Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions
While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of Protein-Protein Interactions (PPIs) can...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-07-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00237/full |
| _version_ | 1811269995559124992 |
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| author | Katrina eMeyer Matthias eSelbach Matthias eSelbach |
| author_facet | Katrina eMeyer Matthias eSelbach Matthias eSelbach |
| author_sort | Katrina eMeyer |
| collection | DOAJ |
| description | While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of Protein-Protein Interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior. |
| first_indexed | 2024-04-12T21:51:57Z |
| format | Article |
| id | doaj.art-649dd83f40444cfcad3a3fd0f5601b42 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-12T21:51:57Z |
| publishDate | 2015-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-649dd83f40444cfcad3a3fd0f5601b422022-12-22T03:15:26ZengFrontiers Media S.A.Frontiers in Genetics1664-80212015-07-01610.3389/fgene.2015.00237145573Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactionsKatrina eMeyer0Matthias eSelbach1Matthias eSelbach2Max Delbruck Center for Molecular MedicineMax Delbruck Center for Molecular MedicineCharitéWhile the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of Protein-Protein Interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior.http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00237/fullprotein protein interactionstoichiometrymass spectrometry based proteomicsCross-linkingquantiative proteomics |
| spellingShingle | Katrina eMeyer Matthias eSelbach Matthias eSelbach Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions Frontiers in Genetics protein protein interaction stoichiometry mass spectrometry based proteomics Cross-linking quantiative proteomics |
| title | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| title_full | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| title_fullStr | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| title_full_unstemmed | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| title_short | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| title_sort | quantitative affinity purification mass spectrometry a versatile technology to study protein protein interactions |
| topic | protein protein interaction stoichiometry mass spectrometry based proteomics Cross-linking quantiative proteomics |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00237/full |
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