Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance
Abstract Diarrhea is characterized by alterations in the gut microbiota, metabolites, and host response to these changes. Studies have focused on the role of commensal bacteria in diarrhea; however, the effect of fungi on its pathogenesis remains unexplored. Here, using post-weaned piglets with or w...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-023-00433-0 |
| _version_ | 1827635402964467712 |
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| author | Xihong Zhou Yiwen He Jingqing Chen Xia Xiong Jie Yin Jing Liang Can Peng Chunxia Huang Guiping Guan Yulong Yin |
| author_facet | Xihong Zhou Yiwen He Jingqing Chen Xia Xiong Jie Yin Jing Liang Can Peng Chunxia Huang Guiping Guan Yulong Yin |
| author_sort | Xihong Zhou |
| collection | DOAJ |
| description | Abstract Diarrhea is characterized by alterations in the gut microbiota, metabolites, and host response to these changes. Studies have focused on the role of commensal bacteria in diarrhea; however, the effect of fungi on its pathogenesis remains unexplored. Here, using post-weaned piglets with or without diarrhea, we found an unexpected decrease in the abundance of Candida tropicalis in diarrheal piglets. We also observed increased accumulation of reactive oxygen species (ROS) and the formation of neutrophil extracellular traps (NETs) in the colonic tissues of diarrheal piglets. Using dectin-1-knockout mice, we found that the over-accumulation of ROS killed C. tropicalis by promoting NET formation, which was dependent on dectin-1. The decreased abundance of C. tropicalis resulted in reduced phosphocholine consumption. Then, colonic phosphocholine accumulation drives water efflux by increasing cAMP levels by activating adenylyl cyclase, which promotes the clearance of pathogenic bacteria. Collectively, we demonstrated that phosphocholine is correlated with colonic C. tropicalis and promotes diarrhea and pathogen clearance. Our results suggest that mycobiota colonizing the colon might be involved in maintaining intestinal metabolic homeostasis through the consumption of certain metabolites. |
| first_indexed | 2024-03-09T15:26:19Z |
| format | Article |
| id | doaj.art-648a5cc3ad8f4b87a556ee1cd6fedb82 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-03-09T15:26:19Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-648a5cc3ad8f4b87a556ee1cd6fedb822023-11-26T12:29:59ZengNature Portfolionpj Biofilms and Microbiomes2055-50082023-09-019111310.1038/s41522-023-00433-0Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearanceXihong Zhou0Yiwen He1Jingqing Chen2Xia Xiong3Jie Yin4Jing Liang5Can Peng6Chunxia Huang7Guiping Guan8Yulong Yin9Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesLaboratory Animal Center of the Academy of Military Medical SciencesKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesCollege of Animal Science and Technology, Hunan Agricultural UniversityKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesSchool of Stomatology, Changsha Medical UniversityCollege of Bioscience & Biotechnology, Hunan Agricultural UniversityKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of SciencesAbstract Diarrhea is characterized by alterations in the gut microbiota, metabolites, and host response to these changes. Studies have focused on the role of commensal bacteria in diarrhea; however, the effect of fungi on its pathogenesis remains unexplored. Here, using post-weaned piglets with or without diarrhea, we found an unexpected decrease in the abundance of Candida tropicalis in diarrheal piglets. We also observed increased accumulation of reactive oxygen species (ROS) and the formation of neutrophil extracellular traps (NETs) in the colonic tissues of diarrheal piglets. Using dectin-1-knockout mice, we found that the over-accumulation of ROS killed C. tropicalis by promoting NET formation, which was dependent on dectin-1. The decreased abundance of C. tropicalis resulted in reduced phosphocholine consumption. Then, colonic phosphocholine accumulation drives water efflux by increasing cAMP levels by activating adenylyl cyclase, which promotes the clearance of pathogenic bacteria. Collectively, we demonstrated that phosphocholine is correlated with colonic C. tropicalis and promotes diarrhea and pathogen clearance. Our results suggest that mycobiota colonizing the colon might be involved in maintaining intestinal metabolic homeostasis through the consumption of certain metabolites.https://doi.org/10.1038/s41522-023-00433-0 |
| spellingShingle | Xihong Zhou Yiwen He Jingqing Chen Xia Xiong Jie Yin Jing Liang Can Peng Chunxia Huang Guiping Guan Yulong Yin Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance npj Biofilms and Microbiomes |
| title | Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance |
| title_full | Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance |
| title_fullStr | Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance |
| title_full_unstemmed | Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance |
| title_short | Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance |
| title_sort | colonic phosphocholine is correlated with candida tropicalis and promotes diarrhea and pathogen clearance |
| url | https://doi.org/10.1038/s41522-023-00433-0 |
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