A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells.
Inside the adenovirus virion, the genome forms a chromatin-like structure with viral basic core proteins. Core protein VII is the major DNA binding protein and was shown to remain associated with viral genomes upon virus entry even after nuclear delivery. It has been suggested that protein VII plays...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4557953?pdf=render |
| _version_ | 1819032122603601920 |
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| author | Tetsuro Komatsu Denis Dacheux Florian Kreppel Kyosuke Nagata Harald Wodrich |
| author_facet | Tetsuro Komatsu Denis Dacheux Florian Kreppel Kyosuke Nagata Harald Wodrich |
| author_sort | Tetsuro Komatsu |
| collection | DOAJ |
| description | Inside the adenovirus virion, the genome forms a chromatin-like structure with viral basic core proteins. Core protein VII is the major DNA binding protein and was shown to remain associated with viral genomes upon virus entry even after nuclear delivery. It has been suggested that protein VII plays a regulatory role in viral gene expression and is a functional component of viral chromatin complexes in host cells. As such, protein VII could be used as a maker to track adenoviral chromatin complexes in vivo. In this study, we characterize a new monoclonal antibody against protein VII that stains incoming viral chromatin complexes following nuclear import. Furthermore, we describe the development of a novel imaging system that uses Template Activating Factor-I (TAF-I/SET), a cellular chromatin protein tightly bound to protein VII upon infection. This setup allows us not only to rapidly visualize protein VII foci in fixed cells but also to monitor their movement in living cells. These powerful tools can provide novel insights into the spatio-temporal regulation of incoming adenoviral chromatin complexes. |
| first_indexed | 2024-12-21T06:56:56Z |
| format | Article |
| id | doaj.art-648b100e962b47958a01e6dd0122a319 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T06:56:56Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-648b100e962b47958a01e6dd0122a3192022-12-21T19:12:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013710210.1371/journal.pone.0137102A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells.Tetsuro KomatsuDenis DacheuxFlorian KreppelKyosuke NagataHarald WodrichInside the adenovirus virion, the genome forms a chromatin-like structure with viral basic core proteins. Core protein VII is the major DNA binding protein and was shown to remain associated with viral genomes upon virus entry even after nuclear delivery. It has been suggested that protein VII plays a regulatory role in viral gene expression and is a functional component of viral chromatin complexes in host cells. As such, protein VII could be used as a maker to track adenoviral chromatin complexes in vivo. In this study, we characterize a new monoclonal antibody against protein VII that stains incoming viral chromatin complexes following nuclear import. Furthermore, we describe the development of a novel imaging system that uses Template Activating Factor-I (TAF-I/SET), a cellular chromatin protein tightly bound to protein VII upon infection. This setup allows us not only to rapidly visualize protein VII foci in fixed cells but also to monitor their movement in living cells. These powerful tools can provide novel insights into the spatio-temporal regulation of incoming adenoviral chromatin complexes.http://europepmc.org/articles/PMC4557953?pdf=render |
| spellingShingle | Tetsuro Komatsu Denis Dacheux Florian Kreppel Kyosuke Nagata Harald Wodrich A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. PLoS ONE |
| title | A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. |
| title_full | A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. |
| title_fullStr | A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. |
| title_full_unstemmed | A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. |
| title_short | A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells. |
| title_sort | method for visualization of incoming adenovirus chromatin complexes in fixed and living cells |
| url | http://europepmc.org/articles/PMC4557953?pdf=render |
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