 vs. 10.2 ± 1.8 ng/ml) with comparable LH (1.1 ± 0.2 vs. 1.8 ± 0.4 ng/ml) and testosterone levels (351.8 ± 213.0 vs. 342.2 ± 183.0 ng/dl). PKiRKO females have normal puberty onset, cyclicity, LH and FSH levels and fertility. Overall, these findings indicate that absence of pituitary Kiss1r reduces FSH levels in male mice without affecting testis function. PKiRKO mice have normal reproductive function in both males and females. |
| first_indexed | 2024-04-14T05:44:54Z |
| format | Article |
| id | doaj.art-64888983245946e0ab8726a8a2e58ed4 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-14T05:44:54Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-64888983245946e0ab8726a8a2e58ed42022-12-22T02:09:20ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-04-011310.3389/fphys.2022.868593868593Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin ReceptorYaping Ma0Yaping Ma1Olubusayo Awe2Sally Radovick3Xiaofeng Yang4Sara Divall5Andrew Wolfe6Sheng Wu7Sheng Wu8Sheng Wu9Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Pediatrics, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Cellular and Molecular Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Pediatrics, Rutgers University Medical School, New Brunswick, NJ, United StatesDepartment of Cardiovascular Sciences, Center for Metabolic Disease Research, Temple University School of Medicine, Philadelphia, PA, United StatesDepartment of Pediatrics, University of Washington, Seattle’s Children’s Hospital, Seattle, United StatesDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Cellular and Molecular Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Cardiovascular Sciences, Center for Metabolic Disease Research, Temple University School of Medicine, Philadelphia, PA, United StatesThe kisspeptin receptor, crucial for hypothalamic control of puberty and reproduction, is also present in the pituitary gland. Its role in the pituitary gland is not defined. Kisspeptin signaling via the Kiss1r could potentially regulate reproductive function at the level of pituitary gonadotrope. Using Cre/Lox technology, we deleted the Kiss1r gene in pituitary gonadotropes (PKiRKO). PKiRKO males have normal genital development (anogenital distance WT: 19.1 ± 0.4 vs. PKiRKO: 18.5 ± 0.4 mm), puberty onset, testes cell structure on gross histology, normal testes size, and fertility. PKiRKO males showed significantly decreased serum FSH levels compared to WT males (5.6 ± 1.9 vs. 10.2 ± 1.8 ng/ml) with comparable LH (1.1 ± 0.2 vs. 1.8 ± 0.4 ng/ml) and testosterone levels (351.8 ± 213.0 vs. 342.2 ± 183.0 ng/dl). PKiRKO females have normal puberty onset, cyclicity, LH and FSH levels and fertility. Overall, these findings indicate that absence of pituitary Kiss1r reduces FSH levels in male mice without affecting testis function. PKiRKO mice have normal reproductive function in both males and females.https://www.frontiersin.org/articles/10.3389/fphys.2022.868593/fullkisspeptin receptorGnRHpituitaryreproductionLHFSH |
| spellingShingle | Yaping Ma Yaping Ma Olubusayo Awe Sally Radovick Xiaofeng Yang Sara Divall Andrew Wolfe Sheng Wu Sheng Wu Sheng Wu Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor Frontiers in Physiology kisspeptin receptor GnRH pituitary reproduction LH FSH |
| title | Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor |
| title_full | Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor |
| title_fullStr | Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor |
| title_full_unstemmed | Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor |
| title_short | Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor |
| title_sort | lower fsh with normal fertility in male mice lacking gonadotroph kisspeptin receptor |
| topic | kisspeptin receptor GnRH pituitary reproduction LH FSH |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.868593/full |
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