Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules
Accurate and reliable detection of biological molecules such as nucleic acids, proteins, and small molecules is essential for the diagnosis and treatment of diseases. While simple homogeneous assays have been developed and are widely used for detecting nucleic acids, non-nucleic acid molecules such...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1188313/full |
| _version_ | 1827913064175894528 |
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| author | Ye Seop Park Sunjoo Choi Hee Ju Jang Tae Hyeon Yoo Tae Hyeon Yoo |
| author_facet | Ye Seop Park Sunjoo Choi Hee Ju Jang Tae Hyeon Yoo Tae Hyeon Yoo |
| author_sort | Ye Seop Park |
| collection | DOAJ |
| description | Accurate and reliable detection of biological molecules such as nucleic acids, proteins, and small molecules is essential for the diagnosis and treatment of diseases. While simple homogeneous assays have been developed and are widely used for detecting nucleic acids, non-nucleic acid molecules such as proteins and small molecules are usually analyzed using methods that require time-consuming procedures and highly trained personnel. Recently, methods using proximity-enhanced reactions (PERs) have been developed for detecting non-nucleic acids. These reactions can be conducted in a homogeneous liquid phase via a single-step procedure. Herein, we review three assays based on PERs for the detection of non-nucleic acid molecules: proximity ligation assay, proximity extension assay, and proximity proteolysis assay. |
| first_indexed | 2024-03-13T02:25:05Z |
| format | Article |
| id | doaj.art-6493bb0f93474827b1b4aba7537ad4d7 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T02:25:05Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-6493bb0f93474827b1b4aba7537ad4d72023-06-30T05:05:15ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-06-011110.3389/fbioe.2023.11883131188313Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid moleculesYe Seop Park0Sunjoo Choi1Hee Ju Jang2Tae Hyeon Yoo3Tae Hyeon Yoo4Department of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon, Republic of KoreaDepartment of Applied Chemistry and Biological Engineering, Ajou University, Suwon, Republic of KoreaAccurate and reliable detection of biological molecules such as nucleic acids, proteins, and small molecules is essential for the diagnosis and treatment of diseases. While simple homogeneous assays have been developed and are widely used for detecting nucleic acids, non-nucleic acid molecules such as proteins and small molecules are usually analyzed using methods that require time-consuming procedures and highly trained personnel. Recently, methods using proximity-enhanced reactions (PERs) have been developed for detecting non-nucleic acids. These reactions can be conducted in a homogeneous liquid phase via a single-step procedure. Herein, we review three assays based on PERs for the detection of non-nucleic acid molecules: proximity ligation assay, proximity extension assay, and proximity proteolysis assay.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1188313/fullbiosensorproximity-enhanced reactionproximity ligation assayproximity extension assayproximity proteolysis assay |
| spellingShingle | Ye Seop Park Sunjoo Choi Hee Ju Jang Tae Hyeon Yoo Tae Hyeon Yoo Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules Frontiers in Bioengineering and Biotechnology biosensor proximity-enhanced reaction proximity ligation assay proximity extension assay proximity proteolysis assay |
| title | Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules |
| title_full | Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules |
| title_fullStr | Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules |
| title_full_unstemmed | Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules |
| title_short | Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules |
| title_sort | assay methods based on proximity enhanced reactions for detecting non nucleic acid molecules |
| topic | biosensor proximity-enhanced reaction proximity ligation assay proximity extension assay proximity proteolysis assay |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1188313/full |
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