<i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand
Dissemination of the mobile colistin resistance gene <i>mcr</i> in <i>Enterobacterales</i> among humans, animals, and the environment is a public health issue. We characterized <i>mcr</i> genes in the <i>Klebsiella</i> <i>pneumoniae</i> com...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/12/2436 |
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| author | Nattamol Phetburom Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Yukihiro Akeda Shigeyuki Hamada Suphachai Nuanualsuwan Anusak Kerdsin |
| author_facet | Nattamol Phetburom Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Yukihiro Akeda Shigeyuki Hamada Suphachai Nuanualsuwan Anusak Kerdsin |
| author_sort | Nattamol Phetburom |
| collection | DOAJ |
| description | Dissemination of the mobile colistin resistance gene <i>mcr</i> in <i>Enterobacterales</i> among humans, animals, and the environment is a public health issue. We characterized <i>mcr</i> genes in the <i>Klebsiella</i> <i>pneumoniae</i> complex (KpnC) isolated from slaughtered pigs in Thailand. The 280 KpnCs consisted of <i>K. pneumoniae</i> (85%), <i>Klebsiella quasipneumoniae</i> (8.21%), and <i>Klebsiella variicola</i> (6.79%). <i>mcr</i> genes were detected in 6.79% (19/280) of KpnC isolates, consisting of <i>mcr-8</i> (<i>n</i> = 9; 3.21%), <i>mcr-7</i> (<i>n</i> = 7; 2.50%), <i>mcr-7</i> + <i>mcr-8</i> (<i>n</i> = 2; 0.71%), and <i>mcr-1</i> + <i>mcr-7</i> (<i>n</i> = 1; 0.36%). <i>K. pneumoniae</i> predominantly carried the <i>mcr-7</i> and <i>mcr-8</i> genes, while <i>K. variicola</i> and <i>K. quasipneumoniae</i> harbored <i>mcr-7</i> and <i>mcr-8,</i> respectively. Six of the nineteen <i>mcr</i>-harboring KpnC isolates exhibited colistin resistance, and five had <i>mcr-1</i> or <i>mcr-8</i> transferable to an <i>Escherichia coli</i> recipient. Antimicrobial susceptibility analysis revealed that all <i>mcr</i>-carrying KpnC isolates were susceptible to carbapenems, cefotaxime, cefepime, amoxicillin/clavulanic acid, piperacillin/tazobactam, amikacin, and fosfomycin, and had high resistance to azithromycin. Multilocus sequence analysis demonstrated that the <i>mcr</i>-harboring KpnC isolates were genetically diverse. A ‘One-Health’ approach is useful to combat antimicrobial-resistant bacteria through coordinating the human, animal, and environmental sectors. Hence, continuous monitoring and surveillance of <i>mcr-</i>carrying KpnCs throughout the pork supply chain is crucial for ensuring public health. |
| first_indexed | 2024-03-10T03:32:18Z |
| format | Article |
| id | doaj.art-64a9a305e7c144c2a049eea8b190b531 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:32:18Z |
| publishDate | 2021-11-01 |
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| series | Microorganisms |
| spelling | doaj.art-64a9a305e7c144c2a049eea8b190b5312023-11-23T09:37:58ZengMDPI AGMicroorganisms2076-26072021-11-01912243610.3390/microorganisms9122436<i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in ThailandNattamol Phetburom0Parichart Boueroy1Peechanika Chopjitt2Rujirat Hatrongjit3Yukihiro Akeda4Shigeyuki Hamada5Suphachai Nuanualsuwan6Anusak Kerdsin7Faculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandFaculty of Science and Engineering, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandJapan-Thailand Research Collaboration Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, JapanJapan-Thailand Research Collaboration Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandFaculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandDissemination of the mobile colistin resistance gene <i>mcr</i> in <i>Enterobacterales</i> among humans, animals, and the environment is a public health issue. We characterized <i>mcr</i> genes in the <i>Klebsiella</i> <i>pneumoniae</i> complex (KpnC) isolated from slaughtered pigs in Thailand. The 280 KpnCs consisted of <i>K. pneumoniae</i> (85%), <i>Klebsiella quasipneumoniae</i> (8.21%), and <i>Klebsiella variicola</i> (6.79%). <i>mcr</i> genes were detected in 6.79% (19/280) of KpnC isolates, consisting of <i>mcr-8</i> (<i>n</i> = 9; 3.21%), <i>mcr-7</i> (<i>n</i> = 7; 2.50%), <i>mcr-7</i> + <i>mcr-8</i> (<i>n</i> = 2; 0.71%), and <i>mcr-1</i> + <i>mcr-7</i> (<i>n</i> = 1; 0.36%). <i>K. pneumoniae</i> predominantly carried the <i>mcr-7</i> and <i>mcr-8</i> genes, while <i>K. variicola</i> and <i>K. quasipneumoniae</i> harbored <i>mcr-7</i> and <i>mcr-8,</i> respectively. Six of the nineteen <i>mcr</i>-harboring KpnC isolates exhibited colistin resistance, and five had <i>mcr-1</i> or <i>mcr-8</i> transferable to an <i>Escherichia coli</i> recipient. Antimicrobial susceptibility analysis revealed that all <i>mcr</i>-carrying KpnC isolates were susceptible to carbapenems, cefotaxime, cefepime, amoxicillin/clavulanic acid, piperacillin/tazobactam, amikacin, and fosfomycin, and had high resistance to azithromycin. Multilocus sequence analysis demonstrated that the <i>mcr</i>-harboring KpnC isolates were genetically diverse. A ‘One-Health’ approach is useful to combat antimicrobial-resistant bacteria through coordinating the human, animal, and environmental sectors. Hence, continuous monitoring and surveillance of <i>mcr-</i>carrying KpnCs throughout the pork supply chain is crucial for ensuring public health.https://www.mdpi.com/2076-2607/9/12/2436<i>Klebsiella pneumoniae</i><i>mcr</i>colistinpigsThailand |
| spellingShingle | Nattamol Phetburom Parichart Boueroy Peechanika Chopjitt Rujirat Hatrongjit Yukihiro Akeda Shigeyuki Hamada Suphachai Nuanualsuwan Anusak Kerdsin <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand Microorganisms <i>Klebsiella pneumoniae</i> <i>mcr</i> colistin pigs Thailand |
| title | <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand |
| title_full | <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand |
| title_fullStr | <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand |
| title_full_unstemmed | <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand |
| title_short | <i>Klebsiella pneumoniae</i> Complex Harboring <i>mcr-1</i>, <i>mcr-7,</i> and <i>mcr-8</i> Isolates from Slaughtered Pigs in Thailand |
| title_sort | i klebsiella pneumoniae i complex harboring i mcr 1 i i mcr 7 i and i mcr 8 i isolates from slaughtered pigs in thailand |
| topic | <i>Klebsiella pneumoniae</i> <i>mcr</i> colistin pigs Thailand |
| url | https://www.mdpi.com/2076-2607/9/12/2436 |
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