In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam
Hoa Binh province is one of the best places for orchids in Vietnam. The climate and environment of Hoa Binh province are favorable for the development of orchids, especially rare indigenous ones. <i>Dendrobium anosmum</i> Lindl., which stands out because of the unique fragrance and color...
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MDPI AG
2022-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/2/324 |
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| author | Hai T. Nguyen Son T. Dinh Thao T. Ninh Hue T. Nong Tam T. T. Dang Quyet V. Khuat Anh T. P. Dang My T. Ly Rima N. Kirakosyan Elena A. Kalashnikova |
| author_facet | Hai T. Nguyen Son T. Dinh Thao T. Ninh Hue T. Nong Tam T. T. Dang Quyet V. Khuat Anh T. P. Dang My T. Ly Rima N. Kirakosyan Elena A. Kalashnikova |
| author_sort | Hai T. Nguyen |
| collection | DOAJ |
| description | Hoa Binh province is one of the best places for orchids in Vietnam. The climate and environment of Hoa Binh province are favorable for the development of orchids, especially rare indigenous ones. <i>Dendrobium anosmum</i> Lindl., which stands out because of the unique fragrance and colors, is one of the most popular varieties in Hoa Binh province. To meet the increasing demands of the industrial market as well as to contribute to the preservation and development of genetic resources of <i>Dendrobium</i> sp. in Hoa Binh province, propagating <i>D. anosmum</i> Lindl. is a crucial step. Plant tissue culture, which has been applied to improve reproducibility of orchids for many years, is still an effective method, especially for large-scale propagation. Studies on in vitro propagation of <i>D. anosmum</i> Lindl. from Hoa Binh province showed that growth regulators (BA, kinetin, α-NAA) did not have a significant effect on protocorm initiation because <i>D. anosmum</i> Lind. from Hoa Binh province already has a high rate of regeneration. However, MS medium + 1.0 mg/L kinetin + 0.5 mg/L α-NAA + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the optimal medium to increase shoot length. The MS medium + 1.0 g activated charcoal + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the most suitable medium for shoot growth—after 6 weeks of culture, the average shoot length was 1.09 cm, the average number of leaves was 6.13, the average number of roots was 3.17, and the average root length was 1.11 cm—about 3.3, 4.17, 3.41, and 1.67 times higher, respectively, than in the control (without activated charcoal). |
| first_indexed | 2024-03-09T22:51:19Z |
| format | Article |
| id | doaj.art-648c3dd817894ba69223a8dfa0165835 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T22:51:19Z |
| publishDate | 2022-01-01 |
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| series | Agronomy |
| spelling | doaj.art-648c3dd817894ba69223a8dfa01658352023-11-23T18:20:01ZengMDPI AGAgronomy2073-43952022-01-0112232410.3390/agronomy12020324In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in VietnamHai T. Nguyen0Son T. Dinh1Thao T. Ninh2Hue T. Nong3Tam T. T. Dang4Quyet V. Khuat5Anh T. P. Dang6My T. Ly7Rima N. Kirakosyan8Elena A. Kalashnikova9Department of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Biotechnology, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya, 49, 127550 Moscow, RussiaDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Plant Biotechnology, Vietnam National University of Agriculture, Gia lam, Hanoi 12406, VietnamDepartment of Biotechnology, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya, 49, 127550 Moscow, RussiaDepartment of Biotechnology, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya, 49, 127550 Moscow, RussiaHoa Binh province is one of the best places for orchids in Vietnam. The climate and environment of Hoa Binh province are favorable for the development of orchids, especially rare indigenous ones. <i>Dendrobium anosmum</i> Lindl., which stands out because of the unique fragrance and colors, is one of the most popular varieties in Hoa Binh province. To meet the increasing demands of the industrial market as well as to contribute to the preservation and development of genetic resources of <i>Dendrobium</i> sp. in Hoa Binh province, propagating <i>D. anosmum</i> Lindl. is a crucial step. Plant tissue culture, which has been applied to improve reproducibility of orchids for many years, is still an effective method, especially for large-scale propagation. Studies on in vitro propagation of <i>D. anosmum</i> Lindl. from Hoa Binh province showed that growth regulators (BA, kinetin, α-NAA) did not have a significant effect on protocorm initiation because <i>D. anosmum</i> Lind. from Hoa Binh province already has a high rate of regeneration. However, MS medium + 1.0 mg/L kinetin + 0.5 mg/L α-NAA + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the optimal medium to increase shoot length. The MS medium + 1.0 g activated charcoal + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the most suitable medium for shoot growth—after 6 weeks of culture, the average shoot length was 1.09 cm, the average number of leaves was 6.13, the average number of roots was 3.17, and the average root length was 1.11 cm—about 3.3, 4.17, 3.41, and 1.67 times higher, respectively, than in the control (without activated charcoal).https://www.mdpi.com/2073-4395/12/2/324in vitro<i>Dendrobium anosmum</i>Vietnammicropropagationprotocorm |
| spellingShingle | Hai T. Nguyen Son T. Dinh Thao T. Ninh Hue T. Nong Tam T. T. Dang Quyet V. Khuat Anh T. P. Dang My T. Ly Rima N. Kirakosyan Elena A. Kalashnikova In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam Agronomy in vitro <i>Dendrobium anosmum</i> Vietnam micropropagation protocorm |
| title | In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam |
| title_full | In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam |
| title_fullStr | In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam |
| title_full_unstemmed | In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam |
| title_short | In Vitro Propagation of the <i>Dendrobium anosmum</i> Lindl. Collected in Vietnam |
| title_sort | in vitro propagation of the i dendrobium anosmum i lindl collected in vietnam |
| topic | in vitro <i>Dendrobium anosmum</i> Vietnam micropropagation protocorm |
| url | https://www.mdpi.com/2073-4395/12/2/324 |
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