Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages
<i>Vitellaria paradoxa</i> C. F. Gaertn is widely used in African traditional medicine as an anti-inflammatory remedy to treat rheumatism, gastric problems, diarrhea, and dysentery. The phytochemical investigation of the ethyl acetate extract of <i>V. paradoxa</i> stem bark c...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| author | Carmina Sirignano Pascal Nadembega Ferruccio Poli Barbara Romano Giuseppe Lucariello Daniela Rigano Orazio Taglialatela-Scafati |
| author_facet | Carmina Sirignano Pascal Nadembega Ferruccio Poli Barbara Romano Giuseppe Lucariello Daniela Rigano Orazio Taglialatela-Scafati |
| author_sort | Carmina Sirignano |
| collection | DOAJ |
| description | <i>Vitellaria paradoxa</i> C. F. Gaertn is widely used in African traditional medicine as an anti-inflammatory remedy to treat rheumatism, gastric problems, diarrhea, and dysentery. The phytochemical investigation of the ethyl acetate extract of <i>V. paradoxa</i> stem bark collected in Burkina Faso led to the isolation of eight known and two triterpenes undescribed to date (<b>7</b> and <b>10</b>), in the free alcohol form or as acetyl and cinnamyl ester derivatives. The stereostructures of the new compounds were elucidated using HR-ESIMS and 1D and 2D NMR data. The isolated compounds were evaluated <i>in vitro</i> for their inhibitory effect on nitrite levels on murine macrophages J774 stimulated with the lipopolysaccharide (LPS). Among all the compounds tested, lupeol cinnamate (<b>3</b>) and betulinic acid (<b>5</b>) showed a beneficial effect in reducing nitrite levels produced after LPS stimulation. |
| first_indexed | 2024-03-10T11:18:05Z |
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| id | doaj.art-64a1018525fb496a9693e6e0e4cbdde3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:18:05Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-64a1018525fb496a9693e6e0e4cbdde32023-11-21T20:15:54ZengMDPI AGPlants2223-77472021-05-01105100610.3390/plants10051006Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated MacrophagesCarmina Sirignano0Pascal Nadembega1Ferruccio Poli2Barbara Romano3Giuseppe Lucariello4Daniela Rigano5Orazio Taglialatela-Scafati6Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyThe UFR Life and Earth Sciences (UFR/SVT), University of Ouagadougou, Ouagadougou 848, Burkina FasoDepartment of Pharmacy and Biotechnology, University of Bologna, Via Irnerio, 42, 40126 Bologna, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy<i>Vitellaria paradoxa</i> C. F. Gaertn is widely used in African traditional medicine as an anti-inflammatory remedy to treat rheumatism, gastric problems, diarrhea, and dysentery. The phytochemical investigation of the ethyl acetate extract of <i>V. paradoxa</i> stem bark collected in Burkina Faso led to the isolation of eight known and two triterpenes undescribed to date (<b>7</b> and <b>10</b>), in the free alcohol form or as acetyl and cinnamyl ester derivatives. The stereostructures of the new compounds were elucidated using HR-ESIMS and 1D and 2D NMR data. The isolated compounds were evaluated <i>in vitro</i> for their inhibitory effect on nitrite levels on murine macrophages J774 stimulated with the lipopolysaccharide (LPS). Among all the compounds tested, lupeol cinnamate (<b>3</b>) and betulinic acid (<b>5</b>) showed a beneficial effect in reducing nitrite levels produced after LPS stimulation.https://www.mdpi.com/2223-7747/10/5/1006<i>Vitellaria paradoxa</i>triterpenescinnamyl estersnitrite level reduction |
| spellingShingle | Carmina Sirignano Pascal Nadembega Ferruccio Poli Barbara Romano Giuseppe Lucariello Daniela Rigano Orazio Taglialatela-Scafati Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages Plants <i>Vitellaria paradoxa</i> triterpenes cinnamyl esters nitrite level reduction |
| title | Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages |
| title_full | Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages |
| title_fullStr | Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages |
| title_full_unstemmed | Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages |
| title_short | Triterpenoids from <i>Vitellaria paradoxa</i> Stem Barks Reduce Nitrite Levels in LPS-Stimulated Macrophages |
| title_sort | triterpenoids from i vitellaria paradoxa i stem barks reduce nitrite levels in lps stimulated macrophages |
| topic | <i>Vitellaria paradoxa</i> triterpenes cinnamyl esters nitrite level reduction |
| url | https://www.mdpi.com/2223-7747/10/5/1006 |
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