Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus)
The current study was conducted to determine the effects of dietary Radix bupleuri root extract (RBE) on the growth performance, muscle composition, immune responses, and microcystin-LR (MC-LR) stress resistance of juvenile red claw crayfish (Cherax quadricarinatus). Crayfish (0.28 ± 0.02 g) were fe...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003617 |
| _version_ | 1797388812405440512 |
|---|---|
| author | Yao-Peng Lu Pei-Hua Zheng Ze-Long Zhang Jun-Tao Li Jia-Jun Li Teng Li Xiao Wang Jia-Rui Xu Dong-Mei Wang Jian-An Xian Xiu-Xia Zhang |
| author_facet | Yao-Peng Lu Pei-Hua Zheng Ze-Long Zhang Jun-Tao Li Jia-Jun Li Teng Li Xiao Wang Jia-Rui Xu Dong-Mei Wang Jian-An Xian Xiu-Xia Zhang |
| author_sort | Yao-Peng Lu |
| collection | DOAJ |
| description | The current study was conducted to determine the effects of dietary Radix bupleuri root extract (RBE) on the growth performance, muscle composition, immune responses, and microcystin-LR (MC-LR) stress resistance of juvenile red claw crayfish (Cherax quadricarinatus). Crayfish (0.28 ± 0.02 g) were fed experimental diets containing graded RBE levels as 0 (Diet 1), 0.2 (Diet 2), 0.4 (Diet 3), 0.6 (Diet 4), 0.8 (Diet 5) and 1.6 (Diet 6) g/kg RBE for 8 weeks. The results showed that specific growth rate (SGR) and weight gain rate (WGR) were significantly increased in the Diet 4 and Diet 5 groups. The content of muscle crude protein was significantly increased in the Diet 4 and Diet 5 groups, and the crude lipid content was significantly decreased in the Diet 5 group. Dietary RBE did not alter the structure of the hepatopancreas. Total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activities in hepatopancreas and plasma were significantly increased in Diet 4 and Diet 5 groups compared to the control group, while the malondialdehyde (MDA) content in hepatopancreas and plasma was significantly decreased in the Diet 5 group. Plasma alkaline phosphatase (AKP) activity was significantly higher in the Diet 3, Diet 4, Diet 5 and Diet 6 groups. Hepatopancreas and plasma PO activities were significantly increased in Diet 4 and Diet 5 groups. The mRNA levels of extracellular copper/zinc superoxide dismutase (ecCu, Zn-SOD), hemocyanin, C-type lysozyme (C-LZM), GPx, and selenium-dependent glutathione peroxidase (Se-GPx) were significantly up-regulated in the hepatopancreas of crayfish fed Diet 5 than those of the control group. After MC-LR challenge, the survival rates of crayfish in the Diet 4 and Diet 5 groups were significantly higher than the control group. The findings suggest that 0.6–0.8 g/kg of RBE can improve growth and muscle composition and enhance immunity and microcystin-LR resistance. |
| first_indexed | 2024-03-08T22:46:15Z |
| format | Article |
| id | doaj.art-6497c8ea036b40d0a931724072fcaf1f |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:15Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-6497c8ea036b40d0a931724072fcaf1f2023-12-17T06:39:51ZengElsevierAquaculture Reports2352-51342023-12-0133101822Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus)Yao-Peng Lu0Pei-Hua Zheng1Ze-Long Zhang2Jun-Tao Li3Jia-Jun Li4Teng Li5Xiao Wang6Jia-Rui Xu7Dong-Mei Wang8Jian-An Xian9Xiu-Xia Zhang10Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaHainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaCorresponding authors.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaCorresponding authors.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences & Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, ChinaThe current study was conducted to determine the effects of dietary Radix bupleuri root extract (RBE) on the growth performance, muscle composition, immune responses, and microcystin-LR (MC-LR) stress resistance of juvenile red claw crayfish (Cherax quadricarinatus). Crayfish (0.28 ± 0.02 g) were fed experimental diets containing graded RBE levels as 0 (Diet 1), 0.2 (Diet 2), 0.4 (Diet 3), 0.6 (Diet 4), 0.8 (Diet 5) and 1.6 (Diet 6) g/kg RBE for 8 weeks. The results showed that specific growth rate (SGR) and weight gain rate (WGR) were significantly increased in the Diet 4 and Diet 5 groups. The content of muscle crude protein was significantly increased in the Diet 4 and Diet 5 groups, and the crude lipid content was significantly decreased in the Diet 5 group. Dietary RBE did not alter the structure of the hepatopancreas. Total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activities in hepatopancreas and plasma were significantly increased in Diet 4 and Diet 5 groups compared to the control group, while the malondialdehyde (MDA) content in hepatopancreas and plasma was significantly decreased in the Diet 5 group. Plasma alkaline phosphatase (AKP) activity was significantly higher in the Diet 3, Diet 4, Diet 5 and Diet 6 groups. Hepatopancreas and plasma PO activities were significantly increased in Diet 4 and Diet 5 groups. The mRNA levels of extracellular copper/zinc superoxide dismutase (ecCu, Zn-SOD), hemocyanin, C-type lysozyme (C-LZM), GPx, and selenium-dependent glutathione peroxidase (Se-GPx) were significantly up-regulated in the hepatopancreas of crayfish fed Diet 5 than those of the control group. After MC-LR challenge, the survival rates of crayfish in the Diet 4 and Diet 5 groups were significantly higher than the control group. The findings suggest that 0.6–0.8 g/kg of RBE can improve growth and muscle composition and enhance immunity and microcystin-LR resistance.http://www.sciencedirect.com/science/article/pii/S2352513423003617Radix bupleuriGrowthAntioxidantsImmune parametersAquaculture feeding |
| spellingShingle | Yao-Peng Lu Pei-Hua Zheng Ze-Long Zhang Jun-Tao Li Jia-Jun Li Teng Li Xiao Wang Jia-Rui Xu Dong-Mei Wang Jian-An Xian Xiu-Xia Zhang Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) Aquaculture Reports Radix bupleuri Growth Antioxidants Immune parameters Aquaculture feeding |
| title | Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) |
| title_full | Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) |
| title_fullStr | Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) |
| title_full_unstemmed | Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) |
| title_short | Effects of dietary Radix bupleuri root extract on the growth, muscle composition, histology, immune responses and microcystin-LR stress resistance of juvenile red claw crayfish (Cherax quadricarinatus) |
| title_sort | effects of dietary radix bupleuri root extract on the growth muscle composition histology immune responses and microcystin lr stress resistance of juvenile red claw crayfish cherax quadricarinatus |
| topic | Radix bupleuri Growth Antioxidants Immune parameters Aquaculture feeding |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003617 |
| work_keys_str_mv | AT yaopenglu effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT peihuazheng effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT zelongzhang effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT juntaoli effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT jiajunli effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT tengli effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT xiaowang effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT jiaruixu effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT dongmeiwang effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT jiananxian effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus AT xiuxiazhang effectsofdietaryradixbupleurirootextractonthegrowthmusclecompositionhistologyimmuneresponsesandmicrocystinlrstressresistanceofjuvenileredclawcrayfishcheraxquadricarinatus |