In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine
Brucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due to serious economic and medical consequences of this disease, various efforts have been made to prevent the infection through the use of recombinant vaccines based on Brucella outer membrane protein (OM...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine and Serum Research Institute
2016-03-01
|
| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | http://www.archrazi.com/article_105996_10696.html |
| _version_ | 1818230057418620928 |
|---|---|
| author | Tahmoorespur, M. Sekhavati, M.H. Yousefi, S. Abbassi-Daloii, T. Azghandi, M. Akbari, R. |
| author_facet | Tahmoorespur, M. Sekhavati, M.H. Yousefi, S. Abbassi-Daloii, T. Azghandi, M. Akbari, R. |
| author_sort | Tahmoorespur, M. |
| collection | DOAJ |
| description | Brucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due to serious economic and medical consequences of this disease, various efforts have been made to prevent the infection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP) antigens. The objectives of the present study were cloning, sequencing and epitope prediction of Omp25 and BLS genes as two major Brucella melitensis antigens. The full-length open reading frame (ORF) of Omp25 and BLS genes were amplified and cloned into pTZ57R/T vector. Phylogenetic analysis of sequenced genes showed that both genes were nearly similar in different Brucella species. Several online prediction softwares were used to predict B and T-cells epitopes, secondary and tertiary structures, antigenicity ability and enzymatic degradation sites. Bioinformatic tools used in the current study were confirmed by the results of three different experimental epitope predictions. Bioinformatic analysis identified five and two B-cell and two and one T-cell epitopes for Omp25 and BLS antigens, respectively. Finally, according to the antigenicity ability and proteosomal recognition site common B and T-cell epitope was predicted for Omp25 (154-162 amino acids) and BLS (37-48 and 119-139 amino acids). Results of this study might be useful for recombinant vaccine development. |
| first_indexed | 2024-12-12T10:28:27Z |
| format | Article |
| id | doaj.art-648c5a1651f2421ab5af01bab748ced8 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-12T10:28:27Z |
| publishDate | 2016-03-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-648c5a1651f2421ab5af01bab748ced82022-12-22T00:27:24ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722016-03-017113542In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccineTahmoorespur, M.0 Sekhavati, M.H.1Yousefi, S.2 Abbassi-Daloii, T.3 Azghandi, M.4Akbari, R.5Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Animal Science, Ferdowsi University of Mashhad, Mashhad, IranBrucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due to serious economic and medical consequences of this disease, various efforts have been made to prevent the infection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP) antigens. The objectives of the present study were cloning, sequencing and epitope prediction of Omp25 and BLS genes as two major Brucella melitensis antigens. The full-length open reading frame (ORF) of Omp25 and BLS genes were amplified and cloned into pTZ57R/T vector. Phylogenetic analysis of sequenced genes showed that both genes were nearly similar in different Brucella species. Several online prediction softwares were used to predict B and T-cells epitopes, secondary and tertiary structures, antigenicity ability and enzymatic degradation sites. Bioinformatic tools used in the current study were confirmed by the results of three different experimental epitope predictions. Bioinformatic analysis identified five and two B-cell and two and one T-cell epitopes for Omp25 and BLS antigens, respectively. Finally, according to the antigenicity ability and proteosomal recognition site common B and T-cell epitope was predicted for Omp25 (154-162 amino acids) and BLS (37-48 and 119-139 amino acids). Results of this study might be useful for recombinant vaccine development.http://www.archrazi.com/article_105996_10696.htmlBrucella melitensisOmp25BLSBioinformatics analysisRecombinant vaccine |
| spellingShingle | Tahmoorespur, M. Sekhavati, M.H. Yousefi, S. Abbassi-Daloii, T. Azghandi, M. Akbari, R. In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine Archives of Razi Institute Brucella melitensis Omp25 BLS Bioinformatics analysis Recombinant vaccine |
| title | In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine |
| title_full | In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine |
| title_fullStr | In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine |
| title_full_unstemmed | In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine |
| title_short | In silico analysis of Omp25 and BLS Brucella melitensis antigens for designing subunit vaccine |
| title_sort | in silico analysis of omp25 and bls brucella melitensis antigens for designing subunit vaccine |
| topic | Brucella melitensis Omp25 BLS Bioinformatics analysis Recombinant vaccine |
| url | http://www.archrazi.com/article_105996_10696.html |
| work_keys_str_mv | AT tahmoorespurm insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine AT sekhavatimh insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine AT yousefis insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine AT abbassidaloiit insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine AT azghandim insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine AT akbarir insilicoanalysisofomp25andblsbrucellamelitensisantigensfordesigningsubunitvaccine |