Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats
Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goat...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/11/3/105 |
| _version_ | 1827304848766271488 |
|---|---|
| author | Carlos Velasco Javier Ortega Alberto Gómez-Buendía Anna Grau Marisol López Julio Álvarez Beatriz Romero Lucía de Juan Javier Bezos |
| author_facet | Carlos Velasco Javier Ortega Alberto Gómez-Buendía Anna Grau Marisol López Julio Álvarez Beatriz Romero Lucía de Juan Javier Bezos |
| author_sort | Carlos Velasco |
| collection | DOAJ |
| description | Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant increase (<i>p</i> < 0.001) in the quantitative response detected using an ELISA technique, conducted using serum and milk samples taken 15 and 30 days after performing a CITT (day 0 of the study); although, it did not translate into a significant increase in the number of reactors during any of the testing events (0, 3,15, 30 and 60 days post-CITT). Additionally, the number of ELISA-positive animals was higher for the serum versus the milk samples at both 15 and 30 days post-CITT. The increase in the quantitative ELISA result suggested a diagnostic strategy that maximizes ELISA sensitivity, mainly using serum samples, in PTB-infected herds; although, it may depend on individual differences and the interpretation criteria. |
| first_indexed | 2024-04-24T17:46:05Z |
| format | Article |
| id | doaj.art-64a4531274fe4ac3b72f7737147e6f07 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-04-24T17:46:05Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-64a4531274fe4ac3b72f7737147e6f072024-03-27T14:07:20ZengMDPI AGVeterinary Sciences2306-73812024-02-0111310510.3390/vetsci11030105Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from GoatsCarlos Velasco0Javier Ortega1Alberto Gómez-Buendía2Anna Grau3Marisol López4Julio Álvarez5Beatriz Romero6Lucía de Juan7Javier Bezos8VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainDirección General de Producción Agropecuaria e Infraestructuras Agrarias, Consejería de Agricultura y Ganadería de la Junta de Castilla y León, 47014 Valladolid, SpainServicio Territorial de Agricultura, Ganadería y Desarrollo Rural, 05001 Avila, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainParatuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant increase (<i>p</i> < 0.001) in the quantitative response detected using an ELISA technique, conducted using serum and milk samples taken 15 and 30 days after performing a CITT (day 0 of the study); although, it did not translate into a significant increase in the number of reactors during any of the testing events (0, 3,15, 30 and 60 days post-CITT). Additionally, the number of ELISA-positive animals was higher for the serum versus the milk samples at both 15 and 30 days post-CITT. The increase in the quantitative ELISA result suggested a diagnostic strategy that maximizes ELISA sensitivity, mainly using serum samples, in PTB-infected herds; although, it may depend on individual differences and the interpretation criteria.https://www.mdpi.com/2306-7381/11/3/105caprine paratuberculosiscomparative intradermal tuberculin testELISAbooster effecthumoral diagnosis |
| spellingShingle | Carlos Velasco Javier Ortega Alberto Gómez-Buendía Anna Grau Marisol López Julio Álvarez Beatriz Romero Lucía de Juan Javier Bezos Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats Veterinary Sciences caprine paratuberculosis comparative intradermal tuberculin test ELISA booster effect humoral diagnosis |
| title | Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats |
| title_full | Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats |
| title_fullStr | Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats |
| title_full_unstemmed | Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats |
| title_short | Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats |
| title_sort | evaluation of the effect of a recent comparative intradermal tuberculin test on the humoral diagnosis of paratuberculosis using serum and milk samples from goats |
| topic | caprine paratuberculosis comparative intradermal tuberculin test ELISA booster effect humoral diagnosis |
| url | https://www.mdpi.com/2306-7381/11/3/105 |
| work_keys_str_mv | AT carlosvelasco evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT javierortega evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT albertogomezbuendia evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT annagrau evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT marisollopez evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT julioalvarez evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT beatrizromero evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT luciadejuan evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats AT javierbezos evaluationoftheeffectofarecentcomparativeintradermaltuberculintestonthehumoraldiagnosisofparatuberculosisusingserumandmilksamplesfromgoats |