Crown gall of grapevine in Slovenia
42 samples of grapevine plants and propagating material were taken in 2 Slovene vine growing districts and tested for the presence of Agrobacterium sp. 31 samples had suspicious symptoms, the other 11 did not have them. The samples were analysed using 4 methods: growth of agrobacteria on 4 semisele...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Ljubljana Press (Založba Univerze v Ljubljani)
2000-03-01
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| Series: | Acta Agriculturae Slovenica |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15824 |
| _version_ | 1797386913145946112 |
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| author | Marta ŠABEC-PARADIŽ Vojko ŠKERLAVAJ |
| author_facet | Marta ŠABEC-PARADIŽ Vojko ŠKERLAVAJ |
| author_sort | Marta ŠABEC-PARADIŽ |
| collection | DOAJ |
| description |
42 samples of grapevine plants and propagating material were taken in 2 Slovene vine growing districts and tested for the presence of Agrobacterium sp. 31 samples had suspicious symptoms, the other 11 did not have them. The samples were analysed using 4 methods: growth of agrobacteria on 4 semiselective media, detection of T-DNA carrying oncogenes tmr, acs and vis/6b by PCR, detection of opines in tumor tissue and pathogenicity testing on tomato and Kalanchoe daigremontiana plants. 20 samples with symptoms were proved to be infected with Agrobacterium vitis by means of molecular method and 19 by detection of opines. A. vitis was also proved in 3 samples without symptoms. 19 isolates induced tumor formation on tested plants. This is the first evidence of A.vitis in Slovenia.
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| first_indexed | 2024-03-08T22:16:16Z |
| format | Article |
| id | doaj.art-649bd6b9d661473cbfec047315fda107 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T22:16:16Z |
| publishDate | 2000-03-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-649bd6b9d661473cbfec047315fda1072023-12-18T22:15:10ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412000-03-01751Crown gall of grapevine in SloveniaMarta ŠABEC-PARADIŽVojko ŠKERLAVAJ 42 samples of grapevine plants and propagating material were taken in 2 Slovene vine growing districts and tested for the presence of Agrobacterium sp. 31 samples had suspicious symptoms, the other 11 did not have them. The samples were analysed using 4 methods: growth of agrobacteria on 4 semiselective media, detection of T-DNA carrying oncogenes tmr, acs and vis/6b by PCR, detection of opines in tumor tissue and pathogenicity testing on tomato and Kalanchoe daigremontiana plants. 20 samples with symptoms were proved to be infected with Agrobacterium vitis by means of molecular method and 19 by detection of opines. A. vitis was also proved in 3 samples without symptoms. 19 isolates induced tumor formation on tested plants. This is the first evidence of A.vitis in Slovenia. https://journals.uni-lj.si/aas/article/view/15824 |
| spellingShingle | Marta ŠABEC-PARADIŽ Vojko ŠKERLAVAJ Crown gall of grapevine in Slovenia Acta Agriculturae Slovenica |
| title | Crown gall of grapevine in Slovenia |
| title_full | Crown gall of grapevine in Slovenia |
| title_fullStr | Crown gall of grapevine in Slovenia |
| title_full_unstemmed | Crown gall of grapevine in Slovenia |
| title_short | Crown gall of grapevine in Slovenia |
| title_sort | crown gall of grapevine in slovenia |
| url | https://journals.uni-lj.si/aas/article/view/15824 |
| work_keys_str_mv | AT martasabecparadiz crowngallofgrapevineinslovenia AT vojkoskerlavaj crowngallofgrapevineinslovenia |