Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer
Objective To explore the potential prognostic role and value of autophagy-related lncRNA risk prediction signature in patients with bladder cancer. Methods The transcriptome data and corresponding clinical data of patients with bladder cancer were obtained from the Cancer Genome Atlas (TCGA) databas...
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| Format: | Article |
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Editorial Office of Journal of Third Military Medical University
2021-12-01
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| Series: | Di-san junyi daxue xuebao |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202106120.htm |
| _version_ | 1818742411538464768 |
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| author | TONG Hang LI Tinghao CAO Honghao LIU Xudong ZHU Junlong QIN Zijia YIN Siwen HE Weiyang |
| author_facet | TONG Hang LI Tinghao CAO Honghao LIU Xudong ZHU Junlong QIN Zijia YIN Siwen HE Weiyang |
| author_sort | TONG Hang |
| collection | DOAJ |
| description | Objective To explore the potential prognostic role and value of autophagy-related lncRNA risk prediction signature in patients with bladder cancer. Methods The transcriptome data and corresponding clinical data of patients with bladder cancer were obtained from the Cancer Genome Atlas (TCGA) database. Four hundreds and two bladder cancer samples were randomly divided into training set and verification set according to the ratio of 3 ∶1 by R software. Autophagy-related lncRNA, was screened by Pearson correlation analysis, and autophagy-related lncRNA prognostic signature was constructed by univariate and multivariate Cox regression analyses. The accuracy of the signature was evaluated by drawing ROC curve. The sample risk score was further calculated, and the relationship of the sample risk score with clinical characteristics and survival prognosis was analyzed. In addition, the ability of the signature to predict the prognosis of patients with bladder cancer was evaluated by constructing a nomogram. Finally, the possible enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in different risk groups were analyzed by gene set enrichment analysis (GSEA). Finally, the expression of high-risk lncRNA was verified in the signature by RT-qPCR. Results An 8-autophagy-related lncRNA signature was successfully constructed. In the training set, validation set and whole set, the AUC values of the ROC curve of the prognostic signature were all greater than 0.75. The risk score of the signature was related to age, American Joint Committee on Cancer (AJCC) stage and T stage of the patients (P < 0.05), but not to their gender. The nomogram established by integrating clinical variables and risk scores confirmed that the signature had a good ability to predict prognosis. GSEA showed that some tumor and autophagy-related pathways were enriched in the high-risk group, while immune-related pathways were enriched in the low-risk group. lncRNA AC005229.4 was highly expressed in bladder cancer cell lines. Conclusion An 8-autophagy-related lncRNA signature is effective in the prognostic prediction of patients with bladder can |
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| id | doaj.art-64a99b62313944aabfa11edf33b99606 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-18T02:12:06Z |
| publishDate | 2021-12-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
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| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-64a99b62313944aabfa11edf33b996062022-12-21T21:24:29ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042021-12-0143232532254110.16016/j.1000-5404.202106120Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancerTONG Hang0LI Tinghao1CAO Honghao2 LIU Xudong3ZHU Junlong4QIN Zijia5YIN Siwen6 HE Weiyang7Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Department of Urology, Rongchang Traditional Chinese Medicine Hospital, Chongqing, 402460Department of Urology, Bishan Traditional Chinese Medicine Hospital, Chongqing, 402760, ChinaDepartment of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016Objective To explore the potential prognostic role and value of autophagy-related lncRNA risk prediction signature in patients with bladder cancer. Methods The transcriptome data and corresponding clinical data of patients with bladder cancer were obtained from the Cancer Genome Atlas (TCGA) database. Four hundreds and two bladder cancer samples were randomly divided into training set and verification set according to the ratio of 3 ∶1 by R software. Autophagy-related lncRNA, was screened by Pearson correlation analysis, and autophagy-related lncRNA prognostic signature was constructed by univariate and multivariate Cox regression analyses. The accuracy of the signature was evaluated by drawing ROC curve. The sample risk score was further calculated, and the relationship of the sample risk score with clinical characteristics and survival prognosis was analyzed. In addition, the ability of the signature to predict the prognosis of patients with bladder cancer was evaluated by constructing a nomogram. Finally, the possible enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in different risk groups were analyzed by gene set enrichment analysis (GSEA). Finally, the expression of high-risk lncRNA was verified in the signature by RT-qPCR. Results An 8-autophagy-related lncRNA signature was successfully constructed. In the training set, validation set and whole set, the AUC values of the ROC curve of the prognostic signature were all greater than 0.75. The risk score of the signature was related to age, American Joint Committee on Cancer (AJCC) stage and T stage of the patients (P < 0.05), but not to their gender. The nomogram established by integrating clinical variables and risk scores confirmed that the signature had a good ability to predict prognosis. GSEA showed that some tumor and autophagy-related pathways were enriched in the high-risk group, while immune-related pathways were enriched in the low-risk group. lncRNA AC005229.4 was highly expressed in bladder cancer cell lines. Conclusion An 8-autophagy-related lncRNA signature is effective in the prognostic prediction of patients with bladder canhttp://aammt.tmmu.edu.cn/Upload/rhtml/202106120.htmbladder cancerbioinformaticsautophagylncrnaprognosis signature |
| spellingShingle | TONG Hang LI Tinghao CAO Honghao LIU Xudong ZHU Junlong QIN Zijia YIN Siwen HE Weiyang Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer Di-san junyi daxue xuebao bladder cancer bioinformatics autophagy lncrna prognosis signature |
| title | Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer |
| title_full | Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer |
| title_fullStr | Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer |
| title_full_unstemmed | Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer |
| title_short | Role of 8-autophagy-related long non-coding RNAs signature in prognosis for bladder cancer |
| title_sort | role of 8 autophagy related long non coding rnas signature in prognosis for bladder cancer |
| topic | bladder cancer bioinformatics autophagy lncrna prognosis signature |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202106120.htm |
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