Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S]
Lipid and protein components of the stratum corneum (SC) are organized in complex supramolecular arrangements. Exploring spatial relations between various possible substructures is important for understanding the barrier function of this uppermost layer of epidermis. Here, we report the first study...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520349890 |
| _version_ | 1819293808470261760 |
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| author | Jean Doucet Anne Potter Carine Baltenneck Yegor A. Domanov |
| author_facet | Jean Doucet Anne Potter Carine Baltenneck Yegor A. Domanov |
| author_sort | Jean Doucet |
| collection | DOAJ |
| description | Lipid and protein components of the stratum corneum (SC) are organized in complex supramolecular arrangements. Exploring spatial relations between various possible substructures is important for understanding the barrier function of this uppermost layer of epidermis. Here, we report the first study where micro-focus X-ray scattering was used for assessing fine structural variations of the human skin barrier with micrometer resolution. We found that the scattering profiles were unchanged when scanning in the direction parallel to the SC surface. Furthermore, small-angle scattering profiles did not change as a function of depth in the SC, confirming that the lipid lamellar spacings remained the same throughout the SC. However, the wide-angle scattering data showed that the orthorhombic phase was more abundant in the middle layers of the SC, whereas the hexagonal phase dominated in the surface layers both at the external and the lowest part of the SC; i.e., the lipids were most tightly packed in the middle region of the SC. Taken together, our results demonstrate that microprobe X-ray diffraction provides abundant information about spatial variations of the SC lipid structure and thus may be a promising tool for assessing the effects of topical formulations on the barrier function of skin. |
| first_indexed | 2024-12-24T04:16:19Z |
| format | Article |
| id | doaj.art-64a4f0ebb4d4405ea5f7399bcfd2dc4e |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-24T04:16:19Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-64a4f0ebb4d4405ea5f7399bcfd2dc4e2022-12-21T17:15:56ZengElsevierJournal of Lipid Research0022-22752014-11-01551123802388Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S]Jean Doucet0Anne Potter1Carine Baltenneck2Yegor A. Domanov3Laboratoire de Physique des Solides, Université Paris-Sud, Orsay, France; Phi-Axis, Antony, FranceSkin Biophysics Laboratory, L'Oréal Research & Innovation, Aulnay-sous-Bois, FranceSkin Biophysics Laboratory, L'Oréal Research & Innovation, Aulnay-sous-Bois, FranceSkin Biophysics Laboratory, L'Oréal Research & Innovation, Aulnay-sous-Bois, France; To whom correspondence should be addressedLipid and protein components of the stratum corneum (SC) are organized in complex supramolecular arrangements. Exploring spatial relations between various possible substructures is important for understanding the barrier function of this uppermost layer of epidermis. Here, we report the first study where micro-focus X-ray scattering was used for assessing fine structural variations of the human skin barrier with micrometer resolution. We found that the scattering profiles were unchanged when scanning in the direction parallel to the SC surface. Furthermore, small-angle scattering profiles did not change as a function of depth in the SC, confirming that the lipid lamellar spacings remained the same throughout the SC. However, the wide-angle scattering data showed that the orthorhombic phase was more abundant in the middle layers of the SC, whereas the hexagonal phase dominated in the surface layers both at the external and the lowest part of the SC; i.e., the lipids were most tightly packed in the middle region of the SC. Taken together, our results demonstrate that microprobe X-ray diffraction provides abundant information about spatial variations of the SC lipid structure and thus may be a promising tool for assessing the effects of topical formulations on the barrier function of skin.http://www.sciencedirect.com/science/article/pii/S0022227520349890intercellular lipid lamellaebarrier functionmicrodiffractionheterogeneitydomainsceramide |
| spellingShingle | Jean Doucet Anne Potter Carine Baltenneck Yegor A. Domanov Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] Journal of Lipid Research intercellular lipid lamellae barrier function microdiffraction heterogeneity domains ceramide |
| title | Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] |
| title_full | Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] |
| title_fullStr | Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] |
| title_full_unstemmed | Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] |
| title_short | Micron-scale assessment of molecular lipid organization in human stratum corneum using microprobe X-ray diffraction[S] |
| title_sort | micron scale assessment of molecular lipid organization in human stratum corneum using microprobe x ray diffraction s |
| topic | intercellular lipid lamellae barrier function microdiffraction heterogeneity domains ceramide |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520349890 |
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