Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis
The excessive expression of reactive oxygen species is closely connected to many diseases. Considerable studies have demonstrated dandelion as well as its ingredients exhibited antioxidant activity. However, specific material basis reflecting the antioxidant activity has not been comprehensively inv...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2632 |
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| author | Ziru Liu Jiameng Qu Fan Ke Haotian Zhang Yiwen Zhang Qian Zhang Qing Li Kaishun Bi Huarong Xu |
| author_facet | Ziru Liu Jiameng Qu Fan Ke Haotian Zhang Yiwen Zhang Qian Zhang Qing Li Kaishun Bi Huarong Xu |
| author_sort | Ziru Liu |
| collection | DOAJ |
| description | The excessive expression of reactive oxygen species is closely connected to many diseases. Considerable studies have demonstrated dandelion as well as its ingredients exhibited antioxidant activity. However, specific material basis reflecting the antioxidant activity has not been comprehensively investigated. In this study, a spectrum–effect relationship study on dandelion between fingerprinting and antioxidant activity was analyzed in detail, while a UHPLC quantification method developed and completely validated for simultaneous determination of active ingredients in dandelion. With the establishment of dandelion fingerprints of different regions, 24 common peaks were characterized. The classic FRAP method and ABTS methods were then used to detect their antioxidant activity. Partial least squares regression analysis, bivariate correlation analysis and grey correlation method were used to accomplish the spectrum–effect relationship. Eventually, the ingredients with antioxidant activity which could be considered as candidate quality markers of dandelion were discovered through spectrum–effect relationship analysis. The six compounds including caftaric acid, chlorogenic acid, caffeic acid, chicoric acid, isochlorogenic acid A, and isochlorogenic acid C were quantitatively determined. The developed UHPLC assay method was accurate, precise, and reliable. The study has elucidated the antioxidant material basis of dandelion and provided a scientific basis for the quality control of dandelion. |
| first_indexed | 2024-03-10T03:55:35Z |
| format | Article |
| id | doaj.art-64945d1b91e04e5ca84e14edd4ef5b6a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:55:35Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-64945d1b91e04e5ca84e14edd4ef5b6a2023-11-23T08:47:21ZengMDPI AGMolecules1420-30492022-04-01279263210.3390/molecules27092632Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical AnalysisZiru Liu0Jiameng Qu1Fan Ke2Haotian Zhang3Yiwen Zhang4Qian Zhang5Qing Li6Kaishun Bi7Huarong Xu8National and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Traditional Chinese Material Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaNational and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaThe excessive expression of reactive oxygen species is closely connected to many diseases. Considerable studies have demonstrated dandelion as well as its ingredients exhibited antioxidant activity. However, specific material basis reflecting the antioxidant activity has not been comprehensively investigated. In this study, a spectrum–effect relationship study on dandelion between fingerprinting and antioxidant activity was analyzed in detail, while a UHPLC quantification method developed and completely validated for simultaneous determination of active ingredients in dandelion. With the establishment of dandelion fingerprints of different regions, 24 common peaks were characterized. The classic FRAP method and ABTS methods were then used to detect their antioxidant activity. Partial least squares regression analysis, bivariate correlation analysis and grey correlation method were used to accomplish the spectrum–effect relationship. Eventually, the ingredients with antioxidant activity which could be considered as candidate quality markers of dandelion were discovered through spectrum–effect relationship analysis. The six compounds including caftaric acid, chlorogenic acid, caffeic acid, chicoric acid, isochlorogenic acid A, and isochlorogenic acid C were quantitatively determined. The developed UHPLC assay method was accurate, precise, and reliable. The study has elucidated the antioxidant material basis of dandelion and provided a scientific basis for the quality control of dandelion.https://www.mdpi.com/1420-3049/27/9/2632dandelionantioxidant activityspectrum–effect relationshipquantitative analysisquality control |
| spellingShingle | Ziru Liu Jiameng Qu Fan Ke Haotian Zhang Yiwen Zhang Qian Zhang Qing Li Kaishun Bi Huarong Xu Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis Molecules dandelion antioxidant activity spectrum–effect relationship quantitative analysis quality control |
| title | Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis |
| title_full | Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis |
| title_fullStr | Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis |
| title_full_unstemmed | Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis |
| title_short | Material Basis Elucidation and Quantification of Dandelion through Spectrum–Effect Relationship Study between UHPLC Fingerprint and Antioxidant Activity via Multivariate Statistical Analysis |
| title_sort | material basis elucidation and quantification of dandelion through spectrum effect relationship study between uhplc fingerprint and antioxidant activity via multivariate statistical analysis |
| topic | dandelion antioxidant activity spectrum–effect relationship quantitative analysis quality control |
| url | https://www.mdpi.com/1420-3049/27/9/2632 |
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