A new method for quantifying mitochondrial axonal transport
ABSTRACT Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microf...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2016-05-01
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| Series: | Protein & Cell |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s13238-016-0268-3 |
| _version_ | 1797724977301028864 |
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| author | Mengmeng Chen Yang Li Mengxue Yang Xiaoping Chen Yemeng Chen Fan Yang Sheng Lu Shengyu Yao Timothy Zhou Jianghong Liu Li Zhu Sidan Du Jane Y. Wu |
| author_facet | Mengmeng Chen Yang Li Mengxue Yang Xiaoping Chen Yemeng Chen Fan Yang Sheng Lu Shengyu Yao Timothy Zhou Jianghong Liu Li Zhu Sidan Du Jane Y. Wu |
| author_sort | Mengmeng Chen |
| collection | DOAJ |
| description | ABSTRACT Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named “MitoQuant”. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses. |
| first_indexed | 2024-03-12T10:23:42Z |
| format | Article |
| id | doaj.art-64aa2bbe10a84a03b2d9fc6dd9517410 |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T10:23:42Z |
| publishDate | 2016-05-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-64aa2bbe10a84a03b2d9fc6dd95174102023-09-02T09:53:01ZengOxford University PressProtein & Cell1674-800X1674-80182016-05-0171180481910.1007/s13238-016-0268-3A new method for quantifying mitochondrial axonal transportMengmeng Chen0Yang Li1Mengxue Yang2Xiaoping Chen3Yemeng Chen4Fan Yang5Sheng Lu6Shengyu Yao7Timothy Zhou8Jianghong Liu9Li Zhu10Sidan Du11Jane Y. Wu12University of Chinese Academy of SciencesSchool of Electronic Science & Engineering, Nanjing UniversityUniversity of Chinese Academy of SciencesDepartment of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of MedicineSchool of Electronic Science & Engineering, Nanjing UniversitySchool of Electronic Science & Engineering, Nanjing UniversitySchool of Electronic Science & Engineering, Nanjing UniversityDepartment of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of MedicineDepartment of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of MedicineState Key Laboratory for Brain & Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesState Key Laboratory for Brain & Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesSchool of Electronic Science & Engineering, Nanjing UniversityState Key Laboratory for Brain & Cognitive Science, Institute of Biophysics, Chinese Academy of SciencesABSTRACT Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named “MitoQuant”. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses.http://link.springer.com/article/10.1007/s13238-016-0268-3mitochondrial transportimage processing and analysisFUS proteinopathy and mitochondrial transport defect |
| spellingShingle | Mengmeng Chen Yang Li Mengxue Yang Xiaoping Chen Yemeng Chen Fan Yang Sheng Lu Shengyu Yao Timothy Zhou Jianghong Liu Li Zhu Sidan Du Jane Y. Wu A new method for quantifying mitochondrial axonal transport Protein & Cell mitochondrial transport image processing and analysis FUS proteinopathy and mitochondrial transport defect |
| title | A new method for quantifying mitochondrial axonal transport |
| title_full | A new method for quantifying mitochondrial axonal transport |
| title_fullStr | A new method for quantifying mitochondrial axonal transport |
| title_full_unstemmed | A new method for quantifying mitochondrial axonal transport |
| title_short | A new method for quantifying mitochondrial axonal transport |
| title_sort | new method for quantifying mitochondrial axonal transport |
| topic | mitochondrial transport image processing and analysis FUS proteinopathy and mitochondrial transport defect |
| url | http://link.springer.com/article/10.1007/s13238-016-0268-3 |
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