The RNA Viruses in Samples of Endemic Lake Baikal Sponges
Sponges are unusual representatives of the animal kingdom; their viromes, as part of the associated community, began to be studied quite recently, and, accordingly, these studies are gaining momentum. The diversity of viruses in sponges is high, and they most likely play a significant role in the co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/1424-2818/15/7/835 |
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| author | Tatyana V. Butina Igor V. Khanaev Ivan S. Petrushin Artem N. Bondaryuk Olga O. Maikova Yurij S. Bukin |
| author_facet | Tatyana V. Butina Igor V. Khanaev Ivan S. Petrushin Artem N. Bondaryuk Olga O. Maikova Yurij S. Bukin |
| author_sort | Tatyana V. Butina |
| collection | DOAJ |
| description | Sponges are unusual representatives of the animal kingdom; their viromes, as part of the associated community, began to be studied quite recently, and, accordingly, these studies are gaining momentum. The diversity of viruses in sponges is high, and they most likely play a significant role in the composition of the sponge holobiont, especially under stress conditions. The objects of our metagenomic study were RNA viruses of two common endemic species of Baikal sponges, <i>Lubomirskia baikalensis</i> and <i>Baikalospongia bacillifera</i>. As a result of viral RNA sequencing, we were able to identify fragments of viral genomes related to those from the RefSeq NCBI complete viral genome database. Most of the similar genomes belonged to viruses isolated from various invertebrates; some of the scaffolds were related to known plant viruses, and one of them was related to a vertebrate virus. The similarity of the putative proteins of viral scaffolds from the Baikal sponges with proteins of known viruses turned out to be low (20.7–67.3%), indicating the detection of novel viruses. The samples of diseased and visually healthy sponges were clustered separately, suggesting a shift in sponge virome composition during the course of the disease. In a comparative analysis, the viromes of the Baikal and marine sponges differed significantly, demonstrating the influence of the host species, habitat, and geographical location on virome composition in the sponge holobiont. |
| first_indexed | 2024-03-11T01:09:41Z |
| format | Article |
| id | doaj.art-649bd95278d84bc597842f4ade68de1f |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T01:09:41Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-649bd95278d84bc597842f4ade68de1f2023-11-18T18:59:54ZengMDPI AGDiversity1424-28182023-07-0115783510.3390/d15070835The RNA Viruses in Samples of Endemic Lake Baikal SpongesTatyana V. Butina0Igor V. Khanaev1Ivan S. Petrushin2Artem N. Bondaryuk3Olga O. Maikova4Yurij S. Bukin5Limnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLimnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLimnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLimnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLimnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLimnological Institute Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaSponges are unusual representatives of the animal kingdom; their viromes, as part of the associated community, began to be studied quite recently, and, accordingly, these studies are gaining momentum. The diversity of viruses in sponges is high, and they most likely play a significant role in the composition of the sponge holobiont, especially under stress conditions. The objects of our metagenomic study were RNA viruses of two common endemic species of Baikal sponges, <i>Lubomirskia baikalensis</i> and <i>Baikalospongia bacillifera</i>. As a result of viral RNA sequencing, we were able to identify fragments of viral genomes related to those from the RefSeq NCBI complete viral genome database. Most of the similar genomes belonged to viruses isolated from various invertebrates; some of the scaffolds were related to known plant viruses, and one of them was related to a vertebrate virus. The similarity of the putative proteins of viral scaffolds from the Baikal sponges with proteins of known viruses turned out to be low (20.7–67.3%), indicating the detection of novel viruses. The samples of diseased and visually healthy sponges were clustered separately, suggesting a shift in sponge virome composition during the course of the disease. In a comparative analysis, the viromes of the Baikal and marine sponges differed significantly, demonstrating the influence of the host species, habitat, and geographical location on virome composition in the sponge holobiont.https://www.mdpi.com/1424-2818/15/7/835RNA virusesspongesviromeviral diversityinvertebratefreshwater ecosystems |
| spellingShingle | Tatyana V. Butina Igor V. Khanaev Ivan S. Petrushin Artem N. Bondaryuk Olga O. Maikova Yurij S. Bukin The RNA Viruses in Samples of Endemic Lake Baikal Sponges Diversity RNA viruses sponges virome viral diversity invertebrate freshwater ecosystems |
| title | The RNA Viruses in Samples of Endemic Lake Baikal Sponges |
| title_full | The RNA Viruses in Samples of Endemic Lake Baikal Sponges |
| title_fullStr | The RNA Viruses in Samples of Endemic Lake Baikal Sponges |
| title_full_unstemmed | The RNA Viruses in Samples of Endemic Lake Baikal Sponges |
| title_short | The RNA Viruses in Samples of Endemic Lake Baikal Sponges |
| title_sort | rna viruses in samples of endemic lake baikal sponges |
| topic | RNA viruses sponges virome viral diversity invertebrate freshwater ecosystems |
| url | https://www.mdpi.com/1424-2818/15/7/835 |
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