Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion
<i>Phaeodactylum tricornutum</i> is the marine diatom best known for high-value compounds that are useful in aquaculture and food area. In this study, fucoxanthin was first extracted from the diatom using supercritical fluid extraction (SFE) and then using the extracted diatom-like subst...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/2/127 |
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| author | Mari Carmen Ruiz-Domínguez Francisca Salinas Elena Medina Bárbara Rincón Marí Ángeles Martín Marí Carmen Gutiérrez Pedro Cerezal-Mezquita |
| author_facet | Mari Carmen Ruiz-Domínguez Francisca Salinas Elena Medina Bárbara Rincón Marí Ángeles Martín Marí Carmen Gutiérrez Pedro Cerezal-Mezquita |
| author_sort | Mari Carmen Ruiz-Domínguez |
| collection | DOAJ |
| description | <i>Phaeodactylum tricornutum</i> is the marine diatom best known for high-value compounds that are useful in aquaculture and food area. In this study, fucoxanthin was first extracted from the diatom using supercritical fluid extraction (SFE) and then using the extracted diatom-like substrate to produce bioenergy through anaerobic digestion (AD) processes. Factors such as temperature (30 °C and 50 °C), pressure (20, 30, and 40 MPa), and ethanol (co-solvent concentration from 10% to 50% <i>v/v</i>) were optimized for improving the yield, purity, and recovery of fucoxanthin extracted using SFE. The highest yield (24.41% <i>w/w</i>) was obtained at 30 MPa, 30 °C, and 30% ethanol but the highest fucoxanthin purity and recovery (85.03mg/g extract and 66.60% <i>w/w</i>, respectively) were obtained at 30 MPa, 30 °C, and 40%ethanol. Furthermore, ethanol as a factor had the most significant effect on the overall process of SFE. Subsequently, <i>P.tricornutum</i> biomass and SFE-extracted diatom were used as substrates for biogas production through AD. The effect of fucoxanthin was studied on the yield of AD, which resulted in 77.15 ± 3.85 LSTP CH<sub>4</sub>/kg volatile solids (VS) and 56.66 ± 1.90 LSTP CH<sub>4</sub>/kg VS for the whole diatom and the extracted <i>P.tricornutum</i>, respectively. Therefore, <i>P.tricornutuman</i> can be considered a potential source of fucoxanthin and methane and both productions will contribute to the sustainability of the algae-biorefinery processes. |
| first_indexed | 2024-03-09T21:33:21Z |
| format | Article |
| id | doaj.art-64a9063430a24ef3aed375683c8a690e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T21:33:21Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-64a9063430a24ef3aed375683c8a690e2023-11-23T20:50:17ZengMDPI AGMarine Drugs1660-33972022-02-0120212710.3390/md20020127Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic DigestionMari Carmen Ruiz-Domínguez0Francisca Salinas1Elena Medina2Bárbara Rincón3Marí Ángeles Martín4Marí Carmen Gutiérrez5Pedro Cerezal-Mezquita6Laboratorio de Microencapsulación de Compuestos Bioactivos (LAMICBA), Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta 1240000, ChileLaboratorio de Microencapsulación de Compuestos Bioactivos (LAMICBA), Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta 1240000, ChileLaboratorio de Microencapsulación de Compuestos Bioactivos (LAMICBA), Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta 1240000, ChileInstituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Campus Universidad Pablo de Olavide, Edificio 46. Ctra. de Utrera km. 1, 41013 Seville, SpainDepartamento de Química Inorgánica e Ingeniería Química, Universidad de Córdoba, Campus Universitario de Rabanales, 14071 Córdoba, SpainDepartamento de Química Inorgánica e Ingeniería Química, Universidad de Córdoba, Campus Universitario de Rabanales, 14071 Córdoba, SpainLaboratorio de Microencapsulación de Compuestos Bioactivos (LAMICBA), Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta 1240000, Chile<i>Phaeodactylum tricornutum</i> is the marine diatom best known for high-value compounds that are useful in aquaculture and food area. In this study, fucoxanthin was first extracted from the diatom using supercritical fluid extraction (SFE) and then using the extracted diatom-like substrate to produce bioenergy through anaerobic digestion (AD) processes. Factors such as temperature (30 °C and 50 °C), pressure (20, 30, and 40 MPa), and ethanol (co-solvent concentration from 10% to 50% <i>v/v</i>) were optimized for improving the yield, purity, and recovery of fucoxanthin extracted using SFE. The highest yield (24.41% <i>w/w</i>) was obtained at 30 MPa, 30 °C, and 30% ethanol but the highest fucoxanthin purity and recovery (85.03mg/g extract and 66.60% <i>w/w</i>, respectively) were obtained at 30 MPa, 30 °C, and 40%ethanol. Furthermore, ethanol as a factor had the most significant effect on the overall process of SFE. Subsequently, <i>P.tricornutum</i> biomass and SFE-extracted diatom were used as substrates for biogas production through AD. The effect of fucoxanthin was studied on the yield of AD, which resulted in 77.15 ± 3.85 LSTP CH<sub>4</sub>/kg volatile solids (VS) and 56.66 ± 1.90 LSTP CH<sub>4</sub>/kg VS for the whole diatom and the extracted <i>P.tricornutum</i>, respectively. Therefore, <i>P.tricornutuman</i> can be considered a potential source of fucoxanthin and methane and both productions will contribute to the sustainability of the algae-biorefinery processes.https://www.mdpi.com/1660-3397/20/2/127<i>Phaeodactylum tricornutum</i>supercritical fluid extractionco-solventfucoxanthinanaerobic digestionalgae biorefinery |
| spellingShingle | Mari Carmen Ruiz-Domínguez Francisca Salinas Elena Medina Bárbara Rincón Marí Ángeles Martín Marí Carmen Gutiérrez Pedro Cerezal-Mezquita Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion Marine Drugs <i>Phaeodactylum tricornutum</i> supercritical fluid extraction co-solvent fucoxanthin anaerobic digestion algae biorefinery |
| title | Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion |
| title_full | Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion |
| title_fullStr | Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion |
| title_full_unstemmed | Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion |
| title_short | Supercritical Fluid Extraction of Fucoxanthin from the Diatom <i>Phaeodactylum tricornutum</i> and Biogas Production through Anaerobic Digestion |
| title_sort | supercritical fluid extraction of fucoxanthin from the diatom i phaeodactylum tricornutum i and biogas production through anaerobic digestion |
| topic | <i>Phaeodactylum tricornutum</i> supercritical fluid extraction co-solvent fucoxanthin anaerobic digestion algae biorefinery |
| url | https://www.mdpi.com/1660-3397/20/2/127 |
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