Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
As a gas signal molecule, hydrogen sulfide (H<sub>2</sub>S) can enhance plant stress resistance. Here, cucumber (<i>Cucumis sativus</i> ‘Xinchun NO. 4’) explants were used to investigate the role of H<sub>2</sub>S in adventitious root development under salt stress...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/7/935 |
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| author | Yayu Liu Lijuan Wei Li Feng Meiling Zhang Dongliang Hu Jianzhong Tie Weibiao Liao |
| author_facet | Yayu Liu Lijuan Wei Li Feng Meiling Zhang Dongliang Hu Jianzhong Tie Weibiao Liao |
| author_sort | Yayu Liu |
| collection | DOAJ |
| description | As a gas signal molecule, hydrogen sulfide (H<sub>2</sub>S) can enhance plant stress resistance. Here, cucumber (<i>Cucumis sativus</i> ‘Xinchun NO. 4’) explants were used to investigate the role of H<sub>2</sub>S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produced moderate salt stress. The 100 µM sodium hydrosulfide (NaHS) treatment, a H<sub>2</sub>S donor, increased root number and root length by 38.37% and 66.75%, respectively, indicating that H<sub>2</sub>S effectively promoted the occurrence of adventitious roots in cucumber explants under salt stress. The results show that under salt stress, NaHS treatment reduced free proline content and increased the soluble sugar and soluble protein content during rooting. Meanwhile, NaHS treatment enhanced the activities of antioxidant enzymes [peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], increased the content of ascorbic (ASA) and glutathione (GSH), reduced the content of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the rate of superoxide radical (O<sup>2−</sup>) production, and decreased relative electrical conductivity (REC) and the content of malondialdehyde (MDA). However, the NaHS scavenger hypotaurine (HT) reversed the above effects of NaHS under salt stress. In summary, H<sub>2</sub>S promoted adventitious root development under salt stress through regulating osmotic substance content and enhancing antioxidant ability in explants. |
| first_indexed | 2024-03-09T11:31:32Z |
| format | Article |
| id | doaj.art-6488c0c4a13b4cc1bd9e7fdce32bc72f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:31:32Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6488c0c4a13b4cc1bd9e7fdce32bc72f2023-11-30T23:51:04ZengMDPI AGPlants2223-77472022-03-0111793510.3390/plants11070935Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant AbilityYayu Liu0Lijuan Wei1Li Feng2Meiling Zhang3Dongliang Hu4Jianzhong Tie5Weibiao Liao6College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaAs a gas signal molecule, hydrogen sulfide (H<sub>2</sub>S) can enhance plant stress resistance. Here, cucumber (<i>Cucumis sativus</i> ‘Xinchun NO. 4’) explants were used to investigate the role of H<sub>2</sub>S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produced moderate salt stress. The 100 µM sodium hydrosulfide (NaHS) treatment, a H<sub>2</sub>S donor, increased root number and root length by 38.37% and 66.75%, respectively, indicating that H<sub>2</sub>S effectively promoted the occurrence of adventitious roots in cucumber explants under salt stress. The results show that under salt stress, NaHS treatment reduced free proline content and increased the soluble sugar and soluble protein content during rooting. Meanwhile, NaHS treatment enhanced the activities of antioxidant enzymes [peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], increased the content of ascorbic (ASA) and glutathione (GSH), reduced the content of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the rate of superoxide radical (O<sup>2−</sup>) production, and decreased relative electrical conductivity (REC) and the content of malondialdehyde (MDA). However, the NaHS scavenger hypotaurine (HT) reversed the above effects of NaHS under salt stress. In summary, H<sub>2</sub>S promoted adventitious root development under salt stress through regulating osmotic substance content and enhancing antioxidant ability in explants.https://www.mdpi.com/2223-7747/11/7/935hydrogen sulfideosmotic substancesantioxidant systemlipid peroxidationadventitious root development |
| spellingShingle | Yayu Liu Lijuan Wei Li Feng Meiling Zhang Dongliang Hu Jianzhong Tie Weibiao Liao Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability Plants hydrogen sulfide osmotic substances antioxidant system lipid peroxidation adventitious root development |
| title | Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability |
| title_full | Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability |
| title_fullStr | Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability |
| title_full_unstemmed | Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability |
| title_short | Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability |
| title_sort | hydrogen sulfide promotes adventitious root development in cucumber under salt stress by enhancing antioxidant ability |
| topic | hydrogen sulfide osmotic substances antioxidant system lipid peroxidation adventitious root development |
| url | https://www.mdpi.com/2223-7747/11/7/935 |
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